H5C6

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$40.00
SKU: H5C6
View product citations for antibody H5C6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD63 (human) LAMP-3
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: August, J.T. / Hildreth, J.E.K.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University School of Medicine
Positive Tested Species Reactivity: Human, Porcine
Depositors Notes:
Antigen Molecular Weight: Predicted: 26 kDa; Apparent: heterodisperse glycoprotein of usually 40 to 55 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CD63
Immunogen: Human adherent spleen cells
Alternate Gene Names: MLA1, TSPAN30, ME491, LAMP-3, OMA81H
Alternate Antibody Name: anti-Pltg~4
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X653-Ag8
Epitope Location or Sequence: Recognizes an extracellular epitope: extracellular domain 1 or 2, corresponding to aa 33-51 and 103-203, respectively.
Uniprot ID: P08962 
Immunogen Sequence: Full length protein
Entrez Gene ID: 967 
Additional Characterization:  
Antibody Registry ID: AB_528158 
Additional Information: RRID:AB_528158
Recommended Applications: ELISA, FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
H5C6 was deposited to the DSHB by August, J.T. / Hildreth, J.E.K. (DSHB Hybridoma Product H5C6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References

    Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions.
    Rubinstein E
    The Biochemical journal 340 ( Pt 1). (1999 May 15): 103-11.

    High levels of exosomes expressing CD63 and caveolin-1 in plasma of melanoma patients.
    Fais S
    PloS one 4.4 (2009): e5219.

    The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.
    Caplan MJ
    Proceedings of the National Academy of Sciences of the United States of America 100.26 (2003 Dec 23): 15560-5.

    A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication.
    Linē A
    Biochemical and biophysical research communications 495.2 (2018 Jan 8): 1930-1935.

    Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
    Rivoltini L
    Gastroenterology 128.7 (2005 Jun): 1796-804.

    Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
    Falcon-Perez JM
    Journal of proteomics 75.12 (2012 Jun 27): 3574-84.

    Profiling of microRNAs in exosomes released from PC-3 prostate cancer cells.
    Llorente A
    Biochimica et biophysica acta 1819.11-12 (2012 Nov-Dec): 1154-63.

    Palmitoylation-dependent association with CD63 targets the Ca2+ sensor synaptotagmin VII to lysosomes.
    Andrews NW
    The Journal of cell biology 191.3 (2010 Nov 1): 599-613.

    The tetraspan protein CD82 is a resident of MHC class II compartments where it associates with HLA-DR, -DM, and -DO molecules.
    Cresswell P
    Journal of immunology (Baltimore, Md. : 1950) 161.7 (1998 Oct 1): 3282-91.

    Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture.
    Bowes Rickman C
    Advances in experimental medicine and biology 1185. (2019): 21-25.

    Surface-Enhanced Raman Spectroscopy as a Tool for Distinguishing Extracellular Vesicles under Autophagic Conditions: A Marker for Disease Diagnostics.
    Narayana C
    The journal of physical chemistry. B 124.48 (2020 Dec 3): 10952-10960.

    IP References
    ELISA References
    FB References
    FACS References
    All References

    The dynamics of autophagy visualized in live cells: from autophagosome formation to fusion with endo/lysosomes.
    Tolkovsky AM
    Autophagy 1.1 (2005 Apr): 23-36.

    Crimean-Congo hemorrhagic fever virus entry into host cells occurs through the multivesicular body and requires ESCRT regulators.
    Davey RA
    PLoS pathogens 10.9 (2014 Sep): e1004390.

    Early phagosomes in dendritic cells form a cellular compartment sufficient for cross presentation of exogenous antigens.
    Cresswell P
    Proceedings of the National Academy of Sciences of the United States of America 100.22 (2003 Oct 28): 12889-94.

    Enzymatic reduction of disulfide bonds in lysosomes: characterization of a gamma-interferon-inducible lysosomal thiol reductase (GILT).
    Cresswell P
    Proceedings of the National Academy of Sciences of the United States of America 97.2 (2000 Jan 18): 745-50.

    The tetraspan protein CD82 is a resident of MHC class II compartments where it associates with HLA-DR, -DM, and -DO molecules.
    Cresswell P
    Journal of immunology (Baltimore, Md. : 1950) 161.7 (1998 Oct 1): 3282-91.

    Biogenesis of lysosome-related organelles complex-1 (BORC) regulates late endosomal/lysosomal size through PIKfyve-dependent phosphatidylinositol-3,5-bisphosphate.
    Huber LA
    Traffic (Copenhagen, Denmark) 20.9 (2019 Sep): 674-696.

    Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions.
    Rubinstein E
    The Biochemical journal 340 ( Pt 1). (1999 May 15): 103-11.

    High levels of exosomes expressing CD63 and caveolin-1 in plasma of melanoma patients.
    Fais S
    PloS one 4.4 (2009): e5219.

    The tetraspanin CD63 enhances the internalization of the H,K-ATPase beta-subunit.
    Caplan MJ
    Proceedings of the National Academy of Sciences of the United States of America 100.26 (2003 Dec 23): 15560-5.

    A novel 3D heterotypic spheroid model for studying extracellular vesicle-mediated tumour and immune cell communication.
    Linē A
    Biochemical and biophysical research communications 495.2 (2018 Jan 8): 1930-1935.

    Human colorectal cancer cells induce T-cell death through release of proapoptotic microvesicles: role in immune escape.
    Rivoltini L
    Gastroenterology 128.7 (2005 Jun): 1796-804.

    Proteomic analysis of microvesicles from plasma of healthy donors reveals high individual variability.
    Falcon-Perez JM
    Journal of proteomics 75.12 (2012 Jun 27): 3574-84.

    Profiling of microRNAs in exosomes released from PC-3 prostate cancer cells.
    Llorente A
    Biochimica et biophysica acta 1819.11-12 (2012 Nov-Dec): 1154-63.

    Palmitoylation-dependent association with CD63 targets the Ca2+ sensor synaptotagmin VII to lysosomes.
    Andrews NW
    The Journal of cell biology 191.3 (2010 Nov 1): 599-613.

    Isolation of Retinal Exosome Biomarkers from Blood by Targeted Immunocapture.
    Bowes Rickman C
    Advances in experimental medicine and biology 1185. (2019): 21-25.

    Surface-Enhanced Raman Spectroscopy as a Tool for Distinguishing Extracellular Vesicles under Autophagic Conditions: A Marker for Disease Diagnostics.
    Narayana C
    The journal of physical chemistry. B 124.48 (2020 Dec 3): 10952-10960.

    Characterization of a novel self-associating Mr 40,000 platelet glycoprotein.
    Azorsa DO
    Blood 77.1 (1991 Jan 1): 121-32.

    CD63/Pltgp40: a platelet activation antigen identical to the stage-specific, melanoma-associated antigen ME491.
    Hildreth JE
    Blood 78.2 (1991 Jul 15): 280-4.

    Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.
    Partridge LJ
    Immunology 127.2 (2009 Jun): 237-48.

    Tetraspanin CD9 is associated with very late-acting integrins in human vascular smooth muscle cells and modulates collagen matrix reorganization.
    Beretz A
    Arteriosclerosis, thrombosis, and vascular biology 18.11 (1998 Nov): 1691-7.

    RNA-containing exosomes in human nasal secretions.
    Lötvall J
    American journal of rhinology & allergy 25.2 (2011 Mar-Apr): 89-93.

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