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View product citations for antibody BDNF-#1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Mature brain-derived neurotrophic factor
Hybridoma Cells Available: Yes
Antigen Species: Fish/Human
Depositor: Barde, Y.-A.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Biozentrum University of Basel
Positive Tested Species Reactivity: Human, Mammal, Mouse, Rat
Depositors Notes: This antibody doesn't work for Immunostaining nor function blocking. Works for immunoprecipitation but BDNF-#9 is preferred.
Antigen Molecular Weight: Apparent: 14 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: BDNF
Immunogen: Fish and Human BDNF
Alternate Gene Names:
Alternate Antibody Name: mAb#1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63.Ag 8.635
Epitope Location or Sequence:
Uniprot ID: P23560 
Immunogen Sequence: Full length protein
Entrez Gene ID: 627 
Additional Characterization:  
Antibody Registry ID: AB_2617198 
Additional Information: For ELISA, BDNF-#1 is often used in combination with BDNF-#9 (a different clone from the same fusion; available in the DSHB collection) that recognizes a different epitope on BDNF.
Recommended Applications: ELISA, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BDNF-#1 was deposited to the DSHB by Barde, Y.-A. (DSHB Hybridoma Product BDNF-#1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • WB References
  • ELISA References
  • All References
  • Initial Publication
    WB References
    ELISA References
    All References

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