BDNF-#9
Catalog Fields
Clone ID/Product Name: BDNF-#9
Available to For-Profits: Yes
Alternate Antibody Name: mAb#9
Gene Symbol: BDNF
Ab Isotype: MIgG2b
Gene Name:
Antibody Registry ID: AB_2617199
Uniprot ID: P23560
Entrez Gene ID: 627
Clonality: Monoclonal
Immunogen: Fish and Human BDNF
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: X63.Ag 8.635
Epitope Mapped: No
Antigen Name: Mature brain-derived neurotrophic factor
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/2/2012
Antigen Molecular Weight: Apparent: 14 kDa
Depositor: Barde, Y.-A.
Antigen Sequence:
Depositor Institution: University of Basel, Biozentrum
Antigen Species: Fish/Human
Depositor Notes: BDNF-#9 blocks the biological activity of Xyphophorus and mammalian BDNF. For immunostaining: perfusion fixation with 4% paraformaldehyde, 0.1% glutaraldehyde, 0.3% picric acid.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Fish, Human, Mammal, Mouse, Rat
Additional Information: BDNF-#9 does not recognize BDNF on western blots but mAb BDNF-#1 (a different clone from the same fusion; available in the DSHB collection) reacts specifically with BDNF by western blot. BDNF-#9 doesn't appear to recognize chick BDNF. This antibody also recognizes pro-BDNF. For ELISA, BDNF-#9 is often used in combination with BDNF-#1 that recognizes a different epitope on BDNF.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation