BDNF-#9

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$40.00
SKU: BDNF-#9
View product citations for antibody BDNF-#9 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Mature brain-derived neurotrophic factor
Hybridoma Cells Available: Yes
Antigen Species: Fish/Human
Depositor: Barde, Y.-A.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Biozentrum University of Basel
Positive Tested Species Reactivity: Fish, Human, Mammal, Mouse, Rat
Depositors Notes: BDNF-#9 blocks the biological activity of Xyphophorus and mammalian BDNF. For immunostaining: perfusion fixation with 4% paraformaldehyde, 0.1% glutaraldehyde, 0.3% picric acid.
Antigen Molecular Weight: Apparent: 14 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: BDNF
Immunogen: Fish and Human BDNF
Alternate Gene Names:
Alternate Antibody Name: mAb#9
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: X63.Ag 8.635
Epitope Location or Sequence:
Uniprot ID: P23560 
Immunogen Sequence: Full length protein
Entrez Gene ID: 627 
Additional Characterization:  
Antibody Registry ID: AB_2617199 
Additional Information: BDNF-#9 does not recognize BDNF on western blots but mAb BDNF-#1 (a different clone from the same fusion; available in the DSHB collection) reacts specifically with BDNF by western blot. BDNF-#9 doesn't appear to recognize chick BDNF. This antibody also recognizes pro-BDNF. For ELISA, BDNF-#9 is often used in combination with BDNF-#1 that recognizes a different epitope on BDNF.
Recommended Applications: ELISA, Function Blocking, Immunofluorescence, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
BDNF-#9 was deposited to the DSHB by Barde, Y.-A. (DSHB Hybridoma Product BDNF-#9)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • IP References
  • ELISA References
  • FB References
  • All References
  • Initial Publication
    IF References
    IP References

    Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.
    Barde YA
    Nature neuroscience 11.2 (2008 Feb): 131-3.

    Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.
    Barde YA
    Nature neuroscience 11.2 (2008 Feb): 131-3.

    BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
    Barde YA
    The Journal of cell biology 196.6 (2012 Mar 19): 775-88.

    Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome.
    Barde YA
    Proceedings of the National Academy of Sciences of the United States of America 109.35 (2012 Aug 28): 14230-5.

    Electroconvulsive seizure, but not imipramine, rapidly up-regulates pro-BDNF and t-PA, leading to mature BDNF production, in the rat hippocampus.
    Yamawaki S
    The international journal of neuropsychopharmacology 16.2 (2013 Mar): 339-50.

    ELISA References
    FB References

    Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice.
    Barde YA
    Journal of neurochemistry 72.5 (1999 May): 1930-8.

    Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome.
    Barde YA
    Proceedings of the National Academy of Sciences of the United States of America 109.35 (2012 Aug 28): 14230-5.

    BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin.
    Lo DC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.3 (2014 Jan 15): 963-8.

    NGF-Dependent and BDNF-Dependent DRG Sensory Neurons Deploy Distinct Degenerative Signaling Mechanisms.
    Barker PA
    eNeuro 8.1 (2021 Jan-Feb): .

    All References

    BDNF and its pro-peptide are stored in presynaptic dense core vesicles in brain neurons.
    Barde YA
    The Journal of cell biology 196.6 (2012 Mar 19): 775-88.

    High abundance of BDNF within glutamatergic presynapses of cultured hippocampal neurons.
    Blum R
    Frontiers in cellular neuroscience 8. (2014): 107.

    Glutamatergic axon-derived BDNF controls GABAergic synaptic differentiation in the cerebellum.
    Reichardt LF
    Scientific reports 6. (2016 Feb 1): 20201.

    Biosynthesis and processing of endogenous BDNF: CNS neurons store and secrete BDNF, not pro-BDNF.
    Barde YA
    Nature neuroscience 11.2 (2008 Feb): 131-3.

    Fingolimod, a sphingosine-1 phosphate receptor modulator, increases BDNF levels and improves symptoms of a mouse model of Rett syndrome.
    Barde YA
    Proceedings of the National Academy of Sciences of the United States of America 109.35 (2012 Aug 28): 14230-5.

    Electroconvulsive seizure, but not imipramine, rapidly up-regulates pro-BDNF and t-PA, leading to mature BDNF production, in the rat hippocampus.
    Yamawaki S
    The international journal of neuropsychopharmacology 16.2 (2013 Mar): 339-50.

    Brain-derived neurotrophic factor levels in the nervous system of wild-type and neurotrophin gene mutant mice.
    Barde YA
    Journal of neurochemistry 72.5 (1999 May): 1930-8.

    The impact of Bdnf gene deficiency to the memory impairment and brain pathology of APPswe/PS1dE9 mouse model of Alzheimer's disease.
    Castrén E
    PloS one 8.7 (2013): e68722.

    Hippocampal enlargement in Bassoon-mutant mice is associated with enhanced neurogenesis, reduced apoptosis, and abnormal BDNF levels.
    Gundelfinger ED
    Cell and tissue research 346.1 (2011 Oct): 11-26.

    Sonic Hedgehog Promotes Neurite Outgrowth of Primary Cortical Neurons Through Up-Regulating BDNF Expression.
    Xie Y
    Neurochemical research 41.4 (2016 Apr): 687-95.

    BDNF mediates neuroprotection against oxygen-glucose deprivation by the cardiac glycoside oleandrin.
    Lo DC
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.3 (2014 Jan 15): 963-8.

    NGF-Dependent and BDNF-Dependent DRG Sensory Neurons Deploy Distinct Degenerative Signaling Mechanisms.
    Barker PA
    eNeuro 8.1 (2021 Jan-Feb): .

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