Catalog Fields
Antigen: Myosin heavy chain, Type IIB cytoplasmic non-muscle
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Conrad, G.W. / Conrad, A.H.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Kansas State University
Positive Tested Species Reactivity: Chick
Depositors Notes: dot blot yes, but does not recognize SDS-denatured myosin
Predicted Species Reactivity:
Gene:
Immunogen: cytoplasmic nonmuscle myosin II
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Ag8.653
Epitope Location or Sequence:
Antibody Registry ID: AB_2147143
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CMII 25 was deposited to the DSHB by Conrad, G.W. / Conrad, A.H. (DSHB Hybridoma Product CMII 25)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis.
Conrad GW
Cell motility and the cytoskeleton 19.3 (1991): 189-206.