CMII 23

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$40.00
SKU: CMII 23
View product citations for antibody CMII 23 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: myosin IIB, cytoplasmic non-muscle
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Conrad, G.W. / Conrad, A.H.
Isotype: MIgG1, kappa light chain
Depositors Institution: Kansas State University
Host Species: mouse
Depositors Notes: The polypeptide eptiope recognized by H4C4 is present in both CD44s and CD44v proteins.
Positive Tested Species Reactivity: Bovine, Chicken, Human, Mouse, Rat
Gene: MYH10
Antigen Molecular Weight: 232.6 kDa
Clonality: Monoclonal
Immunogen: Myosin purified from chick brain
Epitope Mapped: No
Myeloma Strain: Ag 8.653
Immunogen Sequence: Full length protein
Uniprot ID: Q789A4 
Additional Information: RRID: AB_528359
Entrez Gene ID: 396465 
Antibody Registry ID: AB_528359 
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CMII 23 was deposited to the DSHB by Conrad, G.W. / Conrad, A.H. (DSHB Hybridoma Product CMII 23)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

25 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • All References
  • Initial Publication
    IF References

    Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis.
    Conrad GW
    Cell motility and the cytoskeleton 19.3 (1991): 189-206.

    Differential localization of cytoplasmic myosin II isoforms A and B in avian interphase and dividing embryonic and immortalized cardiomyocytes and other cell types in vitro.
    Conrad GW
    Cell motility and the cytoskeleton 31.2 (1995): 93-112.

    Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network.
    Hildebrand JD
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5191-203.

    Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins.
    Hildebrand JD
    The Journal of biological chemistry 281.29 (2006 Jul 21): 20542-54.

    Shroom4 (Kiaa1202) is an actin-associated protein implicated in cytoskeletal organization.
    Hildebrand JD
    Cell motility and the cytoskeleton 64.1 (2007 Jan): 49-63.

    Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
    Sheetz MP
    Biophysical journal 91.10 (2006 Nov 15): 3907-20.

    Role of nonmuscle myosin IIB and N-RAP in cell spreading and myofibril assembly in primary mouse cardiomyocytes.
    Horowits R
    Cell motility and the cytoskeleton 65.9 (2008 Sep): 747-61.

    Spectral properties and mechanisms that underlie autofluorescent accumulations in Batten disease.
    Pearce DA
    Biochemical and biophysical research communications 382.2 (2009 May 1): 247-51.

    Cholangiocyte myosin IIB is required for localized aggregation of sodium glucose cotransporter 1 to sites of Cryptosporidium parvum cellular invasion and facilitates parasite internalization.
    LaRusso NF
    Infection and immunity 78.7 (2010 Jul): 2927-36.

    A novel interaction of CLN3 with nonmuscle myosin-IIB and defects in cell motility of Cln3(-/-) cells.
    Pearce DA
    Experimental cell research 317.1 (2011 Jan 1): 51-69.

    Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions.
    Iskratsch T
    Developmental cell 44.3 (2018 Feb 5): 326-336.e3.

    Regional-specific alterations in cell-cell junctions, cytoskeletal networks and myosin-mediated mechanical cues coordinate collectivity of movement of epithelial cells in response to injury.
    Walker JL
    Experimental cell research 322.1 (2014 Mar 10): 133-48.

    A central role for vimentin in regulating repair function during healing of the lens epithelium.
    Walker JL
    Molecular biology of the cell 25.6 (2014 Mar): 776-90.

    Osmotic stress changes the expression and subcellular localization of the Batten disease protein CLN3.
    Pearce DA
    PloS one 8.6 (2013): e66203.

    Assembly of non-contractile dorsal stress fibers requires α-actinin-1 and Rac1 in migrating and spreading cells.
    Vallenius T
    Journal of cell science 126.Pt 1 (2013 Jan 1): 263-73.

    Nonmuscle myosin IIB links cytoskeleton to IRE1α signaling during ER stress.
    Qi L
    Developmental cell 23.6 (2012 Dec 11): 1141-52.

    WB References

    Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis.
    Conrad GW
    Cell motility and the cytoskeleton 19.3 (1991): 189-206.

    Differential localization of cytoplasmic myosin II isoforms A and B in avian interphase and dividing embryonic and immortalized cardiomyocytes and other cell types in vitro.
    Conrad GW
    Cell motility and the cytoskeleton 31.2 (1995): 93-112.

    Nonmuscle myosin promotes cytoplasmic localization of PBX.
    Featherstone M
    Molecular and cellular biology 23.10 (2003 May): 3636-45.

    Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
    Sheetz MP
    Biophysical journal 91.10 (2006 Nov 15): 3907-20.

    Isoform B of myosin II heavy chain mediates actomyosin contractility during TNFalpha-induced apoptosis.
    Vitale ML
    Journal of cell science 121.Pt 10 (2008 May 15): 1681-92.

    Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A.
    McCulloch CA
    Molecular biology of the cell 19.12 (2008 Dec): 5032-46.

    Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini.
    Jaffrey SR
    Proceedings of the National Academy of Sciences of the United States of America 106.46 (2009 Nov 17): 19310-5.

    Cholangiocyte myosin IIB is required for localized aggregation of sodium glucose cotransporter 1 to sites of Cryptosporidium parvum cellular invasion and facilitates parasite internalization.
    LaRusso NF
    Infection and immunity 78.7 (2010 Jul): 2927-36.

    An experimental model for studying the biomechanics of embryonic tendon: Evidence that the development of mechanical properties depends on the actinomyosin machinery.
    Kadler KE
    Matrix biology : journal of the International Society for Matrix Biology 29.8 (2010 Oct): 678-89.

    A novel interaction of CLN3 with nonmuscle myosin-IIB and defects in cell motility of Cln3(-/-) cells.
    Pearce DA
    Experimental cell research 317.1 (2011 Jan 1): 51-69.

    A Mec17-Myosin II Effector Axis Coordinates Microtubule Acetylation and Actin Dynamics to Control Primary Cilium Biogenesis.
    Yao TP
    PloS one 9.12 (2014): e114087.

    A central role for vimentin in regulating repair function during healing of the lens epithelium.
    Walker JL
    Molecular biology of the cell 25.6 (2014 Mar): 776-90.

    Gelsolin and non-muscle myosin IIA interact to mediate calcium-regulated collagen phagocytosis.
    McCulloch CA
    The Journal of biological chemistry 286.39 (2011 Sep 30): 34184-98.

    IHC References

    Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network.
    Hildebrand JD
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5191-203.

    Postnatal changes in the developing rat extraocular muscles.
    Andrade FH
    Investigative ophthalmology & visual science 52.7 (2011 Jun 7): 3962-9.

    IP References
    ELISA References
    All References

    Shroom regulates epithelial cell shape via the apical positioning of an actomyosin network.
    Hildebrand JD
    Journal of cell science 118.Pt 22 (2005 Nov 15): 5191-203.

    Postnatal changes in the developing rat extraocular muscles.
    Andrade FH
    Investigative ophthalmology & visual science 52.7 (2011 Jun 7): 3962-9.

    Subcellular compartmentalization of myosin isoforms in embryonic chick heart ventricle myocytes during cytokinesis.
    Conrad GW
    Cell motility and the cytoskeleton 19.3 (1991): 189-206.

    Differential localization of cytoplasmic myosin II isoforms A and B in avian interphase and dividing embryonic and immortalized cardiomyocytes and other cell types in vitro.
    Conrad GW
    Cell motility and the cytoskeleton 31.2 (1995): 93-112.

    Differential actin-dependent localization modulates the evolutionarily conserved activity of Shroom family proteins.
    Hildebrand JD
    The Journal of biological chemistry 281.29 (2006 Jul 21): 20542-54.

    Shroom4 (Kiaa1202) is an actin-associated protein implicated in cytoskeletal organization.
    Hildebrand JD
    Cell motility and the cytoskeleton 64.1 (2007 Jan): 49-63.

    Nonmuscle myosin IIA-dependent force inhibits cell spreading and drives F-actin flow.
    Sheetz MP
    Biophysical journal 91.10 (2006 Nov 15): 3907-20.

    Role of nonmuscle myosin IIB and N-RAP in cell spreading and myofibril assembly in primary mouse cardiomyocytes.
    Horowits R
    Cell motility and the cytoskeleton 65.9 (2008 Sep): 747-61.

    Spectral properties and mechanisms that underlie autofluorescent accumulations in Batten disease.
    Pearce DA
    Biochemical and biophysical research communications 382.2 (2009 May 1): 247-51.

    Cholangiocyte myosin IIB is required for localized aggregation of sodium glucose cotransporter 1 to sites of Cryptosporidium parvum cellular invasion and facilitates parasite internalization.
    LaRusso NF
    Infection and immunity 78.7 (2010 Jul): 2927-36.

    A novel interaction of CLN3 with nonmuscle myosin-IIB and defects in cell motility of Cln3(-/-) cells.
    Pearce DA
    Experimental cell research 317.1 (2011 Jan 1): 51-69.

    Cardiomyocytes Sense Matrix Rigidity through a Combination of Muscle and Non-muscle Myosin Contractions.
    Iskratsch T
    Developmental cell 44.3 (2018 Feb 5): 326-336.e3.

    Regional-specific alterations in cell-cell junctions, cytoskeletal networks and myosin-mediated mechanical cues coordinate collectivity of movement of epithelial cells in response to injury.
    Walker JL
    Experimental cell research 322.1 (2014 Mar 10): 133-48.

    A central role for vimentin in regulating repair function during healing of the lens epithelium.
    Walker JL
    Molecular biology of the cell 25.6 (2014 Mar): 776-90.

    Osmotic stress changes the expression and subcellular localization of the Batten disease protein CLN3.
    Pearce DA
    PloS one 8.6 (2013): e66203.

    Assembly of non-contractile dorsal stress fibers requires α-actinin-1 and Rac1 in migrating and spreading cells.
    Vallenius T
    Journal of cell science 126.Pt 1 (2013 Jan 1): 263-73.

    Nonmuscle myosin IIB links cytoskeleton to IRE1α signaling during ER stress.
    Qi L
    Developmental cell 23.6 (2012 Dec 11): 1141-52.

    Nonmuscle myosin promotes cytoplasmic localization of PBX.
    Featherstone M
    Molecular and cellular biology 23.10 (2003 May): 3636-45.

    Isoform B of myosin II heavy chain mediates actomyosin contractility during TNFalpha-induced apoptosis.
    Vitale ML
    Journal of cell science 121.Pt 10 (2008 May 15): 1681-92.

    Rap1 activation in collagen phagocytosis is dependent on nonmuscle myosin II-A.
    McCulloch CA
    Molecular biology of the cell 19.12 (2008 Dec): 5032-46.

    Global profiling of protease cleavage sites by chemoselective labeling of protein N-termini.
    Jaffrey SR
    Proceedings of the National Academy of Sciences of the United States of America 106.46 (2009 Nov 17): 19310-5.

    An experimental model for studying the biomechanics of embryonic tendon: Evidence that the development of mechanical properties depends on the actinomyosin machinery.
    Kadler KE
    Matrix biology : journal of the International Society for Matrix Biology 29.8 (2010 Oct): 678-89.

    A Mec17-Myosin II Effector Axis Coordinates Microtubule Acetylation and Actin Dynamics to Control Primary Cilium Biogenesis.
    Yao TP
    PloS one 9.12 (2014): e114087.

    Gelsolin and non-muscle myosin IIA interact to mediate calcium-regulated collagen phagocytosis.
    McCulloch CA
    The Journal of biological chemistry 286.39 (2011 Sep 30): 34184-98.

    Endogenous species of mammalian nonmuscle myosin IIA and IIB include activated monomers and heteropolymers.
    Svitkina T
    Current biology : CB 24.17 (2014 Sep 8): 1958-68.

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