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View product citations for antibody CeTAC1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: TAC-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Depositors Institution: Washington University Medical School
Host Species: mouse
Depositors Notes: Works on C. elegans whole mounts. Stains centrosomes.
Positive Tested Species Reactivity: C. elegans
Gene: tac-1
Antigen Molecular Weight: Predicted: 29 kDa; Apparent: 35 kDa
Alternate Gene Names: 2P40
Immunogen: Recombinant His6-tagged TAC-1 fusion protein
Clonality: Monoclonal
Alternate Antibody Name: TAC1, 4.5.6
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Immunogen Sequence: full length protein
Uniprot ID: G5ECG0 
Additional Information: The Transforming acid coiled-coil-containing protein 1, TAC-1, is a homolog of the transforming acid coiled coil protein family which localizes to centrosomes in a cell cycle dependent manner.
Entrez Gene ID: 175133 
Antibody Registry ID: AB_10661034 
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
CeTAC1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product CeTAC1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References
    All References

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