LMN1

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$40.00
SKU: LMN1
View product citations for antibody LMN1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Lamin-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgM, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans, Mouse
Depositors Notes: Works well on C. elegans whole mounts. Stains nuclear membranes.
Antigen Molecular Weight: Predicted: 64 kDa; Apparent: 73 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: lmn-1
Immunogen: Recombinant His6-tagged LMN-1 (aa 217-550) fusion protein
Alternate Gene Names: lam-1
Alternate Antibody Name: 2G1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 217-550
Uniprot ID: Q21443 
Immunogen Sequence: Partial protein
Entrez Gene ID: 172687 
Additional Characterization:  
Antibody Registry ID: AB_10573809 
Additional Information: Lamin is a structural nuclear envelope protein of the intermediate filament super family that associates with the nuclear membrane.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LMN1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product LMN1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

1 Reference

  • Initial Publication
  • IF References
  • WB References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

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