Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgM, kappa light chain
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans, Mouse
Depositors Notes: Works well on C. elegans whole mounts. Stains nuclear membranes.
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged LMN-1 (aa 217-550) fusion protein
Alternate Gene Names: lam-1
Alternate Antibody Name: 2G1
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 217-550
Uniprot ID: Q21443
Immunogen Sequence: Partial protein
Entrez Gene ID: 172687
Antibody Registry ID: AB_10573809
Additional Information: Lamin is a structural nuclear envelope protein of the intermediate filament super family that associates with the nuclear membrane.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
LMN1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product LMN1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Epitope Map References