EAP3

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$40.00
SKU: EAP3-s
View product citations for antibody EAP3 on CiteAb

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Available: 30

DSHB Data Sheet

Catalog Fields

Antigen: Transitin
Hybridoma Cells Available: No
Antigen Species: Chick
Depositor: Cole, G.J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: North Carolina Central University, Durham
Positive Tested Species Reactivity: Chick
Depositors Notes: EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Antigen Molecular Weight: 300 kDa apparent/ 202 kDa derived
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: Purified transitin from E9 brain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID: Q90831 
Immunogen Sequence: Full length sequence
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_2282449 
Additional Information: RRID:AB_2282449
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EAP3 was deposited to the DSHB by Cole, G.J. (DSHB Hybridoma Product EAP3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
    Cole GJ
    Molecular and cellular neurosciences 10.1-2 (1997): 71-86.

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
    Osumi N
    Development (Cambridge, England) 134.13 (2007 Jul): 2425-33.

    Regulation of neuronal and photoreceptor cell differentiation by Wnt signaling from iris-derived stem/progenitor cells of the chick in flat vs. Matrigel-embedding cultures.
    Araki M
    Brain research 1704. (2019 Feb 1): 207-218.

    Notch signaling influences neuroprotective and proliferative properties of mature Müller glia.
    Fischer AJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.8 (2010 Feb 24): 3101-12.

    The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Fischer AJ
    PloS one 7.9 (2012): e44477.

    Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina.
    Ventura AL
    PloS one 8.1 (2013): e53517.

    Reactive retinal microglia, neuronal survival, and the formation of retinal folds and detachments.
    Milani-Nejad N
    Glia 63.2 (2015 Feb): 313-27.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity.
    Fischer AJ
    Experimental neurology 273. (2015 Nov): 114-25.

    WB References

    Avian transitin expression mirrors glial cell fate restrictions during neural crest development.
    Weston JA
    Developmental dynamics : an official publication of the American Association of Anatomists 218.1 (2000 May): 150-9.

    IHC References

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Glial migratory streams in the developing hindbrain: a slice culture approach.
    Domowicz MS
    Journal of neuroscience methods 177.1 (2009 Feb 15): 30-43.

    Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.
    Price SR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.2 (2012 Jan 11): 490-505.

    A chick model of retinal detachment: cone rich and novel.
    Fischer AJ
    PloS one 7.9 (2012): e44257.

    A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.
    Sela-Donenfeld D
    BMC biology 14. (2016 Jul 8): 57.

    Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina.
    Fischer AJ
    Scientific reports 6. (2016 Oct 19): 35703.

    All References

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Glial migratory streams in the developing hindbrain: a slice culture approach.
    Domowicz MS
    Journal of neuroscience methods 177.1 (2009 Feb 15): 30-43.

    Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.
    Price SR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.2 (2012 Jan 11): 490-505.

    A chick model of retinal detachment: cone rich and novel.
    Fischer AJ
    PloS one 7.9 (2012): e44257.

    A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.
    Sela-Donenfeld D
    BMC biology 14. (2016 Jul 8): 57.

    Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina.
    Fischer AJ
    Scientific reports 6. (2016 Oct 19): 35703.

    Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
    Cole GJ
    Molecular and cellular neurosciences 10.1-2 (1997): 71-86.

    Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
    Osumi N
    Development (Cambridge, England) 134.13 (2007 Jul): 2425-33.

    Regulation of neuronal and photoreceptor cell differentiation by Wnt signaling from iris-derived stem/progenitor cells of the chick in flat vs. Matrigel-embedding cultures.
    Araki M
    Brain research 1704. (2019 Feb 1): 207-218.

    Notch signaling influences neuroprotective and proliferative properties of mature Müller glia.
    Fischer AJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.8 (2010 Feb 24): 3101-12.

    The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Fischer AJ
    PloS one 7.9 (2012): e44477.

    Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina.
    Ventura AL
    PloS one 8.1 (2013): e53517.

    Reactive retinal microglia, neuronal survival, and the formation of retinal folds and detachments.
    Milani-Nejad N
    Glia 63.2 (2015 Feb): 313-27.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity.
    Fischer AJ
    Experimental neurology 273. (2015 Nov): 114-25.

    Avian transitin expression mirrors glial cell fate restrictions during neural crest development.
    Weston JA
    Developmental dynamics : an official publication of the American Association of Anatomists 218.1 (2000 May): 150-9.

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