EAP3

(0)No Reviews yet
$50.00
QuantityPrice
1 – 19$50.00
20 – 49$35.00
50 – 99$32.00
100 +$30.00
SKU: EAP3-s

In Stock

Available: 12

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: EAP3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol:
Ab Isotype: MIgG1, kappa light chain
Gene Name:
Antibody Registry ID: AB_2282449 
Uniprot ID: Q90831 
RRID:  
Entrez Gene ID:  
Clonality: Monoclonal
Immunogen: Purified transitin from E9 brain
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Sp2/0
Epitope Mapped: No
Antigen Name: Transitin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 9/25/2003
Antigen Molecular Weight: 300 kDa apparent/ 202 kDa derived
Depositor: Cole, G.J.
Antigen Sequence:
Depositor Institution: North Carolina Central University, Durham
Antigen Species: Chick
Depositor Notes: EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Chick
Additional Information: RRID:AB_2282449
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EAP3 was deposited to the DSHB by Cole, G.J. (DSHB Hybridoma Product EAP3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

17 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication
    IF References

    Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
    Cole GJ
    Molecular and cellular neurosciences 10.1-2 (1997): 71-86.

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
    Osumi N
    Development (Cambridge, England) 134.13 (2007 Jul): 2425-33.

    Regulation of neuronal and photoreceptor cell differentiation by Wnt signaling from iris-derived stem/progenitor cells of the chick in flat vs. Matrigel-embedding cultures.
    Araki M
    Brain research 1704. (2019 Feb 1): 207-218.

    Notch signaling influences neuroprotective and proliferative properties of mature Müller glia.
    Fischer AJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.8 (2010 Feb 24): 3101-12.

    The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Fischer AJ
    PloS one 7.9 (2012): e44477.

    Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina.
    Ventura AL
    PloS one 8.1 (2013): e53517.

    Reactive retinal microglia, neuronal survival, and the formation of retinal folds and detachments.
    Milani-Nejad N
    Glia 63.2 (2015 Feb): 313-27.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity.
    Fischer AJ
    Experimental neurology 273. (2015 Nov): 114-25.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

    WB References

    Avian transitin expression mirrors glial cell fate restrictions during neural crest development.
    Weston JA
    Developmental dynamics : an official publication of the American Association of Anatomists 218.1 (2000 May): 150-9.

    IHC References

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Glial migratory streams in the developing hindbrain: a slice culture approach.
    Domowicz MS
    Journal of neuroscience methods 177.1 (2009 Feb 15): 30-43.

    Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.
    Price SR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.2 (2012 Jan 11): 490-505.

    A chick model of retinal detachment: cone rich and novel.
    Fischer AJ
    PloS one 7.9 (2012): e44257.

    A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.
    Sela-Donenfeld D
    BMC biology 14. (2016 Jul 8): 57.

    Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina.
    Fischer AJ
    Scientific reports 6. (2016 Oct 19): 35703.

    All References

    Immunocytochemical localization of a novel radial glial intermediate filament protein.
    Lee JA
    Brain research. Developmental brain research 101.1-2 (1997 Jul 18): 225-38.

    Glial migratory streams in the developing hindbrain: a slice culture approach.
    Domowicz MS
    Journal of neuroscience methods 177.1 (2009 Feb 15): 30-43.

    Catenin-dependent cadherin function drives divisional segregation of spinal motor neurons.
    Price SR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.2 (2012 Jan 11): 490-505.

    A chick model of retinal detachment: cone rich and novel.
    Fischer AJ
    PloS one 7.9 (2012): e44257.

    A new role of hindbrain boundaries as pools of neural stem/progenitor cells regulated by Sox2.
    Sela-Donenfeld D
    BMC biology 14. (2016 Jul 8): 57.

    Jak/Stat signaling regulates the proliferation and neurogenic potential of Müller glia-derived progenitor cells in the avian retina.
    Fischer AJ
    Scientific reports 6. (2016 Oct 19): 35703.

    Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
    Cole GJ
    Molecular and cellular neurosciences 10.1-2 (1997): 71-86.

    Transitin, a nestin-like intermediate filament protein, mediates cortical localization and the lateral transport of Numb in mitotic avian neuroepithelial cells.
    Osumi N
    Development (Cambridge, England) 134.13 (2007 Jul): 2425-33.

    Regulation of neuronal and photoreceptor cell differentiation by Wnt signaling from iris-derived stem/progenitor cells of the chick in flat vs. Matrigel-embedding cultures.
    Araki M
    Brain research 1704. (2019 Feb 1): 207-218.

    Notch signaling influences neuroprotective and proliferative properties of mature Müller glia.
    Fischer AJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.8 (2010 Feb 24): 3101-12.

    The reactivity, distribution and abundance of Non-astrocytic Inner Retinal Glial (NIRG) cells are regulated by microglia, acute damage, and IGF1.
    Fischer AJ
    PloS one 7.9 (2012): e44477.

    Inhibition of PI3K/Akt pathway impairs G2/M transition of cell cycle in late developing progenitors of the avian embryo retina.
    Ventura AL
    PloS one 8.1 (2013): e53517.

    Reactive retinal microglia, neuronal survival, and the formation of retinal folds and detachments.
    Milani-Nejad N
    Glia 63.2 (2015 Feb): 313-27.

    Involvement of nucleotides in glial growth following scratch injury in avian retinal cell monolayer cultures.
    Ventura AL
    Purinergic signalling 11.2 (2015 Jun): 183-201.

    Activation of glucocorticoid receptors in Müller glia is protective to retinal neurons and suppresses microglial reactivity.
    Fischer AJ
    Experimental neurology 273. (2015 Nov): 114-25.

    Neurospheres obtained from the ciliary margin of the chicken eye possess positional values and retinal ganglion cells differentiated from them respond to EphA/ephrin-A system.
    Scicolone G
    Experimental eye research 217. (2022 Apr): 108965.

    Avian transitin expression mirrors glial cell fate restrictions during neural crest development.
    Weston JA
    Developmental dynamics : an official publication of the American Association of Anatomists 218.1 (2000 May): 150-9.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review