EAP3
Catalog Fields
Antigen: Transitin
Hybridoma Cells Available: No
Antigen Species: Chick
Depositor: Cole, G.J.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: North Carolina Central University, Durham
Positive Tested Species Reactivity: Chick
Depositors Notes: EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Predicted Species Reactivity:
Gene:
Immunogen: Purified transitin from E9 brain
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: Sp2/0
Epitope Location or Sequence:
Uniprot ID: Q90831
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_2282449
Additional Information: RRID:AB_2282449
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EAP3 was deposited to the DSHB by Cole, G.J. (DSHB Hybridoma Product EAP3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
16 References
Initial Publication
Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
Cole GJ
Molecular and cellular neurosciences 10.1-2 (1997): 71-86.