EAP3
Catalog Fields
Antigen: Transitin
Available to For-Profits: Yes
Antigen Species: Chick
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1, kappa light chain
Depositor: Cole, G.J.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Chick
Depositors Institution: North Carolina Central University, Durham
Antigen Molecular Weight: 300 kDa apparent/ 202 kDa derived
Depositors Notes: EAP3 labels the intermediate filament network in radial glia and myoblasts. This antigen is also known as EAP-300.
Immunogen: Purified transitin from E9 brain
Gene:
Alternate Antibody Name:
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: No
Uniprot ID: Q90831
Epitope Location or Sequence:
Antibody Registry ID: AB_2282449
Recommended Applications: Immunofluorescence, Immunohistochemistry
Additional Information: RRID:AB_2282449
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
EAP3 was deposited to the DSHB by Cole, G.J. (DSHB Hybridoma Product EAP3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
17 References
Initial Publication
Molecular cloning of a new intermediate filament protein expressed by radial glia and demonstration of alternative splicing in a novel heptad repeat region located in the carboxy-terminal tail domain.
Cole GJ
Molecular and cellular neurosciences 10.1-2 (1997): 71-86.