PAX6

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$40.00
SKU: PAX6
View product citations for antibody PAX6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Pax6
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Kawakami, A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Tokyo Institute of Technology
Positive Tested Species Reactivity: Amphibian, Avian, Fish, Human, Lizard, Mouse, Opossum, Planaria, Rat, Turtle, Zebrafish
Depositors Notes: This antibody recognizes a transcription factor important in neurodevelopment and differentiation of islet cells in the pancreas.
Antigen Molecular Weight: Predicted: 48 kDa; Apparent: 38, 40 and 46, 48 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Pax-6
Immunogen: Recombinant partial protein (N-terminal region, aa 1-223)
Alternate Gene Names: PAX6
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3U1
Epitope Location or Sequence: N-terminal region, aa 1-223
Uniprot ID: O42348 
Immunogen Sequence: Partial protein
Entrez Gene ID: 395943 
Additional Characterization:
Antibody Registry ID: AB_528427 
Additional Information:
Recommended Applications: Chromatin Immunoprecipitation, FACS, FFPE, Function Blocking, Gel Supershift, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PAX6 was deposited to the DSHB by Kawakami, A. (DSHB Hybridoma Product PAX6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

58 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FFPE References
  • FB References
  • FACS References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response.
    Pan G
    Science China. Life sciences 57.2 (2014 Feb): 162-70.

    Ikaros promotes early-born neuronal fates in the cerebral cortex.
    Livesey FJ
    Proceedings of the National Academy of Sciences of the United States of America 110.8 (2013 Feb 19): E716-25.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Generation of induced pluripotent stem cells from mouse cancer cells.
    Chui YL
    Cancer biotherapy and radiopharmaceuticals 27.10 (2012 Dec): 694-700.

    β-Catenin signaling specifies progenitor cell identity in parallel with Shh signaling in the developing mammalian thalamus.
    Nakagawa Y
    Development (Cambridge, England) 139.15 (2012 Aug): 2692-702.

    Activation of the hedgehog signaling pathway in the developing lens stimulates ectopic FoxE3 expression and disruption in fiber cell differentiation.
    West-Mays JA
    Investigative ophthalmology and visual science 53.7 (2012 Jun 5): 3316-30.

    Guiding the morphogenesis of dissociated newborn mouse retinal cells and hES cell-derived retinal cells by soft lithography-patterned microchannel PLGA scaffolds.
    Reh TA
    Biomaterials 33.5 (2012 Feb): 1396-405.

    Antagonistic and cooperative actions of Kif7 and Sufu define graded intracellular Gli activities in Hedgehog signaling.
    Hui CC
    PloS one 7.11 (2012): e50193.

    Negative regulation of Yap during neuronal differentiation.
    Turner DL
    Developmental biology 361.1 (2012 Jan 1): 103-15.

    VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm.
    Ruhrberg C
    Neuron 70.5 (2011 Jun 9): 951-65.

    Geminin regulates cortical progenitor proliferation and differentiation.
    Taraviras S
    Stem cells (Dayton, Ohio) 29.8 (2011 Aug): 1269-82.

    Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.
    Schwob JE
    Cell stem cell 21.6 (2017 Dec 7): 761-774.e5.

    Genetically unmatched human iPSC and ESC exhibit equivalent gene expression and neuronal differentiation potential.
    Hasan A
    Scientific reports 7.1 (2017 Dec 13): 17504.

    The Role of Sonic Hedgehog in the Specification of Human Cortical Progenitors In Vitro.
    Zecevic N
    Cerebral cortex (New York, N.Y. : 1991) 26.1 (2016 Jan): 131-43.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    WB References

    Defined extracellular matrix components are necessary for definitive endoderm induction.
    Engler AJ
    Stem cells (Dayton, Ohio) 31.10 (2013 Oct): 2084-94.

    miR-290 cluster modulates pluripotency by repressing canonical NF-κB signaling.
    Kaltschmidt C
    Stem cells (Dayton, Ohio) 30.4 (2012 Apr): 655-64.

    Loss of Shp2-mediated mitogen-activated protein kinase signaling in Muller glial cells results in retinal degeneration.
    Zhang X
    Molecular and cellular biology 31.14 (2011 Jul): 2973-83.

    Lens induction requires attenuation of ERK signaling by Nf1.
    Zhang X
    Human molecular genetics 20.7 (2011 Apr 1): 1315-23.

    Agitation can induce differentiation of human pluripotent stem cells in microcarrier cultures.
    Oh SK
    Tissue engineering. Part C, Methods 17.2 (2011 Feb): 165-72.

    Selective expansion of the beta-cell compartment in the pancreas of keratinocyte growth factor transgenic mice.
    Schmid RM
    American journal of physiology. Gastrointestinal and liver physiology 294.5 (2008 May): G1139-47.

    Pax6 overexpression suppresses cell proliferation and retards the cell cycle in corneal epithelial cells.
    Fini ME
    Investigative ophthalmology and visual science 47.6 (2006 Jun): 2397-407.

    FKBP8 is a negative regulator of mouse sonic hedgehog signaling in neural tissues.
    Li T
    Development (Cambridge, England) 131.9 (2004 May): 2149-59.

    Acetylation of the BETA2 transcription factor by p300-associated factor is important in insulin gene expression.
    Stein R
    The Journal of biological chemistry 279.11 (2004 Mar 12): 9796-802.

    Brn-4 transcription factor expression targeted to the early developing mouse pancreas induces ectopic glucagon gene expression in insulin-producing beta cells.
    Habener JF
    The Journal of biological chemistry 277.18 (2002 May 3): 16028-32.

    IHC References

    Immunohistochemical analysis of Pax6 and Pax7 expression in the CNS of adult Xenopus laevis.
    González A
    Journal of chemical neuroanatomy 57-58. (2014 May): 24-41.

    Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation.
    Radtke F
    Pigment cell and melanoma research 27.4 (2014 Jul): 580-9.

    Utility of PAX8 mouse monoclonal antibody in the diagnosis of thyroid, thymic, pleural and lung tumours: a comparison with polyclonal PAX8 antibody.
    Tsuta K
    Histopathology 65.4 (2014 Oct): 465-72.

    Scratch2 modulates neurogenesis and cell migration through antagonism of bHLH proteins in the developing neocortex.
    Stoykova A
    Cerebral cortex (New York, N.Y. : 1991) 24.3 (2014 Mar): 754-72.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    Enhanced tissue differentiation in the developing mouse brain using magnetic resonance micro-histology.
    Lythgoe MF
    Magnetic resonance in medicine 70.5 (2013 Nov): 1380-8.

    ARX regulates cortical intermediate progenitor cell expansion and upper layer neuron formation through repression of Cdkn1c.
    Broccoli V
    Cerebral cortex (New York, N.Y. : 1991) 25.2 (2015 Feb): 322-35.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells.
    Greer CA
    Molecular and cellular neurosciences 54. (2013 May): 58-70.

    Geminin regulates cortical progenitor proliferation and differentiation.
    Taraviras S
    Stem cells (Dayton, Ohio) 29.8 (2011 Aug): 1269-82.

    Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
    Volovitch M
    Neural development 2. (2007 Jan 17): 2.

    Stress-induced unfolded protein response contributes to Zika virus-associated microcephaly.
    Nguyen L
    Nature neuroscience 21.1 (2018 Jan): 63-71.

    Zika Virus Can Strongly Infect and Disrupt Secondary Organizers in the Ventricular Zone of the Embryonic Chicken Brain.
    Fekete DM
    Cell reports 23.3 (2018 Apr 17): 692-700.

    Bhlhb5::flpo allele uncovers a requirement for Bhlhb5 for the development of the dorsal cochlear nucleus.
    Ross SE
    Developmental biology 414.2 (2016 Jun 15): 149-60.

    GemC1 is a critical switch for neural stem cell generation in the postnatal brain.
    Taraviras S
    Glia 67.12 (2019 Dec): 2360-2373.

    Early expression of Tubulin Beta-III in avian cranial neural crest cells.
    Rogers CD
    Gene expression patterns : GEP 34. (2019 Dec): 119067.

    Expression of Lhx6 in the adult and developing mouse retina.
    Gan L
    Gene expression patterns : GEP 34. (2019 Dec): 119065.

    IP References

    Acetylation of the BETA2 transcription factor by p300-associated factor is important in insulin gene expression.
    Stein R
    The Journal of biological chemistry 279.11 (2004 Mar 12): 9796-802.

    FFPE References

    Expression pattern of cLhx2b, cZic1 and cZic3 in the developing chick diencephalon.
    Golden JA
    Mechanisms of development 115.1-2 (2002 Jul): 147-50.

    Pax6 is misexpressed in Sox1 null lens fiber cells.
    Maas RL
    Gene expression patterns : GEP 7.5 (2007 Apr): 606-13.

    The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo.
    West JD
    Developmental biology 255.2 (2003 Mar 15): 303-12.

    GemC1 is a critical switch for neural stem cell generation in the postnatal brain.
    Taraviras S
    Glia 67.12 (2019 Dec): 2360-2373.

    FB References

    Paracrine Pax6 activity regulates oligodendrocyte precursor cell migration in the chick embryonic neural tube.
    Prochiantz A
    Development (Cambridge, England) 138.22 (2011 Nov): 4991-5001.

    FACS References
    Epitope Map References
    Gel Supershift References

    Regulation of the FABP7 gene by PAX6 in malignant glioma cells.
    Godbout R
    Biochemical and biophysical research communications 422.3 (2012 Jun 8): 482-7.

    The activation of the rat insulin gene II by BETA2 and PDX-1 in rat insulinoma cells is repressed by Pax6.
    Walther R
    Molecular endocrinology (Baltimore, Md.) 24.12 (2010 Dec): 2331-42.

    All References

    Immunohistochemical analysis of Pax6 and Pax7 expression in the CNS of adult Xenopus laevis.
    González A
    Journal of chemical neuroanatomy 57-58. (2014 May): 24-41.

    Notch signaling in the pigmented epithelium of the anterior eye segment promotes ciliary body development at the expense of iris formation.
    Radtke F
    Pigment cell and melanoma research 27.4 (2014 Jul): 580-9.

    Utility of PAX8 mouse monoclonal antibody in the diagnosis of thyroid, thymic, pleural and lung tumours: a comparison with polyclonal PAX8 antibody.
    Tsuta K
    Histopathology 65.4 (2014 Oct): 465-72.

    Scratch2 modulates neurogenesis and cell migration through antagonism of bHLH proteins in the developing neocortex.
    Stoykova A
    Cerebral cortex (New York, N.Y. : 1991) 24.3 (2014 Mar): 754-72.

    ADAM10 negatively regulates neuronal differentiation during spinal cord development.
    Luo J
    PloS one 9.1 (2014): e84617.

    Enhanced tissue differentiation in the developing mouse brain using magnetic resonance micro-histology.
    Lythgoe MF
    Magnetic resonance in medicine 70.5 (2013 Nov): 1380-8.

    ARX regulates cortical intermediate progenitor cell expansion and upper layer neuron formation through repression of Cdkn1c.
    Broccoli V
    Cerebral cortex (New York, N.Y. : 1991) 25.2 (2015 Feb): 322-35.

    Neuronal intermediate filament α-internexin is expressed by neuronal lineages in the developing chicken retina.
    Chien CL
    Experimental eye research 110. (2013 May): 18-25.

    Pax6 regulates Tbr1 and Tbr2 expressions in olfactory bulb mitral cells.
    Greer CA
    Molecular and cellular neurosciences 54. (2013 May): 58-70.

    Geminin regulates cortical progenitor proliferation and differentiation.
    Taraviras S
    Stem cells (Dayton, Ohio) 29.8 (2011 Aug): 1269-82.

    Direct non-cell autonomous Pax6 activity regulates eye development in the zebrafish.
    Volovitch M
    Neural development 2. (2007 Jan 17): 2.

    Stress-induced unfolded protein response contributes to Zika virus-associated microcephaly.
    Nguyen L
    Nature neuroscience 21.1 (2018 Jan): 63-71.

    Zika Virus Can Strongly Infect and Disrupt Secondary Organizers in the Ventricular Zone of the Embryonic Chicken Brain.
    Fekete DM
    Cell reports 23.3 (2018 Apr 17): 692-700.

    Bhlhb5::flpo allele uncovers a requirement for Bhlhb5 for the development of the dorsal cochlear nucleus.
    Ross SE
    Developmental biology 414.2 (2016 Jun 15): 149-60.

    GemC1 is a critical switch for neural stem cell generation in the postnatal brain.
    Taraviras S
    Glia 67.12 (2019 Dec): 2360-2373.

    Early expression of Tubulin Beta-III in avian cranial neural crest cells.
    Rogers CD
    Gene expression patterns : GEP 34. (2019 Dec): 119067.

    Expression of Lhx6 in the adult and developing mouse retina.
    Gan L
    Gene expression patterns : GEP 34. (2019 Dec): 119065.

    Neural progenitor cells from human induced pluripotent stem cells generated less autogenous immune response.
    Pan G
    Science China. Life sciences 57.2 (2014 Feb): 162-70.

    Ikaros promotes early-born neuronal fates in the cerebral cortex.
    Livesey FJ
    Proceedings of the National Academy of Sciences of the United States of America 110.8 (2013 Feb 19): E716-25.

    Rbfox3-regulated alternative splicing of Numb promotes neuronal differentiation during development.
    Kawamoto S
    The Journal of cell biology 200.4 (2013 Feb 18): 443-58.

    Generation of induced pluripotent stem cells from mouse cancer cells.
    Chui YL
    Cancer biotherapy and radiopharmaceuticals 27.10 (2012 Dec): 694-700.

    β-Catenin signaling specifies progenitor cell identity in parallel with Shh signaling in the developing mammalian thalamus.
    Nakagawa Y
    Development (Cambridge, England) 139.15 (2012 Aug): 2692-702.

    Activation of the hedgehog signaling pathway in the developing lens stimulates ectopic FoxE3 expression and disruption in fiber cell differentiation.
    West-Mays JA
    Investigative ophthalmology and visual science 53.7 (2012 Jun 5): 3316-30.

    Guiding the morphogenesis of dissociated newborn mouse retinal cells and hES cell-derived retinal cells by soft lithography-patterned microchannel PLGA scaffolds.
    Reh TA
    Biomaterials 33.5 (2012 Feb): 1396-405.

    Antagonistic and cooperative actions of Kif7 and Sufu define graded intracellular Gli activities in Hedgehog signaling.
    Hui CC
    PloS one 7.11 (2012): e50193.

    Negative regulation of Yap during neuronal differentiation.
    Turner DL
    Developmental biology 361.1 (2012 Jan 1): 103-15.

    VEGF signaling through neuropilin 1 guides commissural axon crossing at the optic chiasm.
    Ruhrberg C
    Neuron 70.5 (2011 Jun 9): 951-65.

    Three Tctn proteins are functionally conserved in the regulation of neural tube patterning and Gli3 processing but not ciliogenesis and Hedgehog signaling in the mouse.
    Wang B
    Developmental biology 430.1 (2017 Oct 1): 156-165.

    Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.
    Schwob JE
    Cell stem cell 21.6 (2017 Dec 7): 761-774.e5.

    Genetically unmatched human iPSC and ESC exhibit equivalent gene expression and neuronal differentiation potential.
    Hasan A
    Scientific reports 7.1 (2017 Dec 13): 17504.

    The Role of Sonic Hedgehog in the Specification of Human Cortical Progenitors In Vitro.
    Zecevic N
    Cerebral cortex (New York, N.Y. : 1991) 26.1 (2016 Jan): 131-43.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Identification and characterization of early photoreceptor cis-regulatory elements and their relation to Onecut1.
    Emerson MM
    Neural development 13.1 (2018 Nov 22): 26.

    TrxG Complex Catalytic and Non-catalytic Activity Play Distinct Roles in Pancreas Progenitor Specification and Differentiation.
    Hoffman BG
    Cell reports 28.7 (2019 Aug 13): 1830-1844.e6.

    Defined extracellular matrix components are necessary for definitive endoderm induction.
    Engler AJ
    Stem cells (Dayton, Ohio) 31.10 (2013 Oct): 2084-94.

    miR-290 cluster modulates pluripotency by repressing canonical NF-κB signaling.
    Kaltschmidt C
    Stem cells (Dayton, Ohio) 30.4 (2012 Apr): 655-64.

    Loss of Shp2-mediated mitogen-activated protein kinase signaling in Muller glial cells results in retinal degeneration.
    Zhang X
    Molecular and cellular biology 31.14 (2011 Jul): 2973-83.

    Lens induction requires attenuation of ERK signaling by Nf1.
    Zhang X
    Human molecular genetics 20.7 (2011 Apr 1): 1315-23.

    Agitation can induce differentiation of human pluripotent stem cells in microcarrier cultures.
    Oh SK
    Tissue engineering. Part C, Methods 17.2 (2011 Feb): 165-72.

    Selective expansion of the beta-cell compartment in the pancreas of keratinocyte growth factor transgenic mice.
    Schmid RM
    American journal of physiology. Gastrointestinal and liver physiology 294.5 (2008 May): G1139-47.

    Pax6 overexpression suppresses cell proliferation and retards the cell cycle in corneal epithelial cells.
    Fini ME
    Investigative ophthalmology and visual science 47.6 (2006 Jun): 2397-407.

    FKBP8 is a negative regulator of mouse sonic hedgehog signaling in neural tissues.
    Li T
    Development (Cambridge, England) 131.9 (2004 May): 2149-59.

    Acetylation of the BETA2 transcription factor by p300-associated factor is important in insulin gene expression.
    Stein R
    The Journal of biological chemistry 279.11 (2004 Mar 12): 9796-802.

    Brn-4 transcription factor expression targeted to the early developing mouse pancreas induces ectopic glucagon gene expression in insulin-producing beta cells.
    Habener JF
    The Journal of biological chemistry 277.18 (2002 May 3): 16028-32.

    Expression pattern of cLhx2b, cZic1 and cZic3 in the developing chick diencephalon.
    Golden JA
    Mechanisms of development 115.1-2 (2002 Jul): 147-50.

    Pax6 is misexpressed in Sox1 null lens fiber cells.
    Maas RL
    Gene expression patterns : GEP 7.5 (2007 Apr): 606-13.

    The roles of Pax6 in the cornea, retina, and olfactory epithelium of the developing mouse embryo.
    West JD
    Developmental biology 255.2 (2003 Mar 15): 303-12.

    Paracrine Pax6 activity regulates oligodendrocyte precursor cell migration in the chick embryonic neural tube.
    Prochiantz A
    Development (Cambridge, England) 138.22 (2011 Nov): 4991-5001.

    Chromatin insulator elements block transgene silencing in engineered human embryonic stem cell lines at a defined chromosome 13 locus.
    Liu Y
    Stem cells and development 21.2 (2012 Jan 20): 191-205.

    Novel lines of Pax6-/- embryonic stem cells exhibit reduced neurogenic capacity without loss of viability.
    Price DJ
    BMC neuroscience 11. (2010 Feb 24): 26.

    LHX2 regulates the neural differentiation of human embryonic stem cells via transcriptional modulation of PAX6 and CER1.
    Kuo HC
    Nucleic acids research 41.16 (2013 Sep): 7753-70.

    Fibroblast growth factor regulates human neuroectoderm specification through ERK1/2-PARP-1 pathway.
    Zhang SC
    Stem cells (Dayton, Ohio) 29.12 (2011 Dec): 1975-82.

    Epigenetic regulation of miR-302 by JMJD1C inhibits neural differentiation of human embryonic stem cells.
    Xu RH
    The Journal of biological chemistry 289.4 (2014 Jan 24): 2384-95.

    Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling.
    Briscoe J
    Cell 90.1 (1997 Jul 11): 169-80.

    Distributions of PAX6 and PAX7 proteins suggest their involvement in both early and late phases of chick brain development.
    Fujisawa H
    Mechanisms of development 66.1-2 (1997 Aug): 119-30.

    Regulation of the FABP7 gene by PAX6 in malignant glioma cells.
    Godbout R
    Biochemical and biophysical research communications 422.3 (2012 Jun 8): 482-7.

    The activation of the rat insulin gene II by BETA2 and PDX-1 in rat insulinoma cells is repressed by Pax6.
    Walther R
    Molecular endocrinology (Baltimore, Md.) 24.12 (2010 Dec): 2331-42.

    Small-molecule induction promotes corneal epithelial cell differentiation from human induced pluripotent stem cells.
    Skottman H
    Stem cell reports 2.2 (2014 Feb 11): 219-31.

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