Catalog Fields
Antigen: TGFbeta3, latent, precursor domain
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Runyan, R.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Arizona
Positive Tested Species Reactivity: Chicken, Planaria
Depositors Notes: Stains chick embryo heart.
Antigen Molecular Weight: Predicted: 45 kDa, 32 kDa (latent peptide), 13 kDa (TGFbate3 active peptide)
Predicted Species Reactivity:
Gene: TGFB3
Immunogen: Recombinant GST/TGFbeta3 fusion protein (one of three boosts with active domain of TGFbeta3)
Alternate Gene Names: TGF-beta3
Alternate Antibody Name: G-B3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Latency-associated peptide
Uniprot ID: P16047
Immunogen Sequence: Full length protein
Entrez Gene ID: 396438
Antibody Registry ID: AB_531802
Additional Information:
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G-B3(lat) was deposited to the DSHB by Runyan, R. (DSHB Hybridoma Product G-B3(lat))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
TGFbeta2 and TGFbeta3 have separate and sequential activities during epithelial-mesenchymal cell transformation in the embryonic heart.
Runyan RB
Developmental biology 208.2 (1999 Apr 15): 530-45.