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SKU: G-B3(lat)
View product citations for antibody G-B3(lat) on CiteAb

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Available: 35

DSHB Data Sheet

Catalog Fields

Antigen: TGFbeta3, latent, precursor domain
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Runyan, R.
Isotype: MIgM
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Cell Biology and Anatomy, University of Arizona
Positive Tested Species Reactivity: Chicken, Planaria
Depositors Notes: Stains chick embryo heart.
Antigen Molecular Weight: Predicted: 45 kDa, 32 kDa (latent peptide), 13 kDa (TGFbate3 active peptide)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: TGFB3
Immunogen: Recombinant GST/TGFbeta3 fusion protein (one of three boosts with active domain of TGFbeta3)
Alternate Gene Names: TGF-beta3
Alternate Antibody Name: G-B3
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1
Epitope Location or Sequence: Latency-associated peptide
Uniprot ID: P16047 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396438 
Additional Characterization:  
Antibody Registry ID: AB_531802 
Additional Information:
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
G-B3(lat) was deposited to the DSHB by Runyan, R. (DSHB Hybridoma Product G-B3(lat))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • WB References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    ELISA References
    FFPE References
    FB References
    Epitope Map References
    All References

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