Product Name/ID: HCP4
Available to For-Profits: Yes
Alternate Antibody Name: 2E2
Gene Name: hcp-4
Ab Isotype: MIgM
Entrez Gene ID: 171964
Immunogen: Recombinant His6-tagged HCP-4 (N-terminal, aa 1-210) fusion protein
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: HCP-4 (CENP-C)
Epitope Location or Sequence: N-terminal, aa 1-210
Alternate Antigen Name:
Deposit Date: 2/16/2010
Antigen Molecular Weight: Predicted: 97 kDa; apparent: 80 and 125 kDa
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Works well on C. elegans whole mounts. Stains the centromeres (nuclei and chromosomes). Works poorly on immunoblots.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: The CENP-C-like protein HCP-4 is a homolog of CENP-C which localizes to the centromere of chromosomes.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HCP4 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product HCP4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Epitope Map References