Antigen: HCP-4 (CENP-C)
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Works well on C. elegans whole mounts. Stains the centromeres (nuclei and chromosomes). Works poorly on immunoblots.
Antigen Molecular Weight: Predicted: 97 kDa; apparent: 80 and 125 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged HCP-4 (N-terminal, aa 1-210) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 2E2
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: N-terminal, aa 1-210
Uniprot ID: G5EDA3
Immunogen Sequence: Partial protein
Entrez Gene ID: 171964
Antibody Registry ID: AB_2078913
Additional Information: The CENP-C-like protein HCP-4 is a homolog of CENP-C which localizes to the centromere of chromosomes.
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
HCP4 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product HCP4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References