JB3
Catalog Fields
Antigen: fibrillin 2-like
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Little, C.D.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Kansas Medical Center
Positive Tested Species Reactivity: Chicken, Quail, Xenopus laevis
Depositors Notes: Immunoblotting note: yes, under nonreducing conditions. Immunohistology note: ECM.
Predicted Species Reactivity:
Gene:
Immunogen: membrane-enriched fractions of atrioventricular endocardial cushions
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: unknown
Epitope Location or Sequence:
Antibody Registry ID: AB_531777
Additional Information:
Recommended Applications: Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
JB3 was deposited to the DSHB by Little, C.D. (DSHB Hybridoma Product JB3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
2 References
Initial Publication
Cardiac endothelial heterogeneity defines valvular development as demonstrated by the diverse expression of JB3, an antigen of the endocardial cushion tissue.
Markwald RR
Developmental biology 165.2 (1994 Oct): 585-601.