B3/D6
Catalog Fields
Clone ID/Product Name: B3/D6
Available to For-Profits: Yes
Alternate Antibody Name: B3D6
Gene Symbol: FN1
Ab Isotype: MIgG2a, kappa light chain
Gene Name:
Antibody Registry ID: AB_2105970
Uniprot ID: F1NJT3
Entrez Gene ID: 396133
Clonality: Monoclonal
Immunogen: Purified cellular fibronectin from tertiary chicken embryo fibroblasts
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: SP2/0
Epitope Mapped: No
Antigen Name: fibronectin, avian
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/8/1986
Antigen Molecular Weight: Predicted: 270 kDa; Apparent: 230 kDa (multiple isoforms)
Depositor: Fambrough, D.M.
Antigen Sequence:
Depositor Institution: Johns Hopkins School of Medicine
Antigen Species: chicken
Depositor Notes: Specific for avian fibronectin. Day 11 chicken embryonic myoblasts have extracellular fibronectin fibrils 24 hours after plating. By day 6 of culture 50% of the myotubes have detectable fibronectin, 75% by day 10. There is some intracellular staining of myotubes.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Avian
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B3/D6 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product B3/D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Fibronectin expression during myogenesis.
Fambrough DM
The Journal of cell biology 96.2 (1983 Feb): 474-85.