B3/D6

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$40.00
SKU: B3/D6
View product citations for antibody B3/D6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin, avian
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Johns Hopkins School of Medicine
Positive Tested Species Reactivity: Avian
Depositors Notes: Specific for avian fibronectin. Day 11 chicken embryonic myoblasts have extracellular fibronectin fibrils 24 hours after plating. By day 6 of culture 50% of the myotubes have detectable fibronectin, 75% by day 10. There is some intracellular staining of myotubes.
Antigen Molecular Weight: Predicted: 270 kDa; Apparent: 230 kDa (multiple isoforms)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FN1
Immunogen: Purified cellular fibronectin from tertiary chicken embryo fibroblasts
Alternate Gene Names: FN
Alternate Antibody Name: B3D6
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0
Epitope Location or Sequence:
Uniprot ID: F1NJT3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396133 
Additional Characterization:  
Antibody Registry ID: AB_2105970 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
B3/D6 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product B3/D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

21 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Fibronectin expression during myogenesis.
    Fambrough DM
    The Journal of cell biology 96.2 (1983 Feb): 474-85.

    IF References

    Fibronectin expression during myogenesis.
    Fambrough DM
    The Journal of cell biology 96.2 (1983 Feb): 474-85.

    The accumulation of basement membrane components during the onset of chondrogenesis and myogenesis in the chick wing bud.
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells.
    Solursh M
    Developmental biology 139.2 (1990 Jun): 383-95.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing.
    Chen HM
    The American journal of pathology 138.2 (1991 Feb): 427-40.

    Immunolocalization of basal lamina components during development of chick otic and optic primordia.
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Vascular patterning of the quail coronary system during development.
    Dedkov EI
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 288.9 (2006 Sep): 989-99.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    The endoderm and myocardium join forces to drive early heart tube assembly.
    Rongish BJ
    Developmental biology 404.1 (2015 Aug 1): 40-54.

    Active cell and ECM movements during development.
    Czirók A
    Methods in molecular biology (Clifton, N.J.) 1189. (2015): 123-32.

    Identification of emergent motion compartments in the amniote embryo.
    Rongish BJ
    Organogenesis 10.4 (2014): 350-64.

    Tissue growth constrained by extracellular matrix drives invagination during optic cup morphogenesis.
    Taber LA
    Biomechanics and modeling in mechanobiology 15.6 (2016 Dec): 1405-1421.

    Temporal dissociation of developmental events in the chick eye under low temperature conditions.
    Ishii Y
    Development, growth & differentiation 58.9 (2016 Dec): 741-749.

    WB References

    Fibronectin expression during myogenesis.
    Fambrough DM
    The Journal of cell biology 96.2 (1983 Feb): 474-85.

    Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells.
    Solursh M
    Developmental biology 139.2 (1990 Jun): 383-95.

    IHC References

    Computer-aided mechanogenesis of skeletal muscle organs from single cells in vitro.
    Karlisch P
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 5.13 (1991 Oct): 2860-7.

    An alternative model for cell sheet migration on fibronectin during heart formation.
    Wiens DJ
    Journal of theoretical biology 179.1 (1996 Mar 7): 33-9.

    Simulated microgravity and hypergravity attenuate heart tissue development in explant culture.
    Wiens DJ
    Cells, tissues, organs 167.2-3 (2000): 171-83.

    Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris).
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix.
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    IP References

    Fibronectin expression during myogenesis.
    Fambrough DM
    The Journal of cell biology 96.2 (1983 Feb): 474-85.

    All References

    Computer-aided mechanogenesis of skeletal muscle organs from single cells in vitro.
    Karlisch P
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 5.13 (1991 Oct): 2860-7.

    An alternative model for cell sheet migration on fibronectin during heart formation.
    Wiens DJ
    Journal of theoretical biology 179.1 (1996 Mar 7): 33-9.

    Simulated microgravity and hypergravity attenuate heart tissue development in explant culture.
    Wiens DJ
    Cells, tissues, organs 167.2-3 (2000): 171-83.

    Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris).
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix.
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    Fibronectin expression during myogenesis.
    Fambrough DM
    The Journal of cell biology 96.2 (1983 Feb): 474-85.

    The accumulation of basement membrane components during the onset of chondrogenesis and myogenesis in the chick wing bud.
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    Comparison between ectoderm-conditioned medium and fibronectin in their effects on chondrogenesis by limb bud mesenchymal cells.
    Solursh M
    Developmental biology 139.2 (1990 Jun): 383-95.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Enhanced expression of neural cell adhesion molecules and tenascin (cytotactin) during wound healing.
    Chen HM
    The American journal of pathology 138.2 (1991 Feb): 427-40.

    Immunolocalization of basal lamina components during development of chick otic and optic primordia.
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Vascular patterning of the quail coronary system during development.
    Dedkov EI
    The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 288.9 (2006 Sep): 989-99.

    Expression Patterns of Extracellular Matrix Proteins during Posterior Commissure Development.
    Caprile T
    Frontiers in neuroanatomy 10. (2016): 89.

    The endoderm and myocardium join forces to drive early heart tube assembly.
    Rongish BJ
    Developmental biology 404.1 (2015 Aug 1): 40-54.

    Active cell and ECM movements during development.
    Czirók A
    Methods in molecular biology (Clifton, N.J.) 1189. (2015): 123-32.

    Identification of emergent motion compartments in the amniote embryo.
    Rongish BJ
    Organogenesis 10.4 (2014): 350-64.

    Tissue growth constrained by extracellular matrix drives invagination during optic cup morphogenesis.
    Taber LA
    Biomechanics and modeling in mechanobiology 15.6 (2016 Dec): 1405-1421.

    Temporal dissociation of developmental events in the chick eye under low temperature conditions.
    Ishii Y
    Development, growth & differentiation 58.9 (2016 Dec): 741-749.

    Microinjection of antifibronectin antibodies in the chicken blastoderm: inhibition of mesoblast cell migration but not of cell ingression at the primitive streak.
    Foidart JM
    The Anatomical record 236.4 (1993 Aug): 685-96.

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