M18/2.a.12.7

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$40.00
SKU: M18/2.a.12.7
View product citations for antibody M18/2.a.12.7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Springer, T.A.
Isotype: RIgG2a, kappa light chain
Antigen Sequence:
Host Species: rat
Depositors Institution: Center for Blood Research, Boston
Positive Tested Species Reactivity: Mouse
Depositors Notes: Does not cross react with human antigen. Binds to all leukocytes. Works for western blotting under non-reducing conditions.
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Itgb2
Immunogen: Membrane glycoproteins of cytotoxic T-lymphocytes
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antibody Name: M18/2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Location or Sequence:
Uniprot ID: P11835 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16414 
Additional Characterization:
Antibody Registry ID: AB_2128355 
Additional Information: The effect of M18/2.a.12.7 on function is oftentimes more enhancing than inhibiting. CD18 is a general marker of leukocytes.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M18/2.a.12.7 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M18/2.a.12.7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication

    The Journal of experimental medicine
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    IF References

    The Journal of experimental medicine
    Finlay BB
    The Journal of experimental medicine 186.4 (1997 Aug 18): 569-80.

    Infection and immunity
    García-del Portillo F
    Infection and immunity 81.1 (2013 Jan): 154-65.

    WB References

    The Journal of experimental medicine
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Journal of immunology (Baltimore, Md. : 1950)
    Pierres M
    Journal of immunology (Baltimore, Md. : 1950) 136.10 (1986 May 15): 3750-9.

    IP References

    The Journal of experimental medicine
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Journal of leukocyte biology
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Journal of immunology (Baltimore, Md. : 1950)
    Pierres M
    Journal of immunology (Baltimore, Md. : 1950) 136.10 (1986 May 15): 3750-9.

    FB References

    The Journal of experimental medicine
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Journal of molecular neuroscience : MN
    Rotshenker S
    Journal of molecular neuroscience : MN 21.1 (2003): 65-72.

    Journal of leukocyte biology
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Neurobiology of disease
    Rotshenker S
    Neurobiology of disease 12.1 (2003 Feb): 65-72.

    The Journal of experimental medicine
    Tachman R [corrected to Taichman R]
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1115-27.

    FACS References

    Journal of immunology (Baltimore, Md. : 1950)
    Speert DP
    Journal of immunology (Baltimore, Md. : 1950) 160.11 (1998 Jun 1): 5514-21.

    The Journal of clinical investigation
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    The Journal of experimental medicine
    Tachman R [corrected to Taichman R]
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1115-27.

    Immunology
    Merino J
    Immunology 90.4 (1997 Apr): 550-6.

    All References

    The Journal of experimental medicine
    Finlay BB
    The Journal of experimental medicine 186.4 (1997 Aug 18): 569-80.

    Infection and immunity
    García-del Portillo F
    Infection and immunity 81.1 (2013 Jan): 154-65.

    The Journal of experimental medicine
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Journal of immunology (Baltimore, Md. : 1950)
    Pierres M
    Journal of immunology (Baltimore, Md. : 1950) 136.10 (1986 May 15): 3750-9.

    Journal of leukocyte biology
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Journal of molecular neuroscience : MN
    Rotshenker S
    Journal of molecular neuroscience : MN 21.1 (2003): 65-72.

    Neurobiology of disease
    Rotshenker S
    Neurobiology of disease 12.1 (2003 Feb): 65-72.

    The Journal of experimental medicine
    Tachman R [corrected to Taichman R]
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1115-27.

    Journal of immunology (Baltimore, Md. : 1950)
    Speert DP
    Journal of immunology (Baltimore, Md. : 1950) 160.11 (1998 Jun 1): 5514-21.

    The Journal of clinical investigation
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    Immunology
    Merino J
    Immunology 90.4 (1997 Apr): 550-6.

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