M18/2.a.12.7

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SKU: M18/2.a.12.7
View product citations for antibody M18/2.a.12.7 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD18 (integrin beta-2; beta subunit of CD11a, b, c), murine
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Isotype: RIgG2a, kappa light chain
Depositor: Springer, T.A.
Host Species: rat
Antigen Sequence:
Positive Tested Species Reactivity: Mouse
Depositors Institution: Center for Blood Research, Boston
Antigen Molecular Weight: Predicted: 85 kDa; Apparent: 93 kDa
Depositors Notes: Does not cross react with human antigen. Binds to all leukocytes. Works for western blotting under non-reducing conditions.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Membrane glycoproteins of cytotoxic T-lymphocytes
Gene: Itgb2
Alternate Antibody Name: M18/2
Alternate Gene Names: CD18, MFI7, LAD, LCAMB, LFA-1, MAC-1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.6.5.3
Epitope Mapped: No
Uniprot ID: P11835 
Epitope Location or Sequence:
Entrez Gene ID: 16414 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2128355 
Additional Characterization:  
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: The effect of M18/2.a.12.7 on function is oftentimes more enhancing than inhibiting. CD18 is a general marker of leukocytes.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M18/2.a.12.7 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M18/2.a.12.7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    FB References
    FACS References
    All References

    Murine salmonellosis studied by confocal microscopy: Salmonella typhimurium resides intracellularly inside macrophages and exerts a cytotoxic effect on phagocytes in vivo.
    Finlay BB
    The Journal of experimental medicine 186.4 (1997 Aug 18): 569-80.

    Genome expression analysis of nonproliferating intracellular Salmonella enterica serovar Typhimurium unravels an acid pH-dependent PhoP-PhoQ response essential for dormancy.
    García-del Portillo F
    Infection and immunity 81.1 (2013 Jan): 154-65.

    Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Identification of 5 topographic domains of the mouse LFA-1 molecule: subunit assignment and functional involvement in lymphoid cell interactions.
    Pierres M
    Journal of immunology (Baltimore, Md. : 1950) 136.10 (1986 May 15): 3750-9.

    Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct.
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Microglia and macrophage activation and the regulation of complement-receptor-3 (CR3/MAC-1)-mediated myelin phagocytosis in injury and disease.
    Rotshenker S
    Journal of molecular neuroscience : MN 21.1 (2003): 65-72.

    Complement-receptor-3 and scavenger-receptor-AI/II mediated myelin phagocytosis in microglia and macrophages.
    Rotshenker S
    Neurobiology of disease 12.1 (2003 Feb): 65-72.

    Protein tyrosine kinase p56-Lck regulates lymphocyte function-associated 1 adhesion molecule expression, granule exocytosis, and cytolytic effector function in a cloned T cell.
    Tachman R [corrected to Taichman R]
    The Journal of experimental medicine 180.3 (1994 Sep 1): 1115-27.

    Nonopsonic and opsonic association of Mycobacterium tuberculosis with resident alveolar macrophages is inefficient.
    Speert DP
    Journal of immunology (Baltimore, Md. : 1950) 160.11 (1998 Jun 1): 5514-21.

    An in vivo animal model of gene therapy for leukocyte adhesion deficiency.
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    Treatment with anti-LFA-1 alpha monoclonal antibody selectively interferes with the maturation of CD4- 8+ thymocytes.
    Merino J
    Immunology 90.4 (1997 Apr): 550-6.

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