M1/70.15.11.5.2

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$40.00
SKU: M1/70.15.11.5.2
View product citations for antibody M1/70.15.11.5.2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: CD11b (Integrin alpha-M, Mac-1 alpha chain), murine
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Springer, T.A.
Isotype: RIgG2b
Antigen Sequence:
Host Species: rat
Depositors Institution: Center for Blood Research, Boston
Positive Tested Species Reactivity: Human, Mouse
Depositors Notes: Blocks iC3B rosetting. Binds to granulocytes, monocytes, macrophages, hairy cell leukemia cells, CD5+ human B cells. Doesn't appear to work for western blotting.
Antigen Molecular Weight: Predicted: 128 kDa; Apparent: 170 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Itgam
Immunogen: Spleen cells from B10 mice.
Alternate Gene Names: MAC-1, MO1A, CR3
Alternate Antibody Name: M1/70
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: P05555 
Immunogen Sequence: Full length protein
Entrez Gene ID: 16409 
Additional Characterization:
Antibody Registry ID: AB_2234066 
Additional Information: Mac-1 is a macrophage differenciation antigen and a marker for microglia.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M1/70.15.11.5.2 was deposited to the DSHB by Springer, T.A. (DSHB Hybridoma Product M1/70.15.11.5.2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

24 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • All References
  • Initial Publication
    IF References
    IHC References
    IP References
    FB References

    M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    Best TM
    Journal of applied physiology (Bethesda, Md. : 1985) 95.3 (2003 Sep): 969-76.

    Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Complement-receptor-3 and scavenger-receptor-AI/II mediated myelin phagocytosis in microglia and macrophages.
    Rotshenker S
    Neurobiology of disease 12.1 (2003 Feb): 65-72.

    Integrin regulation of leukocyte inflammatory functions. CD11b/CD18 enhancement of the tumor necrosis factor-alpha responses of monocytes.
    Edgington TS
    Journal of immunology (Baltimore, Md. : 1950) 150.7 (1993 Apr 1): 2972-80.

    Modulation (inhibition and augmentation) of complement receptor-3-mediated myelin phagocytosis.
    Rotshenker S
    Neurobiology of disease 8.3 (2001 Jun): 504-12.

    Comparison of receptors required for entry of Leishmania major amastigotes into macrophages.
    Belosevic M
    Infection and immunity 61.4 (1993 Apr): 1553-8.

    Microglia and macrophage activation and the regulation of complement-receptor-3 (CR3/MAC-1)-mediated myelin phagocytosis in injury and disease.
    Rotshenker S
    Journal of molecular neuroscience : MN 21.1 (2003): 65-72.

    Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
    Rogers C
    Arteriosclerosis, thrombosis, and vascular biology 20.12 (2000 Dec): 2553-8.

    Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.1 (1986 Jul 1): 15-27.

    Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
    Schreiber RD
    The Journal of experimental medicine 156.4 (1982 Oct 1): 1000-9.

    FACS References

    Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
    Milstein C
    European journal of immunology 9.4 (1979 Apr): 301-6.

    Natural killer activity in the peritoneal exudates of mice infected with Listeria monocytogenes: characterization of the natural killer cells by using a monoclonal rat anti-murine macrophage antibody (M1/70).
    Ault KA
    Journal of immunology (Baltimore, Md. : 1950) 127.5 (1981 Nov): 1792-9.

    M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    Best TM
    Journal of applied physiology (Bethesda, Md. : 1985) 95.3 (2003 Sep): 969-76.

    Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 126.1 (1981 Jan): 359-64.

    Novel mouse model to study T cell-dependent IgA induction in vivo.
    Kruglov AA
    Journal of immunological methods 421. (2015 Jun): 54-60.

    IL-10 mediates plasmacytosis-associated immunodeficiency by inhibiting complement-mediated neutrophil migration.
    Manz RA
    The Journal of allergy and clinical immunology 137.5 (2016 May): 1487-1497.e6.

    Static and dynamic components synergize to form a stable survival niche for bone marrow plasma cells.
    Hauser AE
    European journal of immunology 44.8 (2014 Aug): 2306-17.

    The identification of two inhibitory cells in each segmental ganglion of the leech and studies on the ionic mechanism of the inhibitory junctional potentials produced by these cells.
    Coggeshall RE
    Journal of neurobiology 7.5 (1976 Sep): 435-45.

    Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor.
    Springer TA
    The Journal of biological chemistry 257.20 (1982 Oct 25): 12412-8.

    An in vivo animal model of gene therapy for leukocyte adhesion deficiency.
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    All References

    The identification of two inhibitory cells in each segmental ganglion of the leech and studies on the ionic mechanism of the inhibitory junctional potentials produced by these cells.
    Coggeshall RE
    Journal of neurobiology 7.5 (1976 Sep): 435-45.

    Tonic-clonic seizures induce division of neuronal progenitor cells with concomitant changes in expression of neurotrophic factors in the brain of pilocarpine-treated mice.
    Nakano K
    Brain research. Molecular brain research 139.2 (2005 Oct 3): 258-66.

    Fine tuning inflammation at the front door: macrophage complement receptor 3-mediates phagocytosis and immune suppression for Francisella tularensis.
    Schlesinger LS
    PLoS pathogens 9.1 (2013 Jan): e1003114.

    Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
    Milstein C
    European journal of immunology 8.8 (1978 Aug): 539-51.

    Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
    Milstein C
    European journal of immunology 9.4 (1979 Apr): 301-6.

    Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1.
    Springer TA
    The Journal of experimental medicine 158.2 (1983 Aug 1): 586-602.

    Inhibition and stimulation of LFA-1 and Mac-1 functions by antibodies against murine CD18. Evidence that the LFA-1 binding sites for ICAM-1, -2, and -3 are distinct.
    Roos E
    Journal of leukocyte biology 60.6 (1996 Dec): 758-65.

    Purification and structural characterization of LFA-1, a lymphocyte function-associated antigen, and Mac-1, a related macrophage differentiation antigen associated with the type three complement receptor.
    Springer TA
    The Journal of biological chemistry 257.20 (1982 Oct 25): 12412-8.

    M1/70 attenuates blood-borne neutrophil oxidants, activation, and myofiber damage following stretch injury.
    Best TM
    Journal of applied physiology (Bethesda, Md. : 1985) 95.3 (2003 Sep): 969-76.

    Complement-receptor-3 and scavenger-receptor-AI/II mediated myelin phagocytosis in microglia and macrophages.
    Rotshenker S
    Neurobiology of disease 12.1 (2003 Feb): 65-72.

    Integrin regulation of leukocyte inflammatory functions. CD11b/CD18 enhancement of the tumor necrosis factor-alpha responses of monocytes.
    Edgington TS
    Journal of immunology (Baltimore, Md. : 1950) 150.7 (1993 Apr 1): 2972-80.

    Modulation (inhibition and augmentation) of complement receptor-3-mediated myelin phagocytosis.
    Rotshenker S
    Neurobiology of disease 8.3 (2001 Jun): 504-12.

    Comparison of receptors required for entry of Leishmania major amastigotes into macrophages.
    Belosevic M
    Infection and immunity 61.4 (1993 Apr): 1553-8.

    Microglia and macrophage activation and the regulation of complement-receptor-3 (CR3/MAC-1)-mediated myelin phagocytosis in injury and disease.
    Rotshenker S
    Journal of molecular neuroscience : MN 21.1 (2003): 65-72.

    Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
    Rogers C
    Arteriosclerosis, thrombosis, and vascular biology 20.12 (2000 Dec): 2553-8.

    Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 137.1 (1986 Jul 1): 15-27.

    Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
    Schreiber RD
    The Journal of experimental medicine 156.4 (1982 Oct 1): 1000-9.

    Natural killer activity in the peritoneal exudates of mice infected with Listeria monocytogenes: characterization of the natural killer cells by using a monoclonal rat anti-murine macrophage antibody (M1/70).
    Ault KA
    Journal of immunology (Baltimore, Md. : 1950) 127.5 (1981 Nov): 1792-9.

    Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells.
    Springer TA
    Journal of immunology (Baltimore, Md. : 1950) 126.1 (1981 Jan): 359-64.

    Novel mouse model to study T cell-dependent IgA induction in vivo.
    Kruglov AA
    Journal of immunological methods 421. (2015 Jun): 54-60.

    IL-10 mediates plasmacytosis-associated immunodeficiency by inhibiting complement-mediated neutrophil migration.
    Manz RA
    The Journal of allergy and clinical immunology 137.5 (2016 May): 1487-1497.e6.

    Static and dynamic components synergize to form a stable survival niche for bone marrow plasma cells.
    Hauser AE
    European journal of immunology 44.8 (2014 Aug): 2306-17.

    An in vivo animal model of gene therapy for leukocyte adhesion deficiency.
    Wilson JM
    The Journal of clinical investigation 88.4 (1991 Oct): 1412-7.

    Effects of body temperature on neural activity in the hippocampus: regulation of resting membrane potentials by transient receptor potential vanilloid 4.
    Tominaga M
    The Journal of neuroscience : the official journal of the Society for Neuroscience 27.7 (2007 Feb 14): 1566-75.

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