M6

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$40.00
SKU: M6
View product citations for antibody M6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Neuronal membrane glycoprotein M6-a, M6a
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Lagenaur, C.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Mouse
Depositors Notes: The M6a antigen is a membrane glycoprotein expressed on many CNS neurons as well as polarized epithelia in kidney, olfactory epithelium and choroid plexus. M6 also inhibits neurite outgrowth in culture.
Antigen Molecular Weight: 31.1 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Gpm6a
Immunogen: C57BL/6J mouse cerebellar homogenate
Alternate Gene Names: M6a
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Ag8X653
Epitope Location or Sequence:
Uniprot ID: P35802 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 234267 
Additional Characterization:
Antibody Registry ID: AB_2149607 
Additional Information: RRID: AB_2149607
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M6 was deposited to the DSHB by Lagenaur, C. (DSHB Hybridoma Product M6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

16 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    Monoclonal M6 antibody interferes with neurite extension of cultured neurons.
    Schachner M
    Journal of neurobiology 23.1 (1992 Feb): 71-88.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
    Hirata T
    Cerebral cortex (New York, N.Y. : 1991) 25.11 (2015 Nov): 4111-25.

    miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche.
    Doetsch F
    Nature neuroscience 12.4 (2009 Apr): 399-408.

    Axonal growth of embryonic stem cell-derived motoneurons in vitro and in motoneuron-injured adult rats.
    Kerr DA
    Proceedings of the National Academy of Sciences of the United States of America 101.18 (2004 May 4): 7123-8.

    Composition, synthesis, and assembly of the embryonic chick retinal basal lamina.
    Cole GJ
    Developmental biology 220.2 (2000 Apr 15): 111-28.

    Intraretinal grafting reveals growth requirements and guidance cues for optic axons in the developing avian retina.
    Halfter W
    Developmental biology 177.1 (1996 Jul 10): 160-77.

    Grafted murine induced pluripotent stem cells prevent death of injured rat motoneurons otherwise destined to die.
    Dinnyés A
    Experimental neurology 269. (2015 Jul): 188-201.

    Plasticity of cultured mesenchymal stem cells: switch from nestin-positive to excitable neuron-like phenotype.
    Rogister B
    Stem cells (Dayton, Ohio) 23.3 (2005 Mar): 392-402.

    WB References

    Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
    Hirata T
    Cerebral cortex (New York, N.Y. : 1991) 25.11 (2015 Nov): 4111-25.

    IHC References

    Efferent Projections to the Host Brain from Intrastriatal Striatal Mouse-to-rat Grafts: Time Course and Tissue-type Specificity as Revealed by a Mouse Specific Neuronal Marker.
    Björklund A
    The European journal of neuroscience 3.1 (1991 Oct): 86-101.

    Use of a species-specific antibody for demonstrating mouse neurons transplanted to rat brains.
    Lagenaur CF
    Neuroscience letters 61.1-2 (1985 Oct 24): 221-6.

    Expression of members of the proteolipid protein gene family in the developing murine central nervous system.
    Lagenaur C
    The Journal of comparative neurology 370.4 (1996 Jul 8): 465-78.

    Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
    Hirata T
    Cerebral cortex (New York, N.Y. : 1991) 25.11 (2015 Nov): 4111-25.

    Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors.
    Jakobsson J
    Proceedings of the National Academy of Sciences of the United States of America 107.25 (2010 Jun 22): 11602-7.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Expression of members of the proteolipid protein gene family in the developing murine central nervous system.
    Lagenaur C
    The Journal of comparative neurology 370.4 (1996 Jul 8): 465-78.

    Grafted murine induced pluripotent stem cells prevent death of injured rat motoneurons otherwise destined to die.
    Dinnyés A
    Experimental neurology 269. (2015 Jul): 188-201.

    Rescue of injured motoneurones by grafted neuroectodermal stem cells: effect of the location of graft.
    Nógrádi A
    Restorative neurology and neuroscience 31.3 (2013): 263-74.

    Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die.
    Nógrádi A
    Experimental neurology 261. (2014 Nov): 180-9.

    Repair of the injured spinal cord and the potential of embryonic stem cell transplantation.
    Vadivelu S
    Journal of neurotrauma 21.4 (2004 Apr): 383-93.

    IP References

    Monoclonal M6 antibody interferes with neurite extension of cultured neurons.
    Schachner M
    Journal of neurobiology 23.1 (1992 Feb): 71-88.

    FB References

    Monoclonal M6 antibody interferes with neurite extension of cultured neurons.
    Schachner M
    Journal of neurobiology 23.1 (1992 Feb): 71-88.

    Use of a species-specific antibody for demonstrating mouse neurons transplanted to rat brains.
    Lagenaur CF
    Neuroscience letters 61.1-2 (1985 Oct 24): 221-6.

    All References

    Efferent Projections to the Host Brain from Intrastriatal Striatal Mouse-to-rat Grafts: Time Course and Tissue-type Specificity as Revealed by a Mouse Specific Neuronal Marker.
    Björklund A
    The European journal of neuroscience 3.1 (1991 Oct): 86-101.

    Use of a species-specific antibody for demonstrating mouse neurons transplanted to rat brains.
    Lagenaur CF
    Neuroscience letters 61.1-2 (1985 Oct 24): 221-6.

    Expression of members of the proteolipid protein gene family in the developing murine central nervous system.
    Lagenaur C
    The Journal of comparative neurology 370.4 (1996 Jul 8): 465-78.

    Transcallosal Projections Require Glycoprotein M6-Dependent Neurite Growth and Guidance.
    Hirata T
    Cerebral cortex (New York, N.Y. : 1991) 25.11 (2015 Nov): 4111-25.

    Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors.
    Jakobsson J
    Proceedings of the National Academy of Sciences of the United States of America 107.25 (2010 Jun 22): 11602-7.

    Embryonic stem cells develop into functional dopaminergic neurons after transplantation in a Parkinson rat model.
    Isacson O
    Proceedings of the National Academy of Sciences of the United States of America 99.4 (2002 Feb 19): 2344-9.

    Grafted murine induced pluripotent stem cells prevent death of injured rat motoneurons otherwise destined to die.
    Dinnyés A
    Experimental neurology 269. (2015 Jul): 188-201.

    Rescue of injured motoneurones by grafted neuroectodermal stem cells: effect of the location of graft.
    Nógrádi A
    Restorative neurology and neuroscience 31.3 (2013): 263-74.

    Cytokine signaling by grafted neuroectodermal stem cells rescues motoneurons destined to die.
    Nógrádi A
    Experimental neurology 261. (2014 Nov): 180-9.

    Repair of the injured spinal cord and the potential of embryonic stem cell transplantation.
    Vadivelu S
    Journal of neurotrauma 21.4 (2004 Apr): 383-93.

    Monoclonal M6 antibody interferes with neurite extension of cultured neurons.
    Schachner M
    Journal of neurobiology 23.1 (1992 Feb): 71-88.

    miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche.
    Doetsch F
    Nature neuroscience 12.4 (2009 Apr): 399-408.

    Axonal growth of embryonic stem cell-derived motoneurons in vitro and in motoneuron-injured adult rats.
    Kerr DA
    Proceedings of the National Academy of Sciences of the United States of America 101.18 (2004 May 4): 7123-8.

    Composition, synthesis, and assembly of the embryonic chick retinal basal lamina.
    Cole GJ
    Developmental biology 220.2 (2000 Apr 15): 111-28.

    Intraretinal grafting reveals growth requirements and guidance cues for optic axons in the developing avian retina.
    Halfter W
    Developmental biology 177.1 (1996 Jul 10): 160-77.

    Plasticity of cultured mesenchymal stem cells: switch from nestin-positive to excitable neuron-like phenotype.
    Rogister B
    Stem cells (Dayton, Ohio) 23.3 (2005 Mar): 392-402.

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