M6
Catalog Fields
Clone ID/Product Name: M6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: Gpm6a
Ab Isotype: RIgG2a
Gene Name:
Antibody Registry ID: AB_2149607
Uniprot ID: P35802
Entrez Gene ID: 234267
Clonality: Monoclonal
Immunogen: C57BL/6J mouse cerebellar homogenate
Clone:
Immunogen Sequence: Full length sequence
Myeloma Strain: Ag8X653
Epitope Mapped: No
Antigen Name: Neuronal membrane glycoprotein M6-a, M6a
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/11/2001
Antigen Molecular Weight: 31.1 kDa
Depositor: Lagenaur, C.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: Mouse
Depositor Notes: The M6a antigen is a membrane glycoprotein expressed on many CNS neurons as well as polarized epithelia in kidney, olfactory epithelium and choroid plexus. M6 also inhibits neurite outgrowth in culture.
Host Species: rat
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Mouse
Additional Information: RRID: AB_2149607
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M6 was deposited to the DSHB by Lagenaur, C. (DSHB Hybridoma Product M6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Use of a species-specific antibody for demonstrating mouse neurons transplanted to rat brains.
Lagenaur CF
Neuroscience letters 61.1-2 (1985 Oct 24): 221-6.