M2

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$40.00
SKU: M2
View product citations for antibody M2 on CiteAb

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Available: 72

DSHB Data Sheet

Catalog Fields

Antigen: M2; Astrocyte surface antigen (mouse)
Hybridoma Cells Available: Yes
Antigen Species: Mouse
Depositor: Lagenaur, C.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: University of Pittsburgh
Positive Tested Species Reactivity: Mouse
Depositors Notes: The M2 antigen is detected on the cell surface of all GFA protein-positive astrocytes and immature oligodendrocytes. IHC on frozen sections post-fixed with paraformaldehyde or ethanol.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene:
Immunogen: C57BL/6J mouse cerebellar homogenate
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3 NSl/lAg4-1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence: Full length sequence
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531785 
Additional Information: RRID: AB_531785
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
M2 was deposited to the DSHB by Lagenaur, C. (DSHB Hybridoma Product M2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

9 References

  • Initial Publication
  • IF References
  • IHC References
  • All References
  • Initial Publication

    Monoclonal antibody (M2) to glial and neuronal cell surfaces.
    Schachner M
    Journal of supramolecular structure and cellular biochemistry 15.4 (1981): 335-46.

    IF References

    Monoclonal antibody (M2) to glial and neuronal cell surfaces.
    Schachner M
    Journal of supramolecular structure and cellular biochemistry 15.4 (1981): 335-46.

    Timing and patterns of astrocyte migration from xenogeneic transplants of the cortex and corpus callosum.
    Lund RD
    The Journal of comparative neurology 292.2 (1990 Feb 8): 320-30.

    Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells.
    Rogister B
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3295-302.

    Engraftment and differentiation of embryonic stem cell-derived neural progenitor cells in the cochlear nerve trunk: growth of processes into the organ of Corti.
    Edge AS
    Journal of neurobiology 66.13 (2006 Nov): 1489-500.

    miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche.
    Doetsch F
    Nature neuroscience 12.4 (2009 Apr): 399-408.

    Quantitative evaluation of motor function before and after engraftment of dopaminergic neurons in a rat model of Parkinson's disease.
    Liu CS
    Journal of biomedical science 17. (2010 Feb 13): 9.

    Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors.
    Jakobsson J
    Proceedings of the National Academy of Sciences of the United States of America 107.25 (2010 Jun 22): 11602-7.

    Direct conversion of fibroblasts into stably expandable neural stem cells.
    Edenhofer F
    Cell stem cell 10.4 (2012 Apr 6): 473-9.

    Humoral and contact interactions in astroglia/stem cell co-cultures in the course of glia-induced neurogenesis.
    Madarász E
    Glia 49.3 (2005 Feb): 430-44.

    IHC References
    All References

    Timing and patterns of astrocyte migration from xenogeneic transplants of the cortex and corpus callosum.
    Lund RD
    The Journal of comparative neurology 292.2 (1990 Feb 8): 320-30.

    Humoral and contact interactions in astroglia/stem cell co-cultures in the course of glia-induced neurogenesis.
    Madarász E
    Glia 49.3 (2005 Feb): 430-44.

    Monoclonal antibody (M2) to glial and neuronal cell surfaces.
    Schachner M
    Journal of supramolecular structure and cellular biochemistry 15.4 (1981): 335-46.

    Regulation of neural markers nestin and GFAP expression by cultivated bone marrow stromal cells.
    Rogister B
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3295-302.

    Engraftment and differentiation of embryonic stem cell-derived neural progenitor cells in the cochlear nerve trunk: growth of processes into the organ of Corti.
    Edge AS
    Journal of neurobiology 66.13 (2006 Nov): 1489-500.

    miR-124 regulates adult neurogenesis in the subventricular zone stem cell niche.
    Doetsch F
    Nature neuroscience 12.4 (2009 Apr): 399-408.

    Quantitative evaluation of motor function before and after engraftment of dopaminergic neurons in a rat model of Parkinson's disease.
    Liu CS
    Journal of biomedical science 17. (2010 Feb 13): 9.

    Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors.
    Jakobsson J
    Proceedings of the National Academy of Sciences of the United States of America 107.25 (2010 Jun 22): 11602-7.

    Direct conversion of fibroblasts into stably expandable neural stem cells.
    Edenhofer F
    Cell stem cell 10.4 (2012 Apr 6): 473-9.

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