MRN2 2B7

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$40.00
SKU: MRN2 2B7
View product citations for antibody MRN2 2B7 on CiteAb

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Available: 43

DSHB Data Sheet

Catalog Fields

Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG2b, kappa light chain
Depositors Institution: G.V. Montgomery VA Medical Center
Host Species: mouse
Gene: Nr3c2
Positive Tested Species Reactivity: Rat
Alternate Gene Names: Mlr, MCR
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Clonality: Monoclonal
Immunogen: SKEKHELLPYIQQDNSRSG+C (KLH)
Epitope Mapped: Yes
Alternate Antibody Name: rMR 64-82 2B7
Epitope Location or Sequence: N-terminus A/B domain (aa 64-82)
Myeloma Strain: SP2
Immunogen Sequence: Partial protein
Uniprot ID: P22199 
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 64–82 peptide differs between the rat and, human or mouse.
Entrez Gene ID: 25672 
Antibody Registry ID: AB_1157907 
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MRN2 2B7 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product MRN2 2B7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • WB References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication
    WB References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Intact female stroke-prone hypertensive rats lack responsiveness to mineralocorticoid receptor antagonists.
    Dorrance AM
    American journal of physiology. Regulatory, integrative and comparative physiology 293.4 (2007 Oct): R1754-63.

    Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
    Gomez-Sanchez CE
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.7 (2009 Jul): 633-41.

    Diverse immunostaining patterns of mineralocorticoid receptor monoclonal antibodies.
    Gomez-Sanchez EP
    Steroids 76.14 (2011 Dec 20): 1541-5.

    IHC References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
    Gomez-Sanchez CE
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.7 (2009 Jul): 633-41.

    Epitope Map References
    All References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Immunohistochemical demonstration of the mineralocorticoid receptor, 11beta-hydroxysteroid dehydrogenase-1 and -2, and hexose-6-phosphate dehydrogenase in rat ovary.
    Gomez-Sanchez CE
    The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 57.7 (2009 Jul): 633-41.

    Intact female stroke-prone hypertensive rats lack responsiveness to mineralocorticoid receptor antagonists.
    Dorrance AM
    American journal of physiology. Regulatory, integrative and comparative physiology 293.4 (2007 Oct): R1754-63.

    Diverse immunostaining patterns of mineralocorticoid receptor monoclonal antibodies.
    Gomez-Sanchez EP
    Steroids 76.14 (2011 Dec 20): 1541-5.

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