rMR1-18 1D5
Catalog Fields
Product Name/ID: rMR1-18 1D5
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: Nr3c2
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_1157909
Uniprot ID: P22199
Entrez Gene ID: 25672
Clonality: Monoclonal
Immunogen: METKGYHSLPEGLDMERR+C (KLH)
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: SP2
Epitope Mapped: Yes
Antigen Name: mineralocorticoid receptor
Epitope Location or Sequence: N-terminus A/B domain (aa 1-18)
Alternate Antigen Name:
Deposit Date: 10/2/2007
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Depositor: Gomez-Sanchez, C.
Antigen Sequence:
Depositor Institution: G.V. Montgomery VA Medical Center
Antigen Species: Rat
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Canine, Chicken, Human, Mouse, Rabbit, Rat
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 1–18 peptide is identical in the rat, human, and mouse.
Recommended Applications: Chromatin Immunoprecipitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR1-18 1D5 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR1-18 1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
16 References
Initial Publication
Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
Gomez-Sanchez EP
Endocrinology 147.3 (2006 Mar): 1343-8.