rMR1-18 1D5

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$40.00
SKU: rMR1-18 1D5
View product citations for antibody rMR1-18 1D5 on CiteAb

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Available: 53

DSHB Data Sheet

Catalog Fields

Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: G.V. Montgomery VA Medical Center
Positive Tested Species Reactivity: Canine, Chicken, Human, Mouse, Rabbit, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Nr3c2
Immunogen: METKGYHSLPEGLDMERR+C (KLH)
Alternate Gene Names: Mlr, MCR
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 1-18)
Uniprot ID: P22199 
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672 
Additional Characterization:
Antibody Registry ID: AB_1157909 
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 1–18 peptide is identical in the rat, human, and mouse.
Recommended Applications: Chromatin Immunoprecipitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR1-18 1D5 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR1-18 1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

13 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Aldosterone regulates Na(+), K(+) ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.
    Khundmiri SJ
    Biochimica et biophysica acta 1833.10 (2013 Oct): 2143-52.

    Induction of FKBP51 by aldosterone in intestinal epithelium.
    Garty H
    The Journal of steroid biochemistry and molecular biology 139. (2014 Jan): 78-87.

    PKCδ Mediates Mineralocorticoid Receptor Activation by Angiotensin II to Modulate Smooth Muscle Cell Function.
    Jaffe IZ
    Endocrinology 160.9 (2019 Sep 1): 2101-2114.

    WB References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Mineralocorticoid Receptor (MR) trans-Activation of Inflammatory AP-1 Signaling: DEPENDENCE ON DNA SEQUENCE, MR CONFORMATION, AND AP-1 FAMILY MEMBER EXPRESSION.
    Danner RL
    The Journal of biological chemistry 291.45 (2016 Nov 4): 23628-23644.

    Role of Per1 and the mineralocorticoid receptor in the coordinate regulation of αENaC in renal cortical collecting duct cells.
    Gumz ML
    Frontiers in physiology 4. (2013): 253.

    Localization of mineralocorticoid receptors at mammalian synapses.
    Johnson LR
    PloS one 5.12 (2010 Dec 15): e14344.

    Functional characterization of chicken glucocorticoid and mineralocorticoid receptors.
    Porter TE
    American journal of physiology. Regulatory, integrative and comparative physiology 298.5 (2010 May): R1257-68.

    Different expression of membrane-bound and cytosolic hippocampal steroid receptor complexes during spatial training in young male rats.
    Korz V
    European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 24.11 (2014 Nov): 1819-27.

    Aldosterone regulates Na(+), K(+) ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.
    Khundmiri SJ
    Biochimica et biophysica acta 1833.10 (2013 Oct): 2143-52.

    Adrenalectomy amplifies aldosterone induced injury in cardiovascular tissue: an effect attenuated by adrenally derived steroids.
    Gong R
    Steroids 78.3 (2013 Mar): 347-55.

    Induction of FKBP51 by aldosterone in intestinal epithelium.
    Garty H
    The Journal of steroid biochemistry and molecular biology 139. (2014 Jan): 78-87.

    PKCδ Mediates Mineralocorticoid Receptor Activation by Angiotensin II to Modulate Smooth Muscle Cell Function.
    Jaffe IZ
    Endocrinology 160.9 (2019 Sep 1): 2101-2114.

    IHC References
    IP References
    ELISA References
    Epitope Map References
    All References

    Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
    Gomez-Sanchez EP
    Endocrinology 147.3 (2006 Mar): 1343-8.

    Localization of mineralocorticoid receptors at mammalian synapses.
    Johnson LR
    PloS one 5.12 (2010 Dec 15): e14344.

    Aldosterone regulates Na(+), K(+) ATPase activity in human renal proximal tubule cells through mineralocorticoid receptor.
    Khundmiri SJ
    Biochimica et biophysica acta 1833.10 (2013 Oct): 2143-52.

    Adult-onset hypothyroidism enhances fear memory and upregulates mineralocorticoid and glucocorticoid receptors in the amygdala.
    Guadaño-Ferraz A
    PloS one 6.10 (2011): e26582.

    Induction of FKBP51 by aldosterone in intestinal epithelium.
    Garty H
    The Journal of steroid biochemistry and molecular biology 139. (2014 Jan): 78-87.

    PKCδ Mediates Mineralocorticoid Receptor Activation by Angiotensin II to Modulate Smooth Muscle Cell Function.
    Jaffe IZ
    Endocrinology 160.9 (2019 Sep 1): 2101-2114.

    Mineralocorticoid Receptor (MR) trans-Activation of Inflammatory AP-1 Signaling: DEPENDENCE ON DNA SEQUENCE, MR CONFORMATION, AND AP-1 FAMILY MEMBER EXPRESSION.
    Danner RL
    The Journal of biological chemistry 291.45 (2016 Nov 4): 23628-23644.

    Role of Per1 and the mineralocorticoid receptor in the coordinate regulation of αENaC in renal cortical collecting duct cells.
    Gumz ML
    Frontiers in physiology 4. (2013): 253.

    Functional characterization of chicken glucocorticoid and mineralocorticoid receptors.
    Porter TE
    American journal of physiology. Regulatory, integrative and comparative physiology 298.5 (2010 May): R1257-68.

    Different expression of membrane-bound and cytosolic hippocampal steroid receptor complexes during spatial training in young male rats.
    Korz V
    European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology 24.11 (2014 Nov): 1819-27.

    Adrenalectomy amplifies aldosterone induced injury in cardiovascular tissue: an effect attenuated by adrenally derived steroids.
    Gong R
    Steroids 78.3 (2013 Mar): 347-55.

    Modulation of transcriptional mineralocorticoid receptor activity by nitrosative stress.
    Grossmann C
    Free radical biology & medicine 53.5 (2012 Sep 1): 1088-100.

    Mineralocorticoid receptor interaction with SP1 generates a new response element for pathophysiologically relevant gene expression.
    Grossmann C
    Nucleic acids research 41.17 (2013 Sep): 8045-60.

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