rMR1-18 1D5
Catalog Fields
Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: G.V. Montgomery VA Medical Center
Positive Tested Species Reactivity: Canine, Chicken, Human, Mouse, Rabbit, Rat
Depositors Notes:
Predicted Species Reactivity:
Gene: Nr3c2
Immunogen: METKGYHSLPEGLDMERR+C (KLH)
Alternate Gene Names: Mlr, MCR
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 1-18)
Uniprot ID: P22199
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672
Antibody Registry ID: AB_1157909
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 1–18 peptide is identical in the rat, human, and mouse.
Recommended Applications: Chromatin Immunoprecipitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR1-18 1D5 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR1-18 1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
Gomez-Sanchez EP
Endocrinology 147.3 (2006 Mar): 1343-8.