rMR1-18 1D5
Catalog Fields
Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: G.V. Montgomery VA Medical Center
Positive Tested Species Reactivity: Canine, Chicken, Human, Mouse, Rabbit, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: Nr3c2
Immunogen: METKGYHSLPEGLDMERR+C (KLH)
Alternate Gene Names: Mlr, MCR
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 1-18)
Uniprot ID: P22199
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672
Additional Characterization:
Antibody Registry ID: AB_1157909
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 1–18 peptide is identical in the rat, human, and mouse.
Recommended Applications: Chromatin Immunoprecipitation, ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR1-18 1D5 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR1-18 1D5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Development of a panel of monoclonal antibodies against the mineralocorticoid receptor.
Gomez-Sanchez EP
Endocrinology 147.3 (2006 Mar): 1343-8.