MT1
Catalog Fields
Clone ID/Product Name: MT1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: TNC
Ab Isotype: MIgM
Gene Name:
Clonality: Monoclonal
Immunogen: Blastema crude lysate
Clone:
Immunogen Sequence: Full length
Myeloma Strain: NS-1
Epitope Mapped: No
Antigen Name: Tenascin-C
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 6/12/2001
Antigen Molecular Weight:
Depositor: Tassava, R.A.
Antigen Sequence:
Depositor Institution: Ohio State University
Antigen Species: Notophthalmus viridescens (newt)
Depositor Notes: Western blot under non-reducing conditions ONLY.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Axolotl, Newt
Additional Information: The MT1 mAb was previously called 4G3.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MT1 was deposited to the DSHB by Tassava, R.A. (DSHB Hybridoma Product MT1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
4 References
Initial Publication