Mab 3F52

(0) No Reviews yet
$40.00
SKU: Mab 3F52-s
View product citations for antibody Mab 3F52 on CiteAb

In Stock

Available: 179

DSHB Data Sheet

Catalog Fields

Antigen: Glucagon-like peptide 1 receptor
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Knudsen, L.B.
Isotype: MIgG1
Depositors Institution: Novo Nordisk A/S
Host Species: mouse
Depositors Notes: GLP1R knockout mice on a C57BL background were used for immunization. The antibody is predicted to be nonreactive in species lacking Trp33. These species include mouse, rat, goat, rabbit, pig, cow, cat and dog.
Positive Tested Species Reactivity: Human, Primate
Gene: GLP1R
Antigen Molecular Weight: 53.0 kDa
Clonality: Monoclonal
Immunogen: GLP-1R extracellular domain (refolded)
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: 24467746
Uniprot ID: P43220 
Immunogen Sequence: aa 24-145
Entrez Gene ID: 2740 
Additional Information: Purification of the E. coli recombinant extracellular domain of GLP-1 has been described (PUBMED 17444618).
Antibody Registry ID: AB_2618100 
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 3F52 was deposited to the DSHB by Knudsen, L.B. (DSHB Hybridoma Product Mab 3F52)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    ELISA References
    FFPE References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    FB References
    Epitope Map References
    All References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    The GLP1R Agonist Liraglutide Reduces Hyperglucagonemia Induced by the SGLT2 Inhibitor Dapagliflozin via Somatostatin Release.
    Bonner C
    Cell reports 28.6 (2019 Aug 6): 1447-1454.e4.

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review