Mab 3F52

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$40.00
SKU: Mab 3F52-s
View product citations for antibody Mab 3F52 on CiteAb

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Available: 173

DSHB Data Sheet

Catalog Fields

Antigen: Glucagon-like peptide 1 receptor
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Knudsen, L.B.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Novo Nordisk A/S
Positive Tested Species Reactivity: Human, Primate
Depositors Notes: GLP1R knockout mice on a C57BL background were used for immunization. The antibody is predicted to be nonreactive in species lacking Trp33. These species include mouse, rat, goat, rabbit, pig, cow, cat and dog.
Antigen Molecular Weight: 53.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GLP1R
Immunogen: GLP-1R extracellular domain (refolded)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: 24467746
Uniprot ID: P43220 
Immunogen Sequence: aa 24-145
Entrez Gene ID: 2740 
Additional Characterization:  
Antibody Registry ID: AB_2618100 
Additional Information: Purification of the E. coli recombinant extracellular domain of GLP-1 has been described (PUBMED 17444618).
Recommended Applications: ELISA, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 3F52 was deposited to the DSHB by Knudsen, L.B. (DSHB Hybridoma Product Mab 3F52)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • ELISA References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References
    IHC References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    ELISA References
    FFPE References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    FB References
    Epitope Map References
    All References

    GLP-1 receptor localization in monkey and human tissue: novel distribution revealed with extensively validated monoclonal antibody.
    Knudsen LB
    Endocrinology 155.4 (2014 Apr): 1280-90.

    Glucagon-like-peptide-1 receptor expression in normal and diseased human thyroid and pancreas.
    Reubi JC
    Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 28.3 (2015 Mar): 391-402.

    The GLP1R Agonist Liraglutide Reduces Hyperglucagonemia Induced by the SGLT2 Inhibitor Dapagliflozin via Somatostatin Release.
    Bonner C
    Cell reports 28.6 (2019 Aug 6): 1447-1454.e4.

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