Mab 7F38

(0)No Reviews yet
$50.00
QuantityPrice
1 – 19$50.00
20 – 49$35.00
50 – 99$32.00
100 +$30.00
SKU: Mab 7F38-s

In Stock

Available: 179

DSHB Data Sheet

Catalog Fields

Product Name/ID: Mab 7F38
Available to For-Profits: Yes
Alternate Antibody Name: 7F38A2
Gene Name: GLP1R
Ab Isotype: MIgG2a, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2618101 
Uniprot ID: P43220 
RRID:  
Entrez Gene ID: 2740 
Clonality: Monoclonal
Immunogen: BHK cells overexpressing mouse Glucagon-like peptide 1 receptor
Clone:
Immunogen Sequence: full-length
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: No
Antigen Name: Glucagon-like peptide 1 receptor
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 2/25/2016
Antigen Molecular Weight: 53.0 kDa
Depositor: Knudsen, L.B.
Antigen Sequence:
Depositor Institution: Novo Nordisk A/S
Antigen Species: Mouse
Depositor Notes: GLP1R knockout mice on a C57BL background were used for immunization.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Mouse, Rabbit, Rat
Additional Information: Purification of the E. coli recombinant extracellular domain of GLP-1 has been described (PUBMED 17444618). RRID:AB_2618101
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 7F38 was deposited to the DSHB by Knudsen, L.B. (DSHB Hybridoma Product Mab 7F38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

    WB References

    Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro.
    Sango K
    International journal of molecular sciences 22.6 (2021 Mar 15): .

    IHC References
    FFPE References
    All References

    Activation of GLP-1 receptors on vascular smooth muscle cells reduces the autoregulatory response in afferent arterioles and increases renal blood flow.
    Sorensen CM
    American journal of physiology. Renal physiology 308.8 (2015 Apr 15): F867-77.

    Evaluation of glucagon-like peptide-1 receptor expression in nondiabetic and diabetic atherosclerotic mice using PET tracer (68)Ga-NODAGA-exendin-4.
    Roivainen A
    American journal of physiology. Endocrinology and metabolism 320.5 (2021 May 1): E989-E998.

    PDX1(LOW) MAFA(LOW) β-cells contribute to islet function and insulin release.
    Hodson DJ
    Nature communications 12.1 (2021 Jan 29): 674.

    Exendin-4 Promotes Schwann Cell Survival/Migration and Myelination In Vitro.
    Sango K
    International journal of molecular sciences 22.6 (2021 Mar 15): .

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review