Mab 7F38

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SKU: Mab 7F38-s
View product citations for antibody Mab 7F38 on CiteAb

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Available: 105

DSHB Data Sheet

Catalog Fields

Antigen: Glucagon-like peptide 1 receptor
Hybridoma Cells Available: No
Antigen Species: Mouse
Depositor: Knudsen, L.B.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Novo Nordisk A/S
Positive Tested Species Reactivity: Human, Mouse, Rabbit, Rat
Depositors Notes: GLP1R knockout mice on a C57BL background were used for immunization.
Antigen Molecular Weight: 53.0 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: GLP1R
Immunogen: BHK cells overexpressing mouse Glucagon-like peptide 1 receptor
Alternate Gene Names:
Alternate Antibody Name: 7F38A2
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence:
Uniprot ID: P43220 
Immunogen Sequence: full-length
Entrez Gene ID: 2740 
Additional Characterization:  
Antibody Registry ID: AB_2618101 
Additional Information: Purification of the E. coli recombinant extracellular domain of GLP-1 has been described (PUBMED 17444618). RRID:AB_2618101
Recommended Applications: ELISA, FACS, FFPE, Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
Mab 7F38 was deposited to the DSHB by Knudsen, L.B. (DSHB Hybridoma Product Mab 7F38)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • IF References
  • IHC References
  • FFPE References
  • All References
  • Initial Publication
    IF References
    IHC References
    FFPE References
    All References

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