P1E6
Catalog Fields
Antigen: integrin alpha 2 (CD49b)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Wayner, E.A. / Carter, W.G.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 150 kDa
Depositors Notes: Inhibits binding to collagens I, III, IV and VI. Will IP alpha2 beta1 complex (non-reducing). Immunoblotting works under non-reducing conditions. Immunostaining with frozen or acetone fixation (not formalin). Doesn't work for FFPE.
Immunogen: Human HT-1080 fibrosarcoma cells
Gene: ITGA2
Alternate Antibody Name: CD49b
Alternate Gene Names: BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Uniprot ID: P17301
Epitope Location or Sequence: I domain (aa 169-378 of Uniprot ID P17301-1)
Entrez Gene ID: 3673
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2619597
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: P1E6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1E6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
15 References
Initial Publication
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Carter WG
The Journal of cell biology 105.4 (1987 Oct): 1873-84.