P1E6
Catalog Fields
Antigen: integrin alpha 2 (CD49b)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits binding to collagens I, III, IV and VI. Will IP alpha2 beta1 complex (non-reducing). Immunoblotting works under non-reducing conditions. Immunostaining with frozen or acetone fixation (not formalin). Doesn't work for FFPE.
Predicted Species Reactivity:
Gene: ITGA2
Immunogen: Human HT-1080 fibrosarcoma cells
Alternate Gene Names: BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2
Alternate Antibody Name: CD49b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: I domain (aa 169-378 of Uniprot ID P17301-1)
Uniprot ID: P17301
Immunogen Sequence: Full length protein
Entrez Gene ID: 3673
Antibody Registry ID: AB_2619597
Additional Information: P1E6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1E6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
15 References
Initial Publication
Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
Carter WG
The Journal of cell biology 105.4 (1987 Oct): 1873-84.