P1E6

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$40.00
SKU: P1E6
View product citations for antibody P1E6 on CiteAb

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Available: 23

DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha 2 (CD49b)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Carter, W.G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Fred Hutchison Cancer Research Center,
Positive Tested Species Reactivity: Human
Depositors Notes: Inhibits binding to collagens I, III, IV and VI. Will IP alpha2 beta1 complex (non-reducing). Immunoblotting works under non-reducing conditions. Immunostaining with frozen or acetone fixation (not formalin). Doesn't work for FFPE.
Antigen Molecular Weight: Predicted: 129 kDa; Apparent: 150 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ITGA2
Immunogen: Human HT-1080 fibrosarcoma cells
Alternate Gene Names: BR, GPIa, CD49B, HPA-5, VLA-2, VLAA2
Alternate Antibody Name: CD49b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-1/FOX-NY
Epitope Location or Sequence: I domain (aa 169-378 of Uniprot ID P17301-1)
Uniprot ID: P17301 
Immunogen Sequence: Full length protein
Entrez Gene ID: 3673 
Additional Characterization:  
Antibody Registry ID: AB_2619597 
Additional Information: P1E6 recognizes an epitope that requires activation of the alpha2/beta1 complex.
Recommended Applications: FACS, Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P1E6 was deposited to the DSHB by Wayner, E.A. / Carter, W.G. (DSHB Hybridoma Product P1E6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Differential expression of integrins and laminin-5 in normal oral epithelia.
    Reibel J
    APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 105.7 (1997 Jul): 519-30.

    IHC References
    IP References
    FB References
    FACS References
    Epitope Map References

    Identification of putative ligand binding sites within I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29)
    Takada Y
    The Journal of biological chemistry 269.13 (1994 Apr 1): 9659-63.

    Integrin alpha 2 I-domain is a binding site for collagens.
    Humphries MJ
    Journal of cell science 108 ( Pt 4). (1995 Apr): 1629-37.

    All References

    The role of integrins alpha 2 beta 1 and alpha 3 beta 1 in cell-cell and cell-substrate adhesion of human epidermal cells.
    Kaur P
    The Journal of cell biology 110.4 (1990 Apr): 1387-404.

    Integrin expression and differentiation in transformed human epidermal cells is regulated by fibroblasts.
    Carter WG
    Journal of cell science 103 ( Pt 3). (1992 Nov): 755-63.

    Differential expression of integrins and laminin-5 in normal oral epithelia.
    Reibel J
    APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 105.7 (1997 Jul): 519-30.

    Identification of multiple cell adhesion receptors for collagen and fibronectin in human fibrosarcoma cells possessing unique alpha and common beta subunits.
    Carter WG
    The Journal of cell biology 105.4 (1987 Oct): 1873-84.

    Fibrillar collagen inhibits arterial smooth muscle proliferation through regulation of Cdk2 inhibitors.
    Ross R
    Cell 87.6 (1996 Dec 13): 1069-78.

    C-propeptides of procollagens I alpha 1 and II that differentially accumulate in enchondromas versus chondrosarcomas regulate tumor cell survival and migration.
    Magdalou J
    Cancer research 70.11 (2010 Jun 1): 4739-48.

    Cooperation between IL-7 Receptor and Integrin α2β1 (CD49b) Drives Th17-Mediated Bone Loss.
    Aoudjit F
    Journal of immunology (Baltimore, Md. : 1950) 195.9 (2015 Nov 1): 4198-209.

    Elucidation of the Roles of Tumor Integrin β1 in the Extravasation Stage of the Metastasis Cascade.
    Kamm RD
    Cancer research 76.9 (2016 May 1): 2513-24.

    Deficiency of the integrin beta 4 subunit in junctional epidermolysis bullosa with pyloric atresia: consequences for hemidesmosome formation and adhesion properties.
    Sonnenberg A
    Journal of cell science 109 ( Pt 7). (1996 Jul): 1695-706.

    Gelatin scaffold combined with bone morphogenetic protein-4 induces odontoblast-like cell differentiation involving integrin profile changes, autophagy-related gene 10, and Wnt5 sequentially in human induced pluripotent stem cells.
    Mogi M
    Differentiation; research in biological diversity 93. (2017 Jan - Feb): 1-14.

    Enhanced Expression of Integrin αvβ3 Induced by TGF-β Is Required for the Enhancing Effect of Fibroblast Growth Factor 1 (FGF1) in TGF-β-Induced Epithelial-Mesenchymal Transition (EMT) in Mammary Epithelial Cells.
    Matsuura N
    PloS one 10.9 (2015): e0137486.

    Differentiation of human skeletal muscle stem cells into odontoblasts is dependent on induction of α1 integrin expression.
    Kramer RH
    The Journal of biological chemistry 289.20 (2014 May 16): 14380-91.

    Identification of putative ligand binding sites within I domain of integrin alpha 2 beta 1 (VLA-2, CD49b/CD29)
    Takada Y
    The Journal of biological chemistry 269.13 (1994 Apr 1): 9659-63.

    Integrin alpha 2 I-domain is a binding site for collagens.
    Humphries MJ
    Journal of cell science 108 ( Pt 4). (1995 Apr): 1629-37.

    Clustering of syndecan-4 and integrin beta1 by laminin alpha 3 chain-derived peptide promotes keratinocyte migration.
    Utani A
    Molecular biology of the cell 20.13 (2009 Jul): 3012-24.

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