P3D1

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$45.00 to $425.00
SKU: P3D1
View product citations for antibody P3D1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: endoglin
Available to For-Profits: Yes
Antigen Species: human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG2a
Depositor: Wayner, E.A. / Vercellotti, G.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: 90 kDa (reduced)
Depositors Notes:
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: IL-1beta activated human umbilical vein endothelial cells
Gene:
Alternate Antibody Name: CD105
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Fox NY
Epitope Mapped: No
Uniprot ID:  
Epitope Location or Sequence:
Entrez Gene ID:  
Immunogen Sequence:
Antibody Registry ID: AB_528221 
Additional Characterization:  
Recommended Applications: FACS, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P3D1 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P3D1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

1 Reference

  • WB References
  • FACS References
  • All References
  • WB References

    Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Bernabeu C
    Cellular and molecular life sciences : CMLS 75.7 (2018 Apr): 1269-1284.

    FACS References

    Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Bernabeu C
    Cellular and molecular life sciences : CMLS 75.7 (2018 Apr): 1269-1284.

    All References

    Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Bernabeu C
    Cellular and molecular life sciences : CMLS 75.7 (2018 Apr): 1269-1284.

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