P4A4

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$40.00
SKU: P4A4
View product citations for antibody P4A4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Endoglin/CD105/TGF-receptor III complex
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Wayner, E.A. / Vercellotti, G.
Isotype: MIgG2b, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Minnesota
Positive Tested Species Reactivity: Human, Monkey, Mouse, Porcine, Rat
Depositors Notes: P4A4 may also be used to activate T cell binding to endothelium.
Antigen Molecular Weight: 90 kDa (monomer) and 180 kDa (dimer)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: ENG
Immunogen: Human unbilical vein endothelial cells
Alternate Gene Names: CD105, END, HHT1, ORW, ORW1
Alternate Antibody Name: CD105
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS-I/FOX-NY
Epitope Location or Sequence: a.a. 277-331
Uniprot ID: P17813 
Immunogen Sequence: Full length sequence
Entrez Gene ID: 2022 
Additional Characterization:  
Antibody Registry ID: AB_528222 
Additional Information: RRID: AB_528222
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P4A4 was deposited to the DSHB by Wayner, E.A. / Vercellotti, G. (DSHB Hybridoma Product P4A4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    WB References

    Endoglin controls cell migration and composition of focal adhesions: function of the cytosolic domain.
    Vary CP
    The Journal of biological chemistry 279.26 (2004 Jun 25): 27440-9.

    5'UTR mutations of ENG cause hereditary hemorrhagic telangiectasia.
    Bayrak-Toydemir P
    Orphanet journal of rare diseases 6. (2011 Dec 22): 85.

    Mapping epitopes to distinct regions of the extracellular domain of endoglin using bacterially expressed recombinant fragments.
    Letarte M
    Tissue antigens 50.3 (1997 Sep): 265-76.

    Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling.
    Bernabeu C
    Journal of cellular physiology 210.2 (2007 Feb): 456-68.

    5'UTR mutations of ENG cause hereditary hemorrhagic telangiectasia.
    Bayrak-Toydemir P
    Orphanet journal of rare diseases 6. (2011 Dec 22): 85.

    CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells.
    Hauck CR
    The Journal of cell biology 170.5 (2005 Aug 29): 825-36.

    IHC References

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    IP References
    FB References
    FACS References

    Immunosuppressor FK506 increases endoglin and activin receptor-like kinase 1 expression and modulates transforming growth factor-β1 signaling in endothelial cells.
    Botella LM
    Molecular pharmacology 79.5 (2011 May): 833-43.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Expression of endoglin isoforms in the myeloid lineage and their role during aging and macrophage polarization.
    Bernabeu C
    Journal of cell science 127.Pt 12 (2014 Jun 15): 2723-35.

    Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.
    Hauck CR
    PLoS pathogens 12.5 (2016 May): e1005608.

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    Propranolol as antiangiogenic candidate for the therapy of hereditary haemorrhagic telangiectasia.
    Botella LM
    Thrombosis and haemostasis 108.1 (2012 Jul): 41-53.

    Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling.
    Bernabeu C
    Journal of cellular physiology 210.2 (2007 Feb): 456-68.

    Effects of oxygen and culture system on in vitro propagation and redifferentiation of osteoarthritic human articular chondrocytes.
    Leavesley DI
    Cell and tissue research 347.3 (2012 Mar): 649-63.

    CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells.
    Hauck CR
    The Journal of cell biology 170.5 (2005 Aug 29): 825-36.

    Bazedoxifene, a new orphan drug for the treatment of bleeding in hereditary haemorrhagic telangiectasia.
    Botella LM
    Thrombosis and haemostasis 115.6 (2016 Jun 2): 1167-77.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Bernabeu C
    Cellular and molecular life sciences : CMLS 75.7 (2018 Apr): 1269-1284.

    Epitope Map References
    All References

    Surface protein characterization of human adipose tissue-derived stromal cells.
    Gimble JM
    Journal of cellular physiology 189.1 (2001 Oct): 54-63.

    Endoglin controls cell migration and composition of focal adhesions: function of the cytosolic domain.
    Vary CP
    The Journal of biological chemistry 279.26 (2004 Jun 25): 27440-9.

    Uropathogenic E. coli Exploit CEA to Promote Colonization of the Urogenital Tract Mucosa.
    Hauck CR
    PLoS pathogens 12.5 (2016 May): e1005608.

    5'UTR mutations of ENG cause hereditary hemorrhagic telangiectasia.
    Bayrak-Toydemir P
    Orphanet journal of rare diseases 6. (2011 Dec 22): 85.

    Mapping epitopes to distinct regions of the extracellular domain of endoglin using bacterially expressed recombinant fragments.
    Letarte M
    Tissue antigens 50.3 (1997 Sep): 265-76.

    Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling.
    Bernabeu C
    Journal of cellular physiology 210.2 (2007 Feb): 456-68.

    CEACAM engagement by human pathogens enhances cell adhesion and counteracts bacteria-induced detachment of epithelial cells.
    Hauck CR
    The Journal of cell biology 170.5 (2005 Aug 29): 825-36.

    Endoglin structure and function: Determinants of endoglin phosphorylation by transforming growth factor-beta receptors.
    Vary CP
    The Journal of biological chemistry 281.35 (2006 Sep 1): 25110-23.

    Immunosuppressor FK506 increases endoglin and activin receptor-like kinase 1 expression and modulates transforming growth factor-β1 signaling in endothelial cells.
    Botella LM
    Molecular pharmacology 79.5 (2011 May): 833-43.

    Stage-specific embryonic antigen-4 is not a marker for chondrogenic and osteogenic potential in cultured chondrocytes and mesenchymal progenitor cells.
    Klein TJ
    Tissue engineering. Part A 19.11-12 (2013 Jun): 1316-26.

    Expression of endoglin isoforms in the myeloid lineage and their role during aging and macrophage polarization.
    Bernabeu C
    Journal of cell science 127.Pt 12 (2014 Jun 15): 2723-35.

    Propranolol as antiangiogenic candidate for the therapy of hereditary haemorrhagic telangiectasia.
    Botella LM
    Thrombosis and haemostasis 108.1 (2012 Jul): 41-53.

    Effects of oxygen and culture system on in vitro propagation and redifferentiation of osteoarthritic human articular chondrocytes.
    Leavesley DI
    Cell and tissue research 347.3 (2012 Mar): 649-63.

    Bazedoxifene, a new orphan drug for the treatment of bleeding in hereditary haemorrhagic telangiectasia.
    Botella LM
    Thrombosis and haemostasis 115.6 (2016 Jun 2): 1167-77.

    Human endoglin as a potential new partner involved in platelet-endothelium interactions.
    Bernabeu C
    Cellular and molecular life sciences : CMLS 75.7 (2018 Apr): 1269-1284.

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