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View product citations for antibody P3G8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: integrin alpha V subunit (CD51, fibronectin receptor alpha subunit)
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Wayner, E.A.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human
Depositors Institution: Fred Hutchinson Cancer Research Center
Antigen Molecular Weight: Predicted: 111 to 116 kDa
Depositors Notes: Immunostaining: Fixation, frozen (acetone), paraformaldehyde; Paraffin, yes – formalin fixed; Special protocols, Antigen Retrieval with 10mM sodium citrate pH 6.0. Doesn't appear to work for Immunoblotting.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Small lung carcinoma cells (UCLA P3).
Alternate Antibody Name: CD51
Alternate Gene Names: MSK8, VNRA, CD51, VTNR
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Uniprot ID: P06756 
Epitope Location or Sequence: The epitope is located in the extracellular region; aa 528 of human alpha-v integrin (aa 558 of Uniprot P06756-1) is required for P3G8 binding.
Entrez Gene ID: 3685 
Immunogen Sequence: Full length protein
Antibody Registry ID: AB_2619589 
Additional Characterization:  
Recommended Applications: ELISA, FACS, FFPE, Immunohistochemistry, Immunoprecipitation
Additional Information: P3G8 recognizes all isoforms.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P3G8 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P3G8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

11 References

  • Initial Publication
  • IHC References
  • IP References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IHC References

    Prognostic value of beta1 integrin expression in metastatic melanoma.
    Pyrhönen S
    Melanoma research 10.3 (2000 Jun): 243-51.

    IP References
    FACS References
    Epitope Map References
    All References

    Prognostic value of beta1 integrin expression in metastatic melanoma.
    Pyrhönen S
    Melanoma research 10.3 (2000 Jun): 243-51.

    Role of the integrin alpha v beta 6 in cell attachment to fibronectin. Heterologous expression of intact and secreted forms of the receptor.
    Sheppard D
    The Journal of biological chemistry 269.9 (1994 Mar 4): 6940-8.

    The decreased adhesion of Y79 retinoblastoma cells to extracellular matrix proteins is due to a deficit of integrin receptors.
    Cameron JD
    Investigative ophthalmology & visual science 35.6 (1994 May): 2820-33.

    Functional differences between integrin alpha4 and integrins alpha5/alphav in modulating the motility of human oral squamous carcinoma cells in response to the V region and heparin-binding domain of fibronectin.
    Kapila Y
    Experimental cell research 295.1 (2004 Apr 15): 48-58.

    Cytokines increase human hemopoietic cell adhesiveness by activation of very late antigen (VLA)-4 and VLA-5 integrins.
    Simmons PJ
    The Journal of experimental medicine 181.5 (1995 May 1): 1805-15.

    Epitope mapping for monoclonal antibody reveals the activation mechanism for αVβ3 integrin.
    Aiso S
    PloS one 8.6 (2013): e66096.

    Identification of motifs in the fasciclin domains of the transforming growth factor-beta-induced matrix protein betaig-h3 that interact with the alphavbeta5 integrin.
    Kim IS
    The Journal of biological chemistry 277.48 (2002 Nov 29): 46159-65.

    Involvement of integrins with adhesion-promoting, heparin-binding peptides of type IV collagen in cultured human corneal epithelial cells.
    Furcht LT
    Investigative ophthalmology & visual science 36.2 (1995 Feb): 364-72.

    Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
    Cheresh DA
    The Journal of cell biology 113.4 (1991 May): 919-29.

    Adhesion of endothelial cells to NOV is mediated by the integrins alphavbeta3 and alpha5beta1.
    Kemp PR
    Journal of vascular research 40.3 (2003 May-Jun): 234-43.

    Alpha2beta1 integrin regulates lineage commitment in multipotent human colorectal cancer cells.
    Ying H
    The Journal of biological chemistry 283.41 (2008 Oct 10): 27612-27619.

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