P3G8
Catalog Fields
Clone ID/Product Name: P3G8
Available to For-Profits: Yes
Alternate Antibody Name: CD51
Gene Symbol: ITGAV
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_2619589
Uniprot ID: P06756
Entrez Gene ID: 3685
Clonality: Monoclonal
Immunogen: Small lung carcinoma cells (UCLA P3).
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: NS-1/FOX-NY
Epitope Mapped: Yes
Antigen Name: integrin alpha V subunit (CD51, fibronectin receptor alpha subunit)
Epitope Location or Sequence: The epitope is located in the extracellular region; aa 528 of human alpha-v integrin (aa 558 of Uniprot P06756-1) is required for P3G8 binding.
Alternate Antigen Name:
Deposit Date: 10/11/2011
Antigen Molecular Weight: Predicted: 111 to 116 kDa
Depositor: Wayner, E.A.
Antigen Sequence:
Depositor Institution: Fred Hutchinson Cancer Research Center
Antigen Species: Human
Depositor Notes: Immunostaining: Fixation, frozen (acetone), paraformaldehyde; Paraffin, yes – formalin fixed; Special protocols, Antigen Retrieval with 10mM sodium citrate pH 6.0. Doesn't appear to work for Immunoblotting.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human
Additional Information: P3G8 recognizes all isoforms.
Recommended Applications: ELISA, FACS, FFPE, Immunohistochemistry, Immunoprecipitation
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
P3G8 was deposited to the DSHB by Wayner, E.A. (DSHB Hybridoma Product P3G8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
11 References
Initial Publication
Integrins alpha v beta 3 and alpha v beta 5 contribute to cell attachment to vitronectin but differentially distribute on the cell surface.
Cheresh DA
The Journal of cell biology 113.4 (1991 May): 919-29.