Antigen: Histone-lysine N-methyltransferase EZH2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Drosophila, Human
Depositors Notes: The protein recognized by this antibody is also known as Enhanced of zeste homolog 2.
Antigen Molecular Weight: 85.36 kDa
Human Protein Atlas:
Predicted Species Reactivity: Human, Mouse, Rat, Zebrafish
Immunogen: Recombinant Peptide
Alternate Gene Names: ENX-1, EZH1, KMT6, KMT6A
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: Q15910
Immunogen Sequence: a.a. 612-727
Entrez Gene ID: 2146
Antibody Registry ID: AB_2618615
Additional Information: RRID:AB_2618615
Recommended Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-EZH2-1B3 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-EZH2-1B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).