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View product citations for antibody PCRP-EZH2-1B3 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: Histone-lysine N-methyltransferase EZH2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Drosophila, Human
Depositors Notes: The protein recognized by this antibody is also known as Enhanced of zeste homolog 2.
Antigen Molecular Weight: 85.36 kDa
Human Protein Atlas:  
Predicted Species Reactivity: Human, Mouse, Rat, Zebrafish
Gene: EZH2
Immunogen: Recombinant Peptide
Alternate Gene Names: ENX-1, EZH1, KMT6, KMT6A
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: Q15910 
Immunogen Sequence: a.a. 612-727
Entrez Gene ID: 2146 
Additional Characterization:  
Antibody Registry ID: AB_2618615 
Additional Information: RRID:AB_2618615
Recommended Applications: Chromatin Immunoprecipitation, Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-EZH2-1B3 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-EZH2-1B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • All References
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