9E 10

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$40.00
SKU: 9E 10
View product citations for antibody 9E 10 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: c-MYC
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Bishop, J.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of California, San Francisco
Positive Tested Species Reactivity: Epitope tag/Fusion protein
Depositors Notes: Binds to protein A
Antigen Molecular Weight: 62 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: MYC
Immunogen: c-Myc (human; C-terminus a.a. 408-439) synthetic peptide.
Alternate Gene Names: MRTL; MYCC; c-Myc; bHLHe39
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: EQKLISEEDL
Uniprot ID: P01106 
Immunogen Sequence: AEEQKLISEEDLLRKRREQLKHKLEQLRNSCA; a.a. 408-432
Entrez Gene ID: 4609 
Additional Characterization:
Antibody Registry ID: AB_2266850 
Additional Information:
Recommended Applications: ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
9E 10 was deposited to the DSHB by Bishop, J.M. (DSHB Hybridoma Product 9E 10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

47 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • ELISA References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Bishop JM
    Molecular and cellular biology 5.12 (1985 Dec): 3610-6.

    IF References

    Coordinated nuclear and synaptic shuttling of afadin promotes spine plasticity and histone modifications.
    Penzes P
    The Journal of biological chemistry 289.15 (2014 Apr 11): 10831-42.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation.
    Fann MJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.12 (2010 Mar 24): 4428-39.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    Latrophilins function as heterophilic cell-adhesion molecules by binding to teneurins: regulation by alternative splicing.
    Südhof TC
    The Journal of biological chemistry 289.1 (2014 Jan 3): 387-402.

    Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
    Morello R
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 29.3 (2014 Mar): 666-75.

    GABAergic circuit dysfunction in the Drosophila Fragile X syndrome model.
    Broadie K
    Neurobiology of disease 65. (2014 May): 142-59.

    Ipsen 5i is a Novel Potent Pharmacoperone for Intracellularly Retained Melanocortin-4 Receptor Mutants.
    Huang H
    Frontiers in endocrinology 5. (2014): 131.

    Peptidergic cell-specific synaptotagmins in Drosophila: localization to dense-core granules and regulation by the bHLH protein DIMMED.
    Taghert PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.39 (2014 Sep 24): 13195-207.

    Cdk12 and Cdk13 regulate axonal elongation through a common signaling pathway that modulates Cdk5 expression.
    Fann MJ
    Experimental neurology 261. (2014 Nov): 10-21.

    Persistent replicative stress alters polycomb phenotypes and tissue homeostasis in Drosophila melanogaster.
    Jones DL
    Cell reports 7.3 (2014 May 8): 859-70.

    Neuronal nitric oxide synthase-dependent S-nitrosylation of gephyrin regulates gephyrin clustering at GABAergic synapses.
    Schwarz G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.23 (2014 Jun 4): 7763-8.

    Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 523.18 (2015 Dec 15): 2637-64.

    The heptad repeat domain 1 of Mitofusin has membrane destabilization function in mitochondrial fusion.
    Tareste D
    EMBO reports 19.6 (2018 Jun): .

    Kindlin-3 Is Essential for the Resting α4β1 Integrin-mediated Firm Cell Adhesion under Shear Flow Conditions.
    Chen J
    The Journal of biological chemistry 291.19 (2016 May 6): 10363-71.

    WB References

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Bishop JM
    Molecular and cellular biology 5.12 (1985 Dec): 3610-6.

    Prediction and validation of protein-protein interactors from genome-wide DNA-binding data using a knowledge-based machine-learning approach.
    Bouveret R
    Open biology 6.9 (2016 Sep): .

    Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
    Höhne W
    Protein engineering 14.10 (2001 Oct): 803-6.

    Identification of TgCBAP, a novel cytoskeletal protein that localizes to three distinct subcompartments of the Toxoplasma gondii pellicle.
    Ward GE
    PloS one 9.6 (2014): e98492.

    Identification and characterization of the CDK12/cyclin L1 complex involved in alternative splicing regulation.
    Fann MJ
    Molecular and cellular biology 26.7 (2006 Apr): 2736-45.

    Protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation.
    Fann MJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.12 (2010 Mar 24): 4428-39.

    ZNRF1 promotes Wallerian degeneration by degrading AKT to induce GSK3B-dependent CRMP2 phosphorylation.
    Araki T
    Nature cell biology 13.12 (2011 Nov 6): 1415-23.

    Progranulin transcripts with short and long 5' untranslated regions (UTRs) are differentially expressed via posttranscriptional and translational repression.
    Haass C
    The Journal of biological chemistry 289.37 (2014 Sep 12): 25879-89.

    Ribosome assembly factors Pwp1 and Nop12 are important for folding of 5.8S rRNA during ribosome biogenesis in Saccharomyces cerevisiae.
    Woolford JL Jr
    Molecular and cellular biology 34.10 (2014 May): 1863-77.

    Regulation of plasticity and fibrogenic activity of trabecular meshwork cells by Rho GTPase signaling.
    Rao PV
    Journal of cellular physiology 229.7 (2014 Jul): 927-42.

    Low levels of p53 protein and chromatin silencing of p53 target genes repress apoptosis in Drosophila endocycling cells.
    Calvi BR
    PLoS genetics 10.9 (2014 Sep): e1004581.

    Identification and characterization of collagen-like glycosylation and hydroxylation of CCN1.
    Simizu S
    Glycobiology 29.10 (2019 Sep 20): 696-704.

    Functional Correlation between Subcellular Localizations of Japanese Encephalitis Virus Capsid Protein and Virus Production.
    Morita E
    Journal of virology 93.19 (2019 Oct 1): .

    Src and Fyn define a new signaling cascade activated by canonical and non-canonical Wnt ligands and required for gene transcription and cell invasion.
    Duñach M
    Cellular and molecular life sciences : CMLS . (2019 Jul 16): .

    IHC References

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Syne proteins anchor muscle nuclei at the neuromuscular junction.
    Han M
    Proceedings of the National Academy of Sciences of the United States of America 102.12 (2005 Mar 22): 4359-64.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    Adeno-associated virus-mediated overexpression of LARGE rescues α-dystroglycan function in dystrophic mice with mutations in the fukutin-related protein.
    Lu QL
    Human gene therapy methods 25.3 (2014 Jun): 187-96.

    GABAergic circuit dysfunction in the Drosophila Fragile X syndrome model.
    Broadie K
    Neurobiology of disease 65. (2014 May): 142-59.

    A novel role for Lh3 dependent ECM modifications during neural crest cell migration in zebrafish.
    Granato M
    PloS one 8.1 (2013): e54609.

    Initiation of synapse formation by Wnt-induced MuSK endocytosis.
    Granato M
    Development (Cambridge, England) 139.5 (2012 Mar): 1023-33.

    Syk and Zap-70 function redundantly to promote angioblast migration.
    Childs SJ
    Developmental biology 340.1 (2010 Apr 1): 22-9.

    Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish.
    Goldman D
    Molecular and cellular neurosciences 43.4 (2010 Apr): 370-83.

    Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal.
    Brand M
    Neural development 4. (2009 Apr 2): 12.

    Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril.
    Strähle U
    The Journal of cell biology 180.6 (2008 Mar 24): 1163-75.

    Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development.
    Bach I
    Mechanisms of development 117.1-2 (2002 Sep): 75-85.

    Tcf7l2/Tcf4 Transcriptional Repressor Function Requires HDAC Activity in the Developing Vertebrate CNS.
    Matise MP
    PloS one 11.9 (2016): e0163267.

    Insight into the Etiology of Undifferentiated Soft Tissue Sarcomas from a Novel Mouse Model.
    Chiang C
    Molecular cancer research : MCR 17.5 (2019 May): 1024-1035.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    IP References

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Bishop JM
    Molecular and cellular biology 5.12 (1985 Dec): 3610-6.

    Integrin α3β1 can function to promote spontaneous metastasis and lung colonization of invasive breast carcinoma.
    Stipp CS
    Molecular cancer research : MCR 12.1 (2014 Jan): 143-154.

    Structural basis of Toxoplasma gondii MIC2-associated protein interaction with MIC2.
    Carruthers VB
    The Journal of biological chemistry 290.3 (2015 Jan 16): 1432-41.

    Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1.
    Lee JH
    The FEBS journal 281.17 (2014 Sep): 3816-27.

    ELISA References
    FACS References
    Epitope Map References
    All References

    dAcsl, the Drosophila ortholog of acyl-CoA synthetase long-chain family member 3 and 4, inhibits synapse growth by attenuating bone morphogenetic protein signaling via endocytic recycling.
    Zhang YQ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.8 (2014 Feb 19): 2785-96.

    Exocrine specific expression of Connexin32 is dependent on the basic helix-loop-helix transcription factor Mist1.
    Konieczny SF
    Journal of cell science 116.Pt 16 (2003 Aug 15): 3315-25.

    Syne proteins anchor muscle nuclei at the neuromuscular junction.
    Han M
    Proceedings of the National Academy of Sciences of the United States of America 102.12 (2005 Mar 22): 4359-64.

    Regulation of Fasciclin II and synaptic terminal development by the splicing factor beag.
    McCabe BD
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.20 (2012 May 16): 7058-73.

    Adeno-associated virus-mediated overexpression of LARGE rescues α-dystroglycan function in dystrophic mice with mutations in the fukutin-related protein.
    Lu QL
    Human gene therapy methods 25.3 (2014 Jun): 187-96.

    GABAergic circuit dysfunction in the Drosophila Fragile X syndrome model.
    Broadie K
    Neurobiology of disease 65. (2014 May): 142-59.

    A novel role for Lh3 dependent ECM modifications during neural crest cell migration in zebrafish.
    Granato M
    PloS one 8.1 (2013): e54609.

    Initiation of synapse formation by Wnt-induced MuSK endocytosis.
    Granato M
    Development (Cambridge, England) 139.5 (2012 Mar): 1023-33.

    Syk and Zap-70 function redundantly to promote angioblast migration.
    Childs SJ
    Developmental biology 340.1 (2010 Apr 1): 22-9.

    Tuba1a gene expression is regulated by KLF6/7 and is necessary for CNS development and regeneration in zebrafish.
    Goldman D
    Molecular and cellular neurosciences 43.4 (2010 Apr): 370-83.

    Zebrafish gbx1 refines the midbrain-hindbrain boundary border and mediates the Wnt8 posteriorization signal.
    Brand M
    Neural development 4. (2009 Apr 2): 12.

    Shuttling of the chaperones Unc45b and Hsp90a between the A band and the Z line of the myofibril.
    Strähle U
    The Journal of cell biology 180.6 (2008 Mar 24): 1163-75.

    Multiple functions of LIM domain-binding CLIM/NLI/Ldb cofactors during zebrafish development.
    Bach I
    Mechanisms of development 117.1-2 (2002 Sep): 75-85.

    Tcf7l2/Tcf4 Transcriptional Repressor Function Requires HDAC Activity in the Developing Vertebrate CNS.
    Matise MP
    PloS one 11.9 (2016): e0163267.

    Insight into the Etiology of Undifferentiated Soft Tissue Sarcomas from a Novel Mouse Model.
    Chiang C
    Molecular cancer research : MCR 17.5 (2019 May): 1024-1035.

    Mad1 destabilizes p53 by preventing PML from sequestering MDM2.
    Weaver BA
    Nature communications 10.1 (2019 Apr 4): 1540.

    Coordinated nuclear and synaptic shuttling of afadin promotes spine plasticity and histone modifications.
    Penzes P
    The Journal of biological chemistry 289.15 (2014 Apr 11): 10831-42.

    Regulation of Hippo signaling by Jun kinase signaling during compensatory cell proliferation and regeneration, and in neoplastic tumors.
    Irvine KD
    Developmental biology 350.1 (2011 Feb 1): 139-51.

    Protogenin defines a transition stage during embryonic neurogenesis and prevents precocious neuronal differentiation.
    Fann MJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.12 (2010 Mar 24): 4428-39.

    Latrophilins function as heterophilic cell-adhesion molecules by binding to teneurins: regulation by alternative splicing.
    Südhof TC
    The Journal of biological chemistry 289.1 (2014 Jan 3): 387-402.

    Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis.
    Morello R
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 29.3 (2014 Mar): 666-75.

    Ipsen 5i is a Novel Potent Pharmacoperone for Intracellularly Retained Melanocortin-4 Receptor Mutants.
    Huang H
    Frontiers in endocrinology 5. (2014): 131.

    Peptidergic cell-specific synaptotagmins in Drosophila: localization to dense-core granules and regulation by the bHLH protein DIMMED.
    Taghert PH
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.39 (2014 Sep 24): 13195-207.

    Cdk12 and Cdk13 regulate axonal elongation through a common signaling pathway that modulates Cdk5 expression.
    Fann MJ
    Experimental neurology 261. (2014 Nov): 10-21.

    Persistent replicative stress alters polycomb phenotypes and tissue homeostasis in Drosophila melanogaster.
    Jones DL
    Cell reports 7.3 (2014 May 8): 859-70.

    Neuronal nitric oxide synthase-dependent S-nitrosylation of gephyrin regulates gephyrin clustering at GABAergic synapses.
    Schwarz G
    The Journal of neuroscience : the official journal of the Society for Neuroscience 34.23 (2014 Jun 4): 7763-8.

    Neuropeptide F neurons modulate sugar reward during associative olfactory learning of Drosophila larvae.
    Thum AS
    The Journal of comparative neurology 523.18 (2015 Dec 15): 2637-64.

    The heptad repeat domain 1 of Mitofusin has membrane destabilization function in mitochondrial fusion.
    Tareste D
    EMBO reports 19.6 (2018 Jun): .

    Kindlin-3 Is Essential for the Resting α4β1 Integrin-mediated Firm Cell Adhesion under Shear Flow Conditions.
    Chen J
    The Journal of biological chemistry 291.19 (2016 May 6): 10363-71.

    Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
    Bishop JM
    Molecular and cellular biology 5.12 (1985 Dec): 3610-6.

    Prediction and validation of protein-protein interactors from genome-wide DNA-binding data using a knowledge-based machine-learning approach.
    Bouveret R
    Open biology 6.9 (2016 Sep): .

    Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose.
    Höhne W
    Protein engineering 14.10 (2001 Oct): 803-6.

    Identification of TgCBAP, a novel cytoskeletal protein that localizes to three distinct subcompartments of the Toxoplasma gondii pellicle.
    Ward GE
    PloS one 9.6 (2014): e98492.

    Identification and characterization of the CDK12/cyclin L1 complex involved in alternative splicing regulation.
    Fann MJ
    Molecular and cellular biology 26.7 (2006 Apr): 2736-45.

    ZNRF1 promotes Wallerian degeneration by degrading AKT to induce GSK3B-dependent CRMP2 phosphorylation.
    Araki T
    Nature cell biology 13.12 (2011 Nov 6): 1415-23.

    Progranulin transcripts with short and long 5' untranslated regions (UTRs) are differentially expressed via posttranscriptional and translational repression.
    Haass C
    The Journal of biological chemistry 289.37 (2014 Sep 12): 25879-89.

    Ribosome assembly factors Pwp1 and Nop12 are important for folding of 5.8S rRNA during ribosome biogenesis in Saccharomyces cerevisiae.
    Woolford JL Jr
    Molecular and cellular biology 34.10 (2014 May): 1863-77.

    Regulation of plasticity and fibrogenic activity of trabecular meshwork cells by Rho GTPase signaling.
    Rao PV
    Journal of cellular physiology 229.7 (2014 Jul): 927-42.

    Low levels of p53 protein and chromatin silencing of p53 target genes repress apoptosis in Drosophila endocycling cells.
    Calvi BR
    PLoS genetics 10.9 (2014 Sep): e1004581.

    Identification and characterization of collagen-like glycosylation and hydroxylation of CCN1.
    Simizu S
    Glycobiology 29.10 (2019 Sep 20): 696-704.

    Functional Correlation between Subcellular Localizations of Japanese Encephalitis Virus Capsid Protein and Virus Production.
    Morita E
    Journal of virology 93.19 (2019 Oct 1): .

    Src and Fyn define a new signaling cascade activated by canonical and non-canonical Wnt ligands and required for gene transcription and cell invasion.
    Duñach M
    Cellular and molecular life sciences : CMLS . (2019 Jul 16): .

    Integrin α3β1 can function to promote spontaneous metastasis and lung colonization of invasive breast carcinoma.
    Stipp CS
    Molecular cancer research : MCR 12.1 (2014 Jan): 143-154.

    Structural basis of Toxoplasma gondii MIC2-associated protein interaction with MIC2.
    Carruthers VB
    The Journal of biological chemistry 290.3 (2015 Jan 16): 1432-41.

    Sestrin2 promotes Unc-51-like kinase 1 mediated phosphorylation of p62/sequestosome-1.
    Lee JH
    The FEBS journal 281.17 (2014 Sep): 3816-27.

    Replacement of the essential Dictyostelium Arp2 gene by its Entamoeba homologue using parasexual genetics.
    Insall RH
    BMC genetics 8. (2007 Jun 6): 28.

    Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms.
    Wyllie AH
    The Journal of pathology 160.4 (1990 Apr): 287-93.

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