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View product citations for antibody PCRP-LHX2-1C11 on CiteAb

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Available: 37

DSHB Data Sheet

Catalog Fields

Antigen: LIM homeobox 2; LHX2
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Common Fund – Protein Capture Reagents Program
Isotype: MIgG2a
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Chick, Human
Depositors Notes:
Antigen Molecular Weight: 44.37 kDa
Human Protein Atlas:  
Predicted Species Reactivity: Human, Mouse, Rat
Gene: LHX2
Immunogen: Recombinant peptide
Alternate Gene Names: hLhx2, LH-2
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence: a.a. 231-406
Uniprot ID: P50458 
Immunogen Sequence: a.a. 231-406
Entrez Gene ID: 9355 
Additional Characterization:  
Antibody Registry ID: AB_2618817 
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program RRID: AB_2618817
Recommended Applications: Immunoprecipitation, Microarray
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-LHX2-1C11 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-LHX2-1C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication
    IHC References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    All References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

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