PCRP-LHX2-1C11

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SKU: PCRP-LHX2-1C11

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DSHB Data Sheet

Catalog Fields

Product Name/ID: PCRP-LHX2-1C11
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: LHX2
Ab Isotype: MIgG2a
Gene Symbol:
Antibody Registry ID: AB_2618817 
Uniprot ID: P50458 
RRID:  
Entrez Gene ID: 9355 
Clonality: Monoclonal
Immunogen: Recombinant peptide
Clone:
Immunogen Sequence: a.a. 231-406
Myeloma Strain: SP2/0-Ag14
Epitope Mapped: Yes
Antigen Name: LIM homeobox 2; LHX2
Epitope Location or Sequence: a.a. 231-406
Alternate Antigen Name:
Deposit Date: 12/3/2014
Antigen Molecular Weight: 44.37 kDa
Depositor: Common Fund – Protein Capture Reagents Program
Antigen Sequence:
Depositor Institution: National Institutes of Health
Antigen Species: Human
Depositor Notes:
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chick, Human
Additional Information: This antibody has been characterized by the NIH Protein Capture Reagents Program RRID: AB_2618817
Predicted Species Reactivity: Human, Mouse, Rat
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunoprecipitation, Microarray
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-LHX2-1C11 was deposited to the DSHB by Common Fund – Protein Capture Reagents Program (DSHB Hybridoma Product PCRP-LHX2-1C11)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

2 References

  • Initial Publication
  • IHC References
  • All References
  • Initial Publication
    IHC References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    All References

    Axon guidance at the spinal cord midline-A live imaging perspective.
    Stoeckli ET
    The Journal of comparative neurology 529.10 (2021 Jul 1): 2517-2538.

    A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors.
    Blackshaw S
    Nature methods 15.5 (2018 May): 330-338.

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