PCRP-SOX9-1A2

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$40.00
SKU: PCRP-SOX9-1A2
View product citations for antibody PCRP-SOX9-1A2 on CiteAb

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Available: 129

DSHB Data Sheet

Catalog Fields

Antigen: Transcription factor SOX-9
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Protein Capture Reagents Program, produced by JHU/CDI
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: National Institutes of Health
Positive Tested Species Reactivity: Human
Depositors Notes: This antibody recognizes a transcription factor that is important in skeletal development.
Antigen Molecular Weight: 56.14
Human Protein Atlas:
Predicted Species Reactivity: Human, Mouse, Rat
Gene: SOX9
Immunogen: Recombinant protein
Alternate Gene Names: CMD1, CMPD1, SRA1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0-Ag14
Epitope Location or Sequence:
Uniprot ID: P48436 
Immunogen Sequence: Protein Domain
Entrez Gene ID: 6662 
Additional Characterization:
Antibody Registry ID: AB_2619127 
Additional Information: The PCRP-SOX9-1A2 has been used successfully for western blot at 0.1 ug/ml. RRID: AB_2619127
Recommended Applications: Immunoprecipitation, Microarray, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
PCRP-SOX9-1A2 was deposited to the DSHB by Protein Capture Reagents Program, produced by JHU/CDI (DSHB Hybridoma Product PCRP-SOX9-1A2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • IF References
  • FFPE References
  • All References
  • Initial Publication
    IF References

    Estrogen Signaling Drives Ciliogenesis in Human Endometrial Organoids.
    Latos PA
    Endocrinology 160.10 (2019 Oct 1): 2282-2297.

    FFPE References

    Estrogen Signaling Drives Ciliogenesis in Human Endometrial Organoids.
    Latos PA
    Endocrinology 160.10 (2019 Oct 1): 2282-2297.

    All References

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