R5.21C
Catalog Fields
Antigen: connexin 32
Hybridoma Cells Available: Yes
Antigen Species: mouse
Depositor: Goodenough, D.A.
Isotype: RIgG2a
Antigen Sequence:
Host Species: rat
Depositors Institution: Harvard Medical School
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes: Integral membrane protein. AA sequence note: See Paul, D.L., 1986, J. Cell Biol. 103:123 for complete aa sequence. IHC note: stains cytoplasmic surfaces of gap junctions between liver cells, exocrine pancreas, stomach and others bearing connexin32.
Predicted Species Reactivity:
Gene:
Immunogen: DOC-JR-plasma membranes
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3x63 Ag8U.1 (CRL 1597)
Epitope Location or Sequence:
Antibody Registry ID: AB_2110766
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
R5.21C was deposited to the DSHB by Goodenough, D.A. (DSHB Hybridoma Product R5.21C)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Identification of ZO-1: a high molecular weight polypeptide associated with the tight junction (zonula occludens) in a variety of epithelia.
Goodenough DA
The Journal of cell biology 103.3 (1986 Sep): 755-66.