Antigen: RIM, UNC-10
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G.
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: The antibody works poorly in detecting UNC-10 on immunoblots [PMID: 20405020]. Specific to UNC-10 except that some faint staining was observed in the pharynx of unc-10 mutant [md1117]. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was restricted to discrete puncta (active zones) in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord.
Antigen Molecular Weight: Predicted: two isoforms, 175 and 155 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged UNC-10 (aa 1-144) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 4A5
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 1-144
Uniprot ID: Q22366
Immunogen Sequence: Partial protein
Entrez Gene ID: 180990
Antibody Registry ID: AB_579790
Additional Information: The Rab-3-interacting molecule unc-10 (Rim) localizes to the periphery of the active zone in neurons.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RIM was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. (DSHB Hybridoma Product RIM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Epitope Map References