Antigen: RIM, UNC-10
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G.
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: The antibody works poorly in detecting UNC-10 on immunoblots [PMID: 20405020]. Specific to UNC-10 except that some faint staining was observed in the pharynx of unc-10 mutant [md1117]. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was restricted to discrete puncta (active zones) in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord.
Predicted Species Reactivity:
Immunogen: Recombinant His6-tagged UNC-10 (aa 1-144) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 4A5
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 1-144
Uniprot ID: Q22366
Immunogen Sequence: Partial protein
Entrez Gene ID: 180990
Antibody Registry ID: AB_579790
Additional Information: The Rab-3-interacting molecule unc-10 (Rim) localizes to the periphery of the active zone in neurons.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RIM was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. (DSHB Hybridoma Product RIM)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Epitope Map References