SB1

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SKU: SB1

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DSHB Data Sheet

Catalog Fields

Product Name/ID: SB1
Available to For-Profits: Yes
Alternate Antibody Name: 5D1
Gene Name: snb-1
Ab Isotype: MIgG1
Gene Symbol:
Antibody Registry ID: AB_579792 
Uniprot ID: O02495 
RRID:  
Entrez Gene ID: 266648 
Clonality: Monoclonal
Immunogen: Recombinant His6-tagged SNB-1 (aa 13-88) fusion protein
Clone:
Immunogen Sequence: Partial protein
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Antigen Name: synaptobrevin-1
Epitope Location or Sequence: cytoplasmic (aa 17-26)
Alternate Antigen Name:
Deposit Date: 6/21/2006
Antigen Molecular Weight: Predicted: 12 kDa; Apparent: 16 kDa
Depositor: Nonet, M.L. / Hadwiger, G.
Antigen Sequence:
Depositor Institution: Washington University Medical School
Antigen Species: C. elegans
Depositor Notes: Specific to SNB-1; by western blot, the wild type band was absent and a higher MW band of reduced intensity was observed in snb-1 mutant [md247]. Synaptobrevin is localized primarily to synaptic vesicles. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was seen in the nerve ring, pharyngeal nervous system, and the ventral and dorsal nerve cords.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: C. elegans
Additional Information: SNB-1 is also known as VAMP1. SNB-1 is a neuronal v-SNARE synaptobrevin (VAMP) which is localized primarily to synaptic vesicles in C. elegans
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SB1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. (DSHB Hybridoma Product SB1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density.
    Richmond JE
    Neuroscience letters 444.2 (2008 Oct 24): 137-42.

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    IP References

    Targeted purification of SnAvi-tagged proteins.
    Schulze E
    Methods in molecular biology (Clifton, N.J.) 1177. (2014): 163-74.

    Epitope Map References

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density.
    Richmond JE
    Neuroscience letters 444.2 (2008 Oct 24): 137-42.

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    Targeted purification of SnAvi-tagged proteins.
    Schulze E
    Methods in molecular biology (Clifton, N.J.) 1177. (2014): 163-74.

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