SB1

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$40.00
SKU: SB1
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DSHB Data Sheet

Catalog Fields

Antigen: synaptobrevin-1
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: Specific to SNB-1; by western blot, the wild type band was absent and a higher MW band of reduced intensity was observed in snb-1 mutant [md247]. Synaptobrevin is localized primarily to synaptic vesicles. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was seen in the nerve ring, pharyngeal nervous system, and the ventral and dorsal nerve cords.
Antigen Molecular Weight: Predicted: 12 kDa; Apparent: 16 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: snb-1
Immunogen: Recombinant His6-tagged SNB-1 (aa 13-88) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 5D1
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: cytoplasmic (aa 17-26)
Uniprot ID: O02495 
Immunogen Sequence: Partial protein
Entrez Gene ID: 266648 
Additional Characterization:  
Antibody Registry ID: AB_579792 
Additional Information: SNB-1 is also known as VAMP1. SNB-1 is a neuronal v-SNARE synaptobrevin (VAMP) which is localized primarily to synaptic vesicles in C. elegans
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
SB1 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. (DSHB Hybridoma Product SB1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    WB References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density.
    Richmond JE
    Neuroscience letters 444.2 (2008 Oct 24): 137-42.

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    IP References

    Targeted purification of SnAvi-tagged proteins.
    Schulze E
    Methods in molecular biology (Clifton, N.J.) 1177. (2014): 163-74.

    Epitope Map References

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    All References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    Direct interactions between C. elegans RAB-3 and Rim provide a mechanism to target vesicles to the presynaptic density.
    Richmond JE
    Neuroscience letters 444.2 (2008 Oct 24): 137-42.

    SnAvi--a new tandem tag for high-affinity protein-complex purification.
    Schulze E
    Nucleic acids research 38.6 (2010 Apr): e91.

    Targeted purification of SnAvi-tagged proteins.
    Schulze E
    Methods in molecular biology (Clifton, N.J.) 1177. (2014): 163-74.

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