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DSHB Data Sheet

Catalog Fields

Antigen: RIM, UNC-10
Hybridoma Cells Available: Yes
Antigen Species: C. elegans
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Washington University Medical School
Positive Tested Species Reactivity: C. elegans
Depositors Notes: The antibody works poorly in detecting UNC-10 on immunoblots [PMID: 20405020]. Specific to UNC-10, no staining was observed in unc-10 mutant [md1117]. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was restricted to discrete puncta (active zones) in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord.
Antigen Molecular Weight: Predicted: two isoforms, 175 and 155 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: unc-10
Immunogen: Recombinant His6-tagged UNC-10 (aa 1-144) fusion protein
Alternate Gene Names:
Alternate Antibody Name: 4E11
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3X63Ag8.653
Epitope Location or Sequence: aa 1-144
Uniprot ID: Q22366 
Immunogen Sequence: Partial protein
Entrez Gene ID: 180990 
Additional Characterization:  
Antibody Registry ID: AB_10570332 
Additional Information: The Rab-3-interacting molecule unc-10 (Rim) localizes to the periphery of the active zone in neurons.
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RIM2 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product RIM2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    IHC References

    Expansion microscopy of <i>C. elegans</i>.
    Boyden ES
    eLife 9. (2020 May 1): .

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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