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View product citations for antibody RIM2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: RIM, UNC-10
Available to For-Profits: Yes
Antigen Species: C. elegans
Hybridoma Cells Available (Non-Profit): Yes
Isotype: MIgG1
Depositor: Nonet, M.L. / Hadwiger, G. / Dour, S.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: C. elegans
Depositors Institution: Washington University Medical School
Antigen Molecular Weight: Predicted: two isoforms, 175 and 155 kDa
Depositors Notes: The antibody works poorly in detecting UNC-10 on immunoblots [PMID: 20405020]. Specific to UNC-10, no staining was observed in unc-10 mutant [md1117]. By immunofluorescence microscopy on whole mount C. elegans fixed with methanol/acetone, staining was restricted to discrete puncta (active zones) in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Recombinant His6-tagged UNC-10 (aa 1-144) fusion protein
Gene: unc-10
Alternate Antibody Name: 4E11
Alternate Gene Names:
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8.653
Epitope Mapped: Yes
Uniprot ID: Q22366 
Epitope Location or Sequence: aa 1-144
Entrez Gene ID: 180990 
Immunogen Sequence: Partial protein
Antibody Registry ID: AB_10570332 
Additional Characterization:  
Recommended Applications: Immunofluorescence
Additional Information: The Rab-3-interacting molecule unc-10 (Rim) localizes to the periphery of the active zone in neurons.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
RIM2 was deposited to the DSHB by Nonet, M.L. / Hadwiger, G. / Dour, S. (DSHB Hybridoma Product RIM2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • IHC References
  • Epitope Map References
  • All References
  • Initial Publication

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    IF References
    IHC References

    Expansion microscopy of C. elegans.
    Boyden ES
    eLife 9. (2020 May 1): .

    Epitope Map References

    A monoclonal antibody toolkit for C. elegans.
    Nonet ML
    PloS one 5.4 (2010 Apr 13): e10161.

    All References

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