Catalog Fields
Antigen: Myosin light chain 1 slow, 2 slow,1 fast and 2 fast (LC1s, LC1f, LC2s, LC2f)
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Stockdale, F.E.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Stanford University School of Medicine
Positive Tested Species Reactivity: Avian, Mammal
Depositors Notes: IHC note: works well on Western blots; works well on ethanol-fixed tissue.
Predicted Species Reactivity:
Gene:
Immunogen: myosin isolated from chicken pectoralis major muscle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3/NS1.1Ag4-1
Epitope Location or Sequence:
Antibody Registry ID: AB_531865
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
T14 was deposited to the DSHB by Stockdale, F.E. (DSHB Hybridoma Product T14)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
1 Reference
Initial Publication
Myosin expression and specialization among the earliest muscle fibers of the developing avian limb.
Stockdale FE
Developmental biology 113.1 (1986 Jan): 238-54.