(0)No Reviews yet
1 – 19$50.00
20 – 49$35.00
50 – 99$32.00
100 +$30.00
SKU: TRA-2-49/6E-s

In Stock

Available: 36

DSHB Data Sheet

Catalog Fields

Clone ID/Product Name: TRA-2-49/6E
Available to For-Profits: Yes
Alternate Antibody Name: chromosome 1, 80 kDa polypeptide
Gene Symbol: ALPL
Ab Isotype: MIgG1
Gene Name:
Antibody Registry ID: AB_528073 
Uniprot ID: P05186 
Entrez Gene ID: 249 
Clonality: Monoclonal
Immunogen: Embryonal carcinoma (EC) 2102Ep cells
Immunogen Sequence: Full length sequence
Myeloma Strain: P3X63Ag8-653
Epitope Mapped: No
Antigen Name: alkaline phosphatase, tissue non-specific isoenzyme
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 12/11/1990
Antigen Molecular Weight: ~80 kDa
Depositor: Andrews, P.W.
Antigen Sequence:
Depositor Institution: University of Sheffield
Antigen Species: Human
Depositor Notes: This antibody recognizes alkaline phosphatase isozyme from liver, bone and kidney but does not recognize intestinal or placental alkaline phosphatases.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): No
Confirmed Species Reactivity: Human, Marmoset, Non-human primates, Squirrel monkey
Additional Information: RRID:AB_528073; This antibody does not react with mouse ALP.
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TRA-2-49/6E was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product TRA-2-49/6E)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    FACS References
    All References

    Ratings & Reviews

    No reviews available

    Be the first to Write a Review