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SKU: TRA-2-49/6E-s
View product citations for antibody TRA-2-49/6E on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: alkaline phosphatase, tissue non-specific isoenzyme
Available to For-Profits: Yes
Antigen Species: Human
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG1
Depositor: Andrews, P.W.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Human, Marmoset, Non-human primates, Squirrel monkey
Depositors Institution: University of Sheffield
Antigen Molecular Weight: ~80 kDa
Depositors Notes: This antibody recognizes alkaline phosphatase isozyme from liver, bone and kidney but does not recognize intestinal or placental alkaline phosphatases.
Predicted Species Reactivity:  
Human Protein Atlas:  
Immunogen: Embryonal carcinoma (EC) 2102Ep cells
Gene: ALPL
Alternate Antibody Name: chromosome 1, 80 kDa polypeptide
Alternate Gene Names: HOPS, TNSALP
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: P3X63Ag8-653
Epitope Mapped: No
Uniprot ID: P05186 
Epitope Location or Sequence:
Entrez Gene ID: 249 
Immunogen Sequence: Full length sequence
Antibody Registry ID: AB_528073 
Additional Characterization:  
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Additional Information: RRID:AB_528073; This antibody does not react with mouse ALP.
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
TRA-2-49/6E was deposited to the DSHB by Andrews, P.W. (DSHB Hybridoma Product TRA-2-49/6E)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • ELISA References
  • FACS References
  • All References
  • Initial Publication
    IF References
    WB References
    IP References
    ELISA References
    FACS References
    All References

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