MC-813-70 (SSEA-4)

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SKU: MC-813-70 (SSEA-4)
View product citations for antibody MC-813-70 (SSEA-4) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SSEA-4/ stage-specific embryonic antigen 4/globolipid
Depositor: Solter, D. / Knowles, B.B
Antigen Species: Human
Depositors Institution: A*STAR Institute of Medical Biology
Isotype: MIgG3
Depositors Notes: This antibody recognizes a defined carbohydrate epitope on a glycolipid expressed by human embryonic carcinoma and embryonic stem cells.
Host Species: mouse
Clonality: Monoclonal
Positive Tested Species Reactivity: Canine, Cat, Chicken, Equine, Human, Mouse, Non-human primates, Rabbit, Rat
Epitope Mapped: Yes
Immunogen: Human embryonal carcinoma 2102Ep cells
Epitope Location or Sequence: Carbohydrate moiety of glycolipid/ NeuAc alpha2- 3Gal beta1- 3GalNAc beta1
Myeloma Strain: SP2/0
Immunogen Sequence: Intact cells
Antibody Registry ID: AB_528477 
Additional Information: RRID:AB_528477
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MC-813-70 (SSEA-4) was deposited to the DSHB by Solter, D. / Knowles, B.B (DSHB Hybridoma Product MC-813-70 (SSEA-4))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    FACS References
    Epitope Map References
    All References

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