MC-813-70 (SSEA-4)

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SKU: MC-813-70 (SSEA-4)
View product citations for antibody MC-813-70 (SSEA-4) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: SSEA-4/ stage-specific embryonic antigen 4/globolipid
Hybridoma Cells Available: No
Antigen Species: Human
Depositor: Solter, D. / Knowles, B.B
Isotype: MIgG3
Antigen Sequence:
Host Species: mouse
Depositors Institution: A*STAR Institute of Medical Biology
Positive Tested Species Reactivity: Canine, Cat, Chicken, Equine, Human, Mouse, Non-human primates, Rabbit, Rat
Depositors Notes: This antibody recognizes a defined carbohydrate epitope on a glycolipid expressed by human embryonic carcinoma and embryonic stem cells.
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: Human embryonal carcinoma 2102Ep cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2/0
Epitope Location or Sequence: Carbohydrate moiety of glycolipid/ NeuAc alpha2- 3Gal beta1- 3GalNAc beta1
Uniprot ID:  
Immunogen Sequence: Intact cells
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528477 
Additional Information: RRID:AB_528477
Recommended Applications: ELISA, FACS, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
MC-813-70 (SSEA-4) was deposited to the DSHB by Solter, D. / Knowles, B.B (DSHB Hybridoma Product MC-813-70 (SSEA-4))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

7 References

  • Initial Publication
  • IF References
  • IHC References
  • FACS References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References
    IHC References
    FACS References
    Epitope Map References
    All References

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