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SKU: VA1(3)

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DSHB Data Sheet

Catalog Fields

Product Name/ID: VA1(3)
Available to For-Profits: Yes
Alternate Antibody Name: VA1-3, VA1 3, VA13
Gene Name: FN1
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2105709 
Uniprot ID: F1NJT3 
Entrez Gene ID: 396133 
Clonality: Monoclonal
Immunogen: Undifferentiated mesenchyme and ectodermal sleeve from chicken wing bud (stage 23-24)
Immunogen Sequence: Full length protein
Myeloma Strain: NS1
Epitope Mapped: No
Antigen Name: fibronectin
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 4/16/1986
Antigen Molecular Weight: Predicted: 270 kDa; Apparent: multiple isoforms around 230 kDa
Depositor: Solursh, M.
Antigen Sequence:
Depositor Institution: University of Iowa
Antigen Species: chicken
Depositor Notes: Doesn't cross react with rat or frog fibronectin. VA1(3) recognizes intact FN as well as the 140/160 kDa cell binding fragments generated by elastase digestion.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Bovine, Chicken, Newt
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VA1(3) was deposited to the DSHB by Solursh, M. (DSHB Hybridoma Product VA1(3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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