VA1(3)

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$40.00
SKU: VA1(3)
View product citations for antibody VA1(3) on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: fibronectin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Solursh, M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Iowa
Positive Tested Species Reactivity: Bovine, Chicken, Newt
Depositors Notes: Doesn't cross react with rat or frog fibronectin. VA1(3) recognizes intact FN as well as the 140/160 kDa cell binding fragments generated by elastase digestion.
Antigen Molecular Weight: Predicted: 270 kDa; Apparent: multiple isoforms around 230 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: FN1
Immunogen: Undifferentiated mesenchyme and ectodermal sleeve from chicken wing bud (stage 23-24)
Alternate Gene Names: FN
Alternate Antibody Name: VA1-3, VA1 3, VA13
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID: F1NJT3 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396133 
Additional Characterization:  
Antibody Registry ID: AB_2105709 
Additional Information:
Recommended Applications: Immunofluorescence, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
VA1(3) was deposited to the DSHB by Solursh, M. (DSHB Hybridoma Product VA1(3))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

4 References

  • Initial Publication
  • IF References
  • WB References
  • All References
  • Initial Publication
    IF References
    WB References
    All References

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