Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG2a, kappa light chain
Host Species: mouse
Depositors Institution: G.V. Montgomery VA Medical Center
Positive Tested Species Reactivity: Mouse, Rat
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: THEKGAHDVPFPKTEEV+C (KLH)
Alternate Gene Names: Mlr, MCR
Alternate Antibody Name: rMR 365–381 4D6
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 365-381)
Uniprot ID: P22199
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672
Antibody Registry ID: AB_2267538
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 365–381 peptide is identical between rat and mouse, but differs in human.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR365 4D6 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR365 4D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
Endocrinology 147.3 (2006 Mar): 1343-8.
Alvarez de la Rosa D
Endocrinology 157.6 (2016 Jun): 2515-32.
Epitope Map References