rMR365 4D6

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SKU: rMR365 4D6
View product citations for antibody rMR365 4D6 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: mineralocorticoid receptor
Hybridoma Cells Available: Yes
Antigen Species: Rat
Depositor: Gomez-Sanchez, C.
Isotype: MIgG2a, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: G.V. Montgomery VA Medical Center
Positive Tested Species Reactivity: Mouse, Rat
Depositors Notes:
Antigen Molecular Weight: Predicted: 107 kDa; Apparent: 107 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: Nr3c2
Alternate Gene Names: Mlr, MCR
Alternate Antibody Name: rMR 365–381 4D6
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: SP2
Epitope Location or Sequence: N-terminus A/B domain (aa 365-381)
Uniprot ID: P22199 
Immunogen Sequence: Partial protein
Entrez Gene ID: 25672 
Additional Characterization:  
Antibody Registry ID: AB_2267538 
Additional Information: Should recognize all MR isoforms. The sequence of the rMR 365–381 peptide is identical between rat and mouse, but differs in human.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
rMR365 4D6 was deposited to the DSHB by Gomez-Sanchez, C. (DSHB Hybridoma Product rMR365 4D6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • WB References
  • IHC References
  • Epitope Map References
  • All References
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