Antigen: notochord marker
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Host Species: mouse
Depositors Institution: Center for Neurobiology & Behavior, Columbia University
Positive Tested Species Reactivity: Chicken
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:
Immunogen: notochord--homogenized tissue
Alternate Gene Names:
Alternate Antibody Name:
Alternate Antigen Name:
Myeloma Strain: Designation of NS-1
Epitope Location or Sequence:
Entrez Gene ID:
Antibody Registry ID: AB_531881
Additional Information: Immunization of the antibody was on 04/14/89; 04/28/89; 05/15/89; 06/04/89 with 10 notochords of antigen. Adjuvant of the immunization was complete Freund’s adjuvant followed by incomplete Freund’s adjuvant. The origin of the antibody was from dissected tissue of a chick. RRID:AB_531881
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15.3B9(NOT1) was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 15.3B9(NOT1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).