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SKU: 15.3B9(NOT1)
View product citations for antibody 15.3B9(NOT1) on CiteAb

In Stock

Available: 17

DSHB Data Sheet

Catalog Fields

Antigen: notochord marker
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Jessell, T.M. / Dodd, J. / Brenner-Morton, S.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Center for Neurobiology & Behavior, Columbia University
Positive Tested Species Reactivity: Chicken
Depositors Notes:
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Immunogen: notochord--homogenized tissue
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Designation of NS-1
Epitope Location or Sequence:
Uniprot ID:
Immunogen Sequence:
Entrez Gene ID:
Additional Characterization:
Antibody Registry ID: AB_531881 
Additional Information: Immunization of the antibody was on 04/14/89; 04/28/89; 05/15/89; 06/04/89 with 10 notochords of antigen. Adjuvant of the immunization was complete Freund’s adjuvant followed by incomplete Freund’s adjuvant. The origin of the antibody was from dissected tissue of a chick. RRID:AB_531881
Recommended Applications: Immunofluorescence
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
15.3B9(NOT1) was deposited to the DSHB by Jessell, T.M. / Dodd, J. / Brenner-Morton, S. (DSHB Hybridoma Product 15.3B9(NOT1))
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

3 References

  • Initial Publication
  • All References
  • Initial Publication

    Jessell TM
    Cell 64.3 (1991 Feb 8): 635-47.

    All References

    Jessell TM
    Cell 64.3 (1991 Feb 8): 635-47.

    Experimental cell research
    Lash JW
    Experimental cell research 211.2 (1994 Apr): 263-74.

    Developmental biology
    George-Weinstein M
    Developmental biology 359.1 (2011 Nov 1): 12-25.

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