1D10

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$40.00
SKU: 1D10
View product citations for antibody 1D10 on CiteAb

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Available: 160

DSHB Data Sheet

Catalog Fields

Antigen: CD44
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Halfter, W.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Neurobiology, University of Pittsburgh
Positive Tested Species Reactivity: Chicken, Quail
Depositors Notes: Western blotting works under non-reducing conditions. Labels macrophages in Chicken and quail as well as optic nerve.
Antigen Molecular Weight: Multiple isoforms
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: CD44
Immunogen: proteoglycans from E10 Chicken embryos
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: Ag8
Epitope Location or Sequence:
Uniprot ID: F1NL98 
Immunogen Sequence: Full length protein
Entrez Gene ID: 395666 
Additional Characterization:
Antibody Registry ID: AB_2244424 
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D10 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 1D10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology and visual science 46.3 (2005 Mar): 1000-9.

    IF References

    Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.
    Vautherot JF
    Stem cell research 14.2 (2015 Mar): 224-37.

    Versican knockdown reduces interzone area during early stages of chick synovial joint development.
    Capehart AA
    Anatomical record (Hoboken, N.J. : 2007) 295.3 (2012 Mar): 397-409.

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology and visual science 46.3 (2005 Mar): 1000-9.

    WB References

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    IHC References

    Atypical molecular profile for joint development in the avian costal joint.
    Burke AC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.10 (2010 Oct): 2547-57.

    All References

    Atypical molecular profile for joint development in the avian costal joint.
    Burke AC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.10 (2010 Oct): 2547-57.

    Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.
    Vautherot JF
    Stem cell research 14.2 (2015 Mar): 224-37.

    Versican knockdown reduces interzone area during early stages of chick synovial joint development.
    Capehart AA
    Anatomical record (Hoboken, N.J. : 2007) 295.3 (2012 Mar): 397-409.

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology and visual science 46.3 (2005 Mar): 1000-9.

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