1D10

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SKU: 1D10

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DSHB Data Sheet

Catalog Fields

Product Name/ID: 1D10
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CD44
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Antibody Registry ID: AB_2244424 
Uniprot ID: F1NL98 
RRID:  
Entrez Gene ID: 395666 
Clonality: Monoclonal
Immunogen: proteoglycans from E10 Chicken embryos
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: Ag8
Epitope Mapped: No
Antigen Name: CD44
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 3/30/2004
Antigen Molecular Weight: Multiple isoforms
Depositor: Halfter, W.M.
Antigen Sequence:
Depositor Institution: University of Pittsburgh
Antigen Species: chicken
Depositor Notes: Western blotting works under non-reducing conditions. Labels macrophages in Chicken and quail as well as optic nerve.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Quail
Additional Information:
Predicted Species Reactivity:  
Human Protein Atlas:  
Additional Characterization:  
Recommended Applications: Immunofluorescence, Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1D10 was deposited to the DSHB by Halfter, W.M. (DSHB Hybridoma Product 1D10)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

5 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • All References
  • Initial Publication

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology & visual science 46.3 (2005 Mar): 1000-9.

    IF References

    Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.
    Vautherot JF
    Stem cell research 14.2 (2015 Mar): 224-37.

    Versican knockdown reduces interzone area during early stages of chick synovial joint development.
    Capehart AA
    Anatomical record (Hoboken, N.J. : 2007) 295.3 (2012 Mar): 397-409.

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology & visual science 46.3 (2005 Mar): 1000-9.

    WB References

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    IHC References

    Atypical molecular profile for joint development in the avian costal joint.
    Burke AC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.10 (2010 Oct): 2547-57.

    All References

    Atypical molecular profile for joint development in the avian costal joint.
    Burke AC
    Developmental dynamics : an official publication of the American Association of Anatomists 239.10 (2010 Oct): 2547-57.

    Derivation of keratinocytes from chicken embryonic stem cells: establishment and characterization of differentiated proliferative cell populations.
    Vautherot JF
    Stem cell research 14.2 (2015 Mar): 224-37.

    Versican knockdown reduces interzone area during early stages of chick synovial joint development.
    Capehart AA
    Anatomical record (Hoboken, N.J. : 2007) 295.3 (2012 Mar): 397-409.

    Cells activated for wound repair have the potential to direct collective invasion of an epithelium.
    Walker JL
    Molecular biology of the cell 27.3 (2016 Feb 1): 451-65.

    Basement membrane-dependent survival of retinal ganglion cells.
    Mayer U
    Investigative ophthalmology & visual science 46.3 (2005 Mar): 1000-9.

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