6B3

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$40.00
SKU: 6B3
View product citations for antibody 6B3 on CiteAb

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Available: 54

DSHB Data Sheet

Catalog Fields

Antigen: Cadherin, N- (neural)
Hybridoma Cells Available: Yes
Antigen Species: Chicken
Depositor: Knudsen, K.A.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: Lankenau Institute for Medical Research
Positive Tested Species Reactivity: Chicken, Zebrafish
Depositors Notes: This antibody does not recognize other cadherins including R-cadherin or mouse N-cadherin.
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 135 kDa (full length), 100-kDa (extracellular fragment)
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: CDH2
Immunogen: Day 11 chick embryo heart extract
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: P3/NSI/1-AG4-1
Epitope Location or Sequence: The antibody binds the extracellular domain of N-cadherin.
Uniprot ID: P10288 
Immunogen Sequence: Affinity purified N-cadherin/catenin complex
Entrez Gene ID: 414745 
Additional Characterization:  
Antibody Registry ID: AB_528118 
Additional Information: RRID:AB_528118
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B3 was deposited to the DSHB by Knudsen, K.A. (DSHB Hybridoma Product 6B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • FB References
  • All References
  • Initial Publication
    IF References

    N-cadherin promotes the commitment and differentiation of skeletal muscle precursor cells.
    Knudsen KA
    Developmental biology 185.1 (1997 May 1): 14-24.

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production.
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Integration of left-right Pitx2 transcription and Wnt signaling drives asymmetric gut morphogenesis via Daam2.
    Kurpios NA
    Developmental cell 26.6 (2013 Sep 30): 629-44.

    Notch1 signaling regulates chondrogenic lineage determination through Sox9 activation.
    Rohwedel J
    Cell death and differentiation 19.3 (2012 Mar): 461-9.

    Notch1 signaling regulates chondrogenic lineage determination through Sox9 activation.
    Rohwedel J
    Cell death and differentiation 19.3 (2012 Mar): 461-9.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

    Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse.
    Heuckeroth RO
    Developmental biology 409.2 (2016 Jan 15): 473-88.

    WB References
    IHC References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production.
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Cerebral organoids model human brain development and microcephaly.
    Knoblich JA
    Nature 501.7467 (2013 Sep 19): 373-9.

    Axon sorting within the spinal cord marginal zone via Robo-mediated inhibition of N-cadherin controls spinocerebellar tract formation.
    Kaprielian Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.44 (2012 Oct 31): 15377-87.

    PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Weijer CJ
    Development (Cambridge, England) 135.21 (2008 Nov): 3521-30.

    Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse.
    Heuckeroth RO
    Developmental biology 409.2 (2016 Jan 15): 473-88.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.
    Bronner ME
    Mechanisms of development 152. (2018 Aug): 44-56.

    IP References
    FB References
    All References

    Immunophenotypic characterization of enteric neural crest cells in the developing avian colorectum.
    Goldstein AM
    Developmental dynamics : an official publication of the American Association of Anatomists 241.5 (2012 May): 842-51.

    Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production.
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Cerebral organoids model human brain development and microcephaly.
    Knoblich JA
    Nature 501.7467 (2013 Sep 19): 373-9.

    Axon sorting within the spinal cord marginal zone via Robo-mediated inhibition of N-cadherin controls spinocerebellar tract formation.
    Kaprielian Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 32.44 (2012 Oct 31): 15377-87.

    PDGF signalling controls the migration of mesoderm cells during chick gastrulation by regulating N-cadherin expression.
    Weijer CJ
    Development (Cambridge, England) 135.21 (2008 Nov): 3521-30.

    Ibuprofen slows migration and inhibits bowel colonization by enteric nervous system precursors in zebrafish, chick and mouse.
    Heuckeroth RO
    Developmental biology 409.2 (2016 Jan 15): 473-88.

    Effects of Antitumor Drug Sorafenib on Chick Embryo Development.
    Yang XS
    Anatomical record (Hoboken, N.J. : 2007) 298.7 (2015 Jul): 1271-81.

    A catenin-dependent balance between N-cadherin and E-cadherin controls neuroectodermal cell fate choices.
    Bronner ME
    Mechanisms of development 152. (2018 Aug): 44-56.

    N-cadherin promotes the commitment and differentiation of skeletal muscle precursor cells.
    Knudsen KA
    Developmental biology 185.1 (1997 May 1): 14-24.

    Integration of left-right Pitx2 transcription and Wnt signaling drives asymmetric gut morphogenesis via Daam2.
    Kurpios NA
    Developmental cell 26.6 (2013 Sep 30): 629-44.

    Notch1 signaling regulates chondrogenic lineage determination through Sox9 activation.
    Rohwedel J
    Cell death and differentiation 19.3 (2012 Mar): 461-9.

    Comparative analysis of glucagonergic cells, glia, and the circumferential marginal zone in the reptilian retina.
    Fischer AJ
    The Journal of comparative neurology 524.1 (2016 Jan 1): 74-89.

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