6B3
Catalog Fields
Product Name/ID: 6B3
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: CDH2
Ab Isotype: MIgG1, kappa light chain
Gene Symbol:
Entrez Gene ID: 414745
Clonality: Monoclonal
Immunogen: Day 11 chick embryo heart extract
Clone:
Immunogen Sequence: Affinity purified N-cadherin/catenin complex
Myeloma Strain: P3/NSI/1-AG4-1
Epitope Mapped: Yes
Antigen Name: Cadherin, N- (neural)
Epitope Location or Sequence: The antibody binds the extracellular domain of N-cadherin.
Alternate Antigen Name:
Deposit Date: 10/25/2005
Antigen Molecular Weight: Predicted: 82 kDa; Apparent: 135 kDa (full length), 100-kDa (extracellular fragment)
Depositor: Knudsen, K.A.
Antigen Sequence:
Depositor Institution: Lankenau Institute for Medical Research
Antigen Species: Chicken
Depositor Notes: This antibody does not recognize other cadherins including R-cadherin or mouse N-cadherin.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Chicken, Zebrafish
Additional Information: RRID:AB_528118
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B3 was deposited to the DSHB by Knudsen, K.A. (DSHB Hybridoma Product 6B3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
13 References
Initial Publication
N-cadherin promotes the commitment and differentiation of skeletal muscle precursor cells.
Knudsen KA
Developmental biology 185.1 (1997 May 1): 14-24.