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SKU: 1h5
View product citations for antibody 1h5 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: cytokeratin type II (Xenopus)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Klymkowsky, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Molecular, Cellular & Developmental Biology, University of Colorado, Boulder
Positive Tested Species Reactivity: Chicken, Xenopus
Depositors Notes: The antibody induces the disruption of cytokeratin organization when injected into A6 cells. no dramatic effect when injected into embryos. Immunohistochemistry stains cortical cytokeratin system of early Xenoopus embryo. The specificity of the antibody is fro type II cytokeratins of Xenopus early embryo.
Antigen Molecular Weight: 59 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: insoluble residues of Xenopus A6 (kidney epithelial) cells
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: P3
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_528323 
Additional Information: The immunization route is multiple/intraperitoneal using RIBI adjuvant. RRID:AB_528323
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
1h5 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 1h5)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

2 References

  • Initial Publication
  • All References
  • Initial Publication
    All References

    Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos.
    Polson AG
    Development (Cambridge, England) 100.3 (1987 Jul): 543-57.

    Isolated vegetal cortex from Xenopus oocytes selectively retains localized mRNAs.
    Forristall C
    Developmental biology 160.2 (1993 Dec): 554-62.

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