14h7
Catalog Fields
Clone ID/Product Name: 14h7
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Symbol: vim1 & vim4
Ab Isotype: MIgG1
Gene Name:
Clonality: Monoclonal
Immunogen: Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments)
Clone:
Immunogen Sequence: Full length protein
Myeloma Strain: P3
Epitope Mapped: No
Antigen Name: vimentin (Xenopus)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 11/1/1988
Antigen Molecular Weight: Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa
Depositor: Klymkowsky, M.
Antigen Sequence:
Depositor Institution: University of Colorado / MCD Biology
Antigen Species: Xenopus
Depositor Notes: This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.
Host Species: Mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot