Clone ID / Name: 14h7
Anti - vimentin (Xenopus)
Immunofluorescent image of stage 57 X. laevis transgenic tadpole progenitor cells in the tectum labeled with DSHB antibody 14h7 against vimentin (green). PMID: 28795134, Fig. 1E.
Catalog Fields
Identity
Clone ID / Name: 14h7
Alternate Antibody Name: —
Antigen Name: vimentin (Xenopus)
Alternate Antigen Name: —
Gene Symbol: —
Gene Name: vim1 & vim4
Antigen Sequence: —
Antigen Species: Xenopus
Antigen Mol. Weight: Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa
Human Protein Atlas: —
Antibody Properties
Host Species: Mouse
Clonality: Monoclonal
Isotype: MIgG1
Myeloma Strain: P3
Antibody Registry / RRID: AB_528507
Available to For-Profits: Yes
Hybridoma Available: Yes
Cell lines cannot be distributed to commercial/for-profit users.
Cell lines cannot be distributed to commercial/for-profit users.
Immunogen & Epitope
Immunogen: Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments)
Immunogen Sequence: Full length protein
Epitope Mapped: No
Epitope Location / Seq: —
Clone: —
Additional Info: While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively.
Add. Characterization: —
Species Reactivity
Confirmed Reactivity: Xenopus
Predicted Reactivity: —
Depositor
Depositor Name: M. Klymkowsky
Institution: University of Colorado / MCD Biology
Deposit Date: November 1, 1988
Depositor Notes: This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.
Recommended Applications
Function Blocking
IF
IHC
WB
Each lab must optimize concentration per application and species. Full usage recommendations →