14h7
Catalog Fields
Antigen: vimentin (Xenopus)
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Klymkowsky, M.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Molecular, Cellular & Developmental Biology, University of Colorado
Positive Tested Species Reactivity: Xenopus
Depositors Notes: This antibody appears to be Xenopus-specific and doesn't cross react with mouse. It recognizes the 55 and 57 kDa vimentin proteins encoded by the genes vim1 and vim4, respectively. 14h7 disrupts vimentin organization when injected into Xenopus A6 cells. 14H7 undergoes frequent class switching from IgG1 to IgG2a.
Antigen Molecular Weight: Predicted: 52.8 (vim1) and 53.5 (vim4) kDa; Apparent: 55 (vim1) and 57 (vim4) kDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: vim1 & vim4
Immunogen: Cell insoluble residues derived from the Xenopus kidney epithelial cell line A6 (mainly containing intermediate filaments)
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: P3
Epitope Location or Sequence:
Uniprot ID: P24789 P24790
Immunogen Sequence: Full length protein
Entrez Gene ID: 386601 386600
Additional Characterization:
Antibody Registry ID: AB_528507
Additional Information: While this antibody has been reported to be Xenopus-specific, Arciniegas et al. [PMID: 10603448] and Gomes et al. [PMID: 22075471] have reported using it succesfully on chicken embryos and mouse cultured ectoplacental cones, respectively.
Recommended Applications: Function Blocking, Immunofluorescence, Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
14h7 was deposited to the DSHB by Klymkowsky, M. (DSHB Hybridoma Product 14h7)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
10 References
Initial Publication
Polar asymmetry in the organization of the cortical cytokeratin system of Xenopus laevis oocytes and embryos.
Polson AG
Development (Cambridge, England) 100.3 (1987 Jul): 543-57.