5D3
Catalog Fields
Antigen: Cadherin, E-
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Isotype: MIgG2b
Depositors Institution: University of Virginia
Host Species: mouse
Depositors Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Positive Tested Species Reactivity: Drosophila, Xenopus
Gene: Cdh1
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Alternate Gene Names: arc-1/cd324/cdhe/ecad/lcam/uvo
Myeloma Strain: sp-2/0
Clonality: Monoclonal
Uniprot ID: P30944
Epitope Mapped: Yes
Entrez Gene ID: 100337618
Epitope Location or Sequence: Extracellular domain
Antibody Registry ID: AB_528116
Immunogen Sequence: partial protein
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D3 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 5D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
12 References
Initial Publication
Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
Gumbiner B
The Journal of cell biology 108.6 (1989 Jun): 2449-58.