5D3

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$40.00
SKU: 5D3
View product citations for antibody 5D3 on CiteAb

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Available: 201

DSHB Data Sheet

Catalog Fields

Antigen: Cadherin, E-
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Drosophila, Xenopus
Depositors Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Antigen Molecular Weight:
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: Cdh1
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Alternate Gene Names: arc-1/cd324/cdhe/ecad/lcam/uvo
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: sp-2/0
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P30944 
Immunogen Sequence: partial protein
Entrez Gene ID: 100337618 
Additional Characterization:
Antibody Registry ID: AB_528116 
Additional Information: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D3 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 5D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

12 References

  • Initial Publication
  • IF References
  • WB References
  • IP References
  • FFPE References
  • FB References
  • Epitope Map References
  • All References
  • Initial Publication
    IF References

    Radial intercalation of ciliated cells during Xenopus skin development.
    Kintner C
    Development (Cambridge, England) 133.13 (2006 Jul): 2507-15.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Specification of ion transport cells in the Xenopus larval skin.
    Kintner C
    Development (Cambridge, England) 138.4 (2011 Feb): 705-14.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    The distribution of E-cadherin during Xenopus laevis development.
    Thiery JP
    Development (Cambridge, England) 111.1 (1991 Jan): 159-69.

    Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.
    Asashima M
    Advances in space research : the official journal of the Committee on Space Research (COSPAR) 35.9 (2005): 1654-61.

    Chase-and-run between adjacent cell populations promotes directional collective migration.
    Mayor R
    Nature cell biology 15.7 (2013 Jul): 763-72.

    Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
    Mayor R
    Developmental cell 34.4 (2015 Aug 24): 421-34.

    Visualization of adult stem cells within their niches using the Drosophila germline as a model system.
    Shcherbata HR
    Methods in molecular biology (Clifton, N.J.) 1035. (2013): 25-33.

    WB References
    IP References
    FFPE References

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular and developmental biology. Animal 49.3 (2013 Mar): 220-9.

    FB References
    Epitope Map References
    All References

    Radial intercalation of ciliated cells during Xenopus skin development.
    Kintner C
    Development (Cambridge, England) 133.13 (2006 Jul): 2507-15.

    Specializations of intercellular junctions are associated with the presence and absence of hair cell regeneration in ears from six vertebrate classes.
    Corwin JT
    The Journal of comparative neurology 521.6 (2013 Apr 15): 1430-48.

    Specification of ion transport cells in the Xenopus larval skin.
    Kintner C
    Development (Cambridge, England) 138.4 (2011 Feb): 705-14.

    Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
    Gumbiner B
    The Journal of cell biology 108.6 (1989 Jun): 2449-58.

    The distribution of E-cadherin during Xenopus laevis development.
    Thiery JP
    Development (Cambridge, England) 111.1 (1991 Jan): 159-69.

    Temporal regulation of global gene expression and cellular morphology in Xenopus kidney cells in response to clinorotation.
    Asashima M
    Advances in space research : the official journal of the Committee on Space Research (COSPAR) 35.9 (2005): 1654-61.

    Chase-and-run between adjacent cell populations promotes directional collective migration.
    Mayor R
    Nature cell biology 15.7 (2013 Jul): 763-72.

    Cadherin Switch during EMT in Neural Crest Cells Leads to Contact Inhibition of Locomotion via Repolarization of Forces.
    Mayor R
    Developmental cell 34.4 (2015 Aug 24): 421-34.

    Visualization of adult stem cells within their niches using the Drosophila germline as a model system.
    Shcherbata HR
    Methods in molecular biology (Clifton, N.J.) 1035. (2013): 25-33.

    Foregut mesenchyme contributes cells to pancreatic acini during embryonic development in a chick-quail chimera model.
    Johnson PR
    Pediatric surgery international 21.3 (2005 Mar): 138-42.

    Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
    Gumbiner BM
    Development (Cambridge, England) 120.4 (1994 Apr): 901-9.

    The cell sorting process of Xenopus gastrula cells involves the acto-myosin system and TGF-β signaling.
    Ihara S
    In vitro cellular and developmental biology. Animal 49.3 (2013 Mar): 220-9.

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