5D3
Catalog Fields
Antigen: Cadherin, E-
Hybridoma Cells Available: Yes
Antigen Species: Xenopus
Depositor: Gumbiner, B.M.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: University of Virginia
Positive Tested Species Reactivity: Drosophila, Xenopus
Depositors Notes: Blocks epithelial cell-cell adhesion and cell junction formation. Stains ectoderm, all epithelia and regions of cell-cell contact. E-cadherin-specific by immunoblotting and immunoprecipitation.
Predicted Species Reactivity:
Gene: Cdh1
Immunogen: Purified E-cadherin extracellular domain (100 kDa trypsin fragment ectodomain) from Xenopus A6 kidney cell line.
Alternate Gene Names: arc-1/cd324/cdhe/ecad/lcam/uvo
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: sp-2/0
Epitope Location or Sequence: Extracellular domain
Uniprot ID: P30944
Immunogen Sequence: partial protein
Entrez Gene ID: 100337618
Antibody Registry ID: AB_528116
Additional Information: 5D3 binds both the - and + (Ca++) conformations of E-cadherin
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
5D3 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 5D3)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.
13 References
Initial Publication
Expression of cell adhesion molecule E-cadherin in Xenopus embryos begins at gastrulation and predominates in the ectoderm.
Gumbiner B
The Journal of cell biology 108.6 (1989 Jun): 2449-58.