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SKU: 20B4
View product citations for antibody 20B4 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: neural crest cells
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Denburg, J.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: Department of Biological Sciences, University of Iowa,
Positive Tested Species Reactivity: Chicken
Depositors Notes: Immunohistochemistry was performed frozen fixed (4% paraformaldehyde) sections; migrating neural crest, DRG, outer ectodermal layer (except neural tube).
Antigen Molecular Weight:
Human Protein Atlas:  
Predicted Species Reactivity:  
Immunogen: chick embryonic spinal cord; crude membrane preparation
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: NS1
Epitope Location or Sequence:
Uniprot ID:  
Immunogen Sequence:
Entrez Gene ID:  
Additional Characterization:  
Antibody Registry ID: AB_531868 
Additional Information: Immunization was on 12/8/84, 12/31/84, 1/24/85, and 2/15/85 using 600 micrograms of protien injection 3 days after each boost. RRID:AB_531868
Recommended Applications: Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
20B4 was deposited to the DSHB by Denburg, J. (DSHB Hybridoma Product 20B4)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.

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