2F5-1
Catalog Fields
Antigen: FMR1 (residues #1-204)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Tartakoff, A.M. / Fallon, J.R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Case Western Reserve University
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Recommended starting concentration of 5-10 ug/ml for immunocytochemistry and immunohistochemistry. Staining of aldehyde fixed tissue requires antigen retrieval (Christie et al., 2009).
Predicted Species Reactivity:
Gene: FMR1
Immunogen: N-terminal fragment of human FMRP-6X-His-tagged fusion protein.
Alternate Gene Names: FMRP, FRAXA, POF, POF1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: a.a. 1–204 (N-terminus)
Uniprot ID: Q06787
Immunogen Sequence: (residues #1-204)
Entrez Gene ID: 2332
Antibody Registry ID: AB_10805421
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F5-1 was deposited to the DSHB by Tartakoff, A.M. / Fallon, J.R. (DSHB Hybridoma Product 2F5-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
Although the optimal Ig concentration for an application varies for each product and must be optimized for each laboratory, a good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) is 2-5 ug/ml. For western blots, the recommended concentration range is 0.2-0.5 ug/ml.
All cell products contain the antimicrobial ProClin. Click here for additional information.
20 References
Initial Publication
Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
Fallon JR
The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.