2F5-1
Catalog Fields
Antigen: FMR1 (residues #1-204)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Tartakoff, A.M. / Fallon, J.R.
Isotype: MIgG2b
Antigen Sequence:
Host Species: mouse
Depositors Institution: Case Western Reserve University
Positive Tested Species Reactivity: Human, Mouse, Rat
Depositors Notes: Recommended starting concentration of 5-10 ug/ml for immunocytochemistry and immunohistochemistry. Staining of aldehyde fixed tissue requires antigen retrieval (Christie et al., 2009).
Antigen Molecular Weight: Predicted: 71KDa
Human Protein Atlas:
Predicted Species Reactivity:
Gene: FMR1
Immunogen: N-terminal fragment of human FMRP-6X-His-tagged fusion protein.
Alternate Gene Names: FMRP, FRAXA, POF, POF1
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: Yes
Myeloma Strain: NS1
Epitope Location or Sequence: a.a. 1–204 (N-terminus)
Uniprot ID: Q06787
Immunogen Sequence: (residues #1-204)
Entrez Gene ID: 2332
Additional Characterization:
Antibody Registry ID: AB_10805421
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F5-1 was deposited to the DSHB by Tartakoff, A.M. / Fallon, J.R. (DSHB Hybridoma Product 2F5-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
19 References
Initial Publication
Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
Fallon JR
The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.