2F5-1

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$40.00
SKU: 2F5-1
View product citations for antibody 2F5-1 on CiteAb

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DSHB Data Sheet
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Catalog Fields

Antigen: FMR1 (residues #1-204)
Hybridoma Cells Available: Yes
Antigen Species: Human
Depositor: Tartakoff, A.M. / Fallon, J.R.
Isotype: MIgG2b
Depositors Institution: Case Western Reserve University
Host Species: mouse
Depositors Notes: Recommended starting concentration of 5-10 ug/ml for immunocytochemistry and immunohistochemistry. Staining of aldehyde fixed tissue requires antigen retrieval (Christie et al., 2009).
Positive Tested Species Reactivity: Human, Mouse, Rat
Gene: FMR1
Antigen Molecular Weight: Predicted: 71KDa
Alternate Gene Names: FMRP, FRAXA, POF, POF1
Immunogen: N-terminal fragment of human FMRP-6X-His-tagged fusion protein.
Clonality: Monoclonal
Myeloma Strain: NS1
Epitope Mapped: Yes
Uniprot ID: Q06787 
Epitope Location or Sequence: a.a. 1–204 (N-terminus)
Entrez Gene ID: 2332 
Immunogen Sequence: (residues #1-204)
Antibody Registry ID: AB_10805421 
Recommended Applications: Immunofluorescence, Immunohistochemistry
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These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F5-1 was deposited to the DSHB by Tartakoff, A.M. / Fallon, J.R. (DSHB Hybridoma Product 2F5-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

19 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • Epitope Map References
  • All References
    • Initial Publication

    Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.

    IF References

    Netrin-1 induces local translation of down syndrome cell adhesion molecule in axonal growth cones.
    Welshhans K
    Developmental neurobiology 76.7 (2016 Jul): 799-816.

    Deletion of Fmr1 from Forebrain Excitatory Neurons Triggers Abnormal Cellular, EEG, and Behavioral Phenotypes in the Auditory Cortex of a Mouse Model of Fragile X Syndrome.
    Razak KA
    Cerebral cortex (New York, N.Y. : 1991) . (2019 Jul 29): .

    WB References

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Astroglial FMRP-dependent translational down-regulation of mGluR5 underlies glutamate transporter GLT1 dysregulation in the fragile X mouse.
    Yang Y
    Human molecular genetics 22.10 (2013 May 15): 2041-54.

    Increased GABA(B) receptor-mediated signaling reduces the susceptibility of fragile X knockout mice to audiogenic seizures.
    Hampson DR
    Molecular pharmacology 76.1 (2009 Jul): 18-24.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

    IHC References

    Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    Astroglial FMRP-dependent translational down-regulation of mGluR5 underlies glutamate transporter GLT1 dysregulation in the fragile X mouse.
    Yang Y
    Human molecular genetics 22.10 (2013 May 15): 2041-54.

    Delayed myelination in a mouse model of fragile X syndrome.
    Hampson DR
    Human molecular genetics 22.19 (2013 Oct 1): 3920-30.

    Reduced phenotypic severity following adeno-associated virus-mediated Fmr1 gene delivery in fragile X mice.
    Hampson DR
    Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 39.13 (2014 Dec): 3100-11.

    Venous endothelin guides sympathetic innervation of the developing mouse heart.
    Makita T
    Nature communications 5. (2014 May 29): 3918.

    Tissue penetration and activity of camptothecins in solid tumor xenografts.
    Minchinton AI
    Molecular cancer therapeutics 13.11 (2014 Nov): 2727-37.

    Geissoschizine methyl ether, an alkaloid from the Uncaria hook, improves remyelination after cuprizone-induced demyelination in medial prefrontal cortex of adult mice.
    Wanaka A
    Neurochemical research 39.1 (2014 Jan): 59-67.

    Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation.
    Miyata S
    Cell biochemistry and function 32.1 (2014 Jan): 51-61.

    Antidepressant-induced vascular dynamics in the hippocampus of adult mouse brain.
    Miyata S
    Cell and tissue research 358.1 (2014 Oct): 43-55.

    VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice.
    Miyata S
    The Journal of endocrinology 222.1 (2014 Jul): 161-79.

    Hox11 genes are required for regional patterning and integration of muscle, tendon and bone.
    Wellik DM
    Development (Cambridge, England) 140.22 (2013 Nov): 4574-82.

    A functional requirement for astroglia in promoting blood vessel development in the early postnatal brain.
    Huang Z
    PloS one 7.10 (2012): e48001.

    An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo.
    Ogita H
    PloS one 8.6 (2013): e68134.

    Epitope Map References

    Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.

    All References

    Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    Astroglial FMRP-dependent translational down-regulation of mGluR5 underlies glutamate transporter GLT1 dysregulation in the fragile X mouse.
    Yang Y
    Human molecular genetics 22.10 (2013 May 15): 2041-54.

    Delayed myelination in a mouse model of fragile X syndrome.
    Hampson DR
    Human molecular genetics 22.19 (2013 Oct 1): 3920-30.

    Reduced phenotypic severity following adeno-associated virus-mediated Fmr1 gene delivery in fragile X mice.
    Hampson DR
    Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 39.13 (2014 Dec): 3100-11.

    Venous endothelin guides sympathetic innervation of the developing mouse heart.
    Makita T
    Nature communications 5. (2014 May 29): 3918.

    Tissue penetration and activity of camptothecins in solid tumor xenografts.
    Minchinton AI
    Molecular cancer therapeutics 13.11 (2014 Nov): 2727-37.

    Geissoschizine methyl ether, an alkaloid from the Uncaria hook, improves remyelination after cuprizone-induced demyelination in medial prefrontal cortex of adult mice.
    Wanaka A
    Neurochemical research 39.1 (2014 Jan): 59-67.

    Changes in pericytic expression of NG2 and PDGFRB and vascular permeability in the sensory circumventricular organs of adult mouse by osmotic stimulation.
    Miyata S
    Cell biochemistry and function 32.1 (2014 Jan): 51-61.

    Antidepressant-induced vascular dynamics in the hippocampus of adult mouse brain.
    Miyata S
    Cell and tissue research 358.1 (2014 Oct): 43-55.

    VEGF-dependent and PDGF-dependent dynamic neurovascular reconstruction in the neurohypophysis of adult mice.
    Miyata S
    The Journal of endocrinology 222.1 (2014 Jul): 161-79.

    Hox11 genes are required for regional patterning and integration of muscle, tendon and bone.
    Wellik DM
    Development (Cambridge, England) 140.22 (2013 Nov): 4574-82.

    A functional requirement for astroglia in promoting blood vessel development in the early postnatal brain.
    Huang Z
    PloS one 7.10 (2012): e48001.

    An Adaptor Molecule Afadin Regulates Lymphangiogenesis by Modulating RhoA Activity in the Developing Mouse Embryo.
    Ogita H
    PloS one 8.6 (2013): e68134.

    Netrin-1 induces local translation of down syndrome cell adhesion molecule in axonal growth cones.
    Welshhans K
    Developmental neurobiology 76.7 (2016 Jul): 799-816.

    Deletion of Fmr1 from Forebrain Excitatory Neurons Triggers Abnormal Cellular, EEG, and Behavioral Phenotypes in the Auditory Cortex of a Mouse Model of Fragile X Syndrome.
    Razak KA
    Cerebral cortex (New York, N.Y. : 1991) . (2019 Jul 29): .

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Increased GABA(B) receptor-mediated signaling reduces the susceptibility of fragile X knockout mice to audiogenic seizures.
    Hampson DR
    Molecular pharmacology 76.1 (2009 Jul): 18-24.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

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