2F5-1
Catalog Fields
Product Name/ID: 2F5-1
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: FMR1
Ab Isotype: MIgG2b
Gene Symbol:
Antibody Registry ID: AB_10805421
Uniprot ID: Q06787
Entrez Gene ID: 2332
Clonality: Monoclonal
Immunogen: N-terminal fragment of human FMRP-6X-His-tagged fusion protein.
Clone:
Immunogen Sequence: (residues #1-204)
Myeloma Strain: NS1
Epitope Mapped: Yes
Antigen Name: FMR1 (residues #1-204)
Epitope Location or Sequence: a.a. 1–204 (N-terminus)
Alternate Antigen Name:
Deposit Date: 9/10/2010
Antigen Molecular Weight: Predicted: 71KDa
Depositor: Tartakoff, A.M. / Fallon, J.R.
Antigen Sequence:
Depositor Institution: Case Western Reserve University
Antigen Species: Human
Depositor Notes: Recommended starting concentration of 5-10 ug/ml for immunocytochemistry and immunohistochemistry. Staining of aldehyde fixed tissue requires antigen retrieval (Christie et al., 2009).
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Human, Mouse, Rat
Additional Information:
Recommended Applications: Immunofluorescence, Immunohistochemistry
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
2F5-1 was deposited to the DSHB by Tartakoff, A.M. / Fallon, J.R. (DSHB Hybridoma Product 2F5-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
20 References
Initial Publication
Visual experience regulates transient expression and dendritic localization of fragile X mental retardation protein.
Fallon JR
The Journal of neuroscience : the official journal of the Society for Neuroscience 24.47 (2004 Nov 24): 10579-83.