7G1-1
Catalog Fields
Antigen: FMR1
Depositor: Warren, S.T.
Antigen Species: Mouse
Depositors Institution: Emory University
Isotype: MIgG2b
Gene: Fmr1
Host Species: mouse
Alternate Gene Names: FMRP, Fmr-1
Positive Tested Species Reactivity: Mouse, Rat
Clonality: Monoclonal
Antigen Molecular Weight: ~70-80kDa
Epitope Mapped: Yes
Immunogen: Fmrp-6X-his fusion protein.
Epitope Location or Sequence: a.a. 354-368 [seq: KHLDTKENTHFSQPN}
Myeloma Strain: Sp2/0
Uniprot ID: P35922
Entrez Gene ID: 14265
Antibody Registry ID: AB_528251
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7G1-1 was deposited to the DSHB by Warren, S.T. (DSHB Hybridoma Product 7G1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.
22 References
Initial Publication
Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
Warren ST
Cell 107.4 (2001 Nov 16): 477-87.