7G1-1

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SKU: 7G1-1
View product citations for antibody 7G1-1 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: FMR1
Depositor: Warren, S.T.
Antigen Species: Mouse
Depositors Institution: Emory University
Isotype: MIgG2b
Gene: Fmr1
Host Species: mouse
Alternate Gene Names: FMRP, Fmr-1
Positive Tested Species Reactivity: Mouse, Rat
Clonality: Monoclonal
Antigen Molecular Weight: ~70-80kDa
Epitope Mapped: Yes
Immunogen: Fmrp-6X-his fusion protein.
Epitope Location or Sequence: a.a. 354-368 [seq: KHLDTKENTHFSQPN}
Myeloma Strain: Sp2/0
Uniprot ID: P35922 
Entrez Gene ID: 14265 
Antibody Registry ID: AB_528251 
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7G1-1 was deposited to the DSHB by Warren, S.T. (DSHB Hybridoma Product 7G1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

22 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • Epitope Map References
  • Gel Supershift References
  • All References
  • Initial Publication
    IF References

    Myosin Va is required for the transport of fragile X mental retardation protein (FMRP) granules.
    McCaffrey MW
    Biology of the cell 106.2 (2014 Feb): 57-71.

    Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
    Boccaccio GL
    The Journal of cell biology 195.7 (2011 Dec 26): 1141-57.

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    WB References
    IHC References

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    IP References

    Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
    Warren ST
    Cell 107.4 (2001 Nov 16): 477-87.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density.
    Dolphin AC
    Nature communications 5. (2014 Apr 7): 3628.

    Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures.
    Benke TA
    Neurobiology of disease 59. (2013 Nov): 1-17.

    Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3.1b.
    Kaczmarek LK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10263-71.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism.
    Darnell RB
    Cell 146.2 (2011 Jul 22): 247-61.

    Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus.
    Wong RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.2 (2011 Jan 12): 725-34.

    An alternative mode of microRNA target recognition.
    Darnell RB
    Nature structural & molecular biology 19.3 (2012 Feb 12): 321-7.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

    FMRP mediates mGluR5-dependent translation of amyloid precursor protein.
    Malter JS
    PLoS biology 5.3 (2007 Mar): e52.

    Reply to Bagni: On BC1 RNA and the fragile X mental retardation protein.
    Tiedge H
    Proceedings of the National Academy of Sciences of the United States of America 105.22 (2008 Jun 3): E29.

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain.
    Smalheiser NR
    Journal of neurochemistry 106.2 (2008 Jul): 650-61.

    Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
    Dziembowska M
    Molecular neurobiology 56.4 (2019 Apr): 2741-2759.

    Epitope Map References
    Gel Supershift References

    Post-transcriptional regulation of the let-7 microRNA during neural cell specification.
    Nitsch R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 415-26.

    All References

    The FXG: a presynaptic fragile X granule expressed in a subset of developing brain circuits.
    Fallon JR
    The Journal of neuroscience : the official journal of the Society for Neuroscience 29.5 (2009 Feb 4): 1514-24.

    Myosin Va is required for the transport of fragile X mental retardation protein (FMRP) granules.
    McCaffrey MW
    Biology of the cell 106.2 (2014 Feb): 57-71.

    Smaug1 mRNA-silencing foci respond to NMDA and modulate synapse formation.
    Boccaccio GL
    The Journal of cell biology 195.7 (2011 Dec 26): 1141-57.

    Fragile X mental retardation protein interacts with the RNA-binding protein Caprin1 in neuronal RiboNucleoProtein complexes [corrected].
    Khandjian EW
    PloS one 7.6 (2012): e39338.

    FXR1P but not FMRP regulates the levels of mammalian brain-specific microRNA-9 and microRNA-124.
    Gao FB
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.39 (2011 Sep 28): 13705-9.

    FMRP regulates the transition from radial glial cells to intermediate progenitor cells during neocortical development.
    Xie Z
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.4 (2011 Jan 26): 1427-39.

    Genetic deletion of regulator of G-protein signaling 4 (RGS4) rescues a subset of fragile X related phenotypes in the FMR1 knockout mouse.
    Hampson DR
    Molecular and cellular neurosciences 46.3 (2011 Mar): 563-72.

    The RNA binding and transport proteins staufen and fragile X mental retardation protein are expressed by rat primary afferent neurons and localize to peripheral and central axons.
    Hargreaves KM
    Neuroscience 141.4 (2006 Sep 15): 2107-16.

    A mouse model of the human Fragile X syndrome I304N mutation.
    Darnell RB
    PLoS genetics 5.12 (2009 Dec): e1000758.

    Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
    Warren ST
    Cell 107.4 (2001 Nov 16): 477-87.

    Fragile X mental retardation protein controls synaptic vesicle exocytosis by modulating N-type calcium channel density.
    Dolphin AC
    Nature communications 5. (2014 Apr 7): 3628.

    Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures.
    Benke TA
    Neurobiology of disease 59. (2013 Nov): 1-17.

    Fragile X mental retardation protein is required for rapid experience-dependent regulation of the potassium channel Kv3.1b.
    Kaczmarek LK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.31 (2010 Aug 4): 10263-71.

    FMRP stalls ribosomal translocation on mRNAs linked to synaptic function and autism.
    Darnell RB
    Cell 146.2 (2011 Jul 22): 247-61.

    Dual regulation of fragile X mental retardation protein by group I metabotropic glutamate receptors controls translation-dependent epileptogenesis in the hippocampus.
    Wong RK
    The Journal of neuroscience : the official journal of the Society for Neuroscience 31.2 (2011 Jan 12): 725-34.

    An alternative mode of microRNA target recognition.
    Darnell RB
    Nature structural & molecular biology 19.3 (2012 Feb 12): 321-7.

    Excess phosphoinositide 3-kinase subunit synthesis and activity as a novel therapeutic target in fragile X syndrome.
    Bassell GJ
    The Journal of neuroscience : the official journal of the Society for Neuroscience 30.32 (2010 Aug 11): 10624-38.

    FMRP mediates mGluR5-dependent translation of amyloid precursor protein.
    Malter JS
    PLoS biology 5.3 (2007 Mar): e52.

    Reply to Bagni: On BC1 RNA and the fragile X mental retardation protein.
    Tiedge H
    Proceedings of the National Academy of Sciences of the United States of America 105.22 (2008 Jun 3): E29.

    Expression of microRNAs and their precursors in synaptic fractions of adult mouse forebrain.
    Smalheiser NR
    Journal of neurochemistry 106.2 (2008 Jul): 650-61.

    Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage.
    Dziembowska M
    Molecular neurobiology 56.4 (2019 Apr): 2741-2759.

    Post-transcriptional regulation of the let-7 microRNA during neural cell specification.
    Nitsch R
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology 21.2 (2007 Feb): 415-26.

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