7G1-1
Catalog Fields
Antigen: FMR1
Available to For-Profits: Yes
Antigen Species: Mouse
Hybridoma Cells Available (Non-Profit): No
Isotype: MIgG2b
Depositor: Warren, S.T.
Host Species: mouse
Antigen Sequence:
Positive Tested Species Reactivity: Mouse, Rat
Depositors Institution: Emory University
Antigen Molecular Weight: ~70-80kDa
Depositors Notes:
Immunogen: Fmrp-6X-his fusion protein.
Gene: Fmr1
Alternate Antibody Name:
Alternate Gene Names: FMRP, Fmr-1
Alternate Antigen Name:
Clonality: Monoclonal
Myeloma Strain: Sp2/0
Epitope Mapped: Yes
Uniprot ID: P35922
Epitope Location or Sequence: a.a. 354-368 [seq: KHLDTKENTHFSQPN}
Entrez Gene ID: 14265
Immunogen Sequence:
Antibody Registry ID: AB_528251
Recommended Applications: Immunofluorescence, Immunoprecipitation, Western Blot
Additional Information:
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
7G1-1 was deposited to the DSHB by Warren, S.T. (DSHB Hybridoma Product 7G1-1)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
23 References
Initial Publication
Microarray identification of FMRP-associated brain mRNAs and altered mRNA translational profiles in fragile X syndrome.
Warren ST
Cell 107.4 (2001 Nov 16): 477-87.