31 or 31-2

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$40.00
SKU: 31 or 31-2
View product citations for antibody 31 or 31-2 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: laminin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University
Positive Tested Species Reactivity: Chicken, Guineafowl, Quail
Depositors Notes: Doesn't precipitate mouse laminin.
Antigen Molecular Weight: Apparent: 200 and 400 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMB1
Immunogen: crude preparation of laminin from chick muscle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID: F1NJ23 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396478 
Additional Characterization:
Antibody Registry ID: AB_528341 
Additional Information: The gene, Uniprot ID and gene ID associated with this mAb are not exclusive since 31-2 has been reported to recognize alpha and beta laminins.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
31 or 31-2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 31 or 31-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    The Journal of cell biology
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    IF References

    The Journal of cell biology
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    Development (Cambridge, England)
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    Anatomy and embryology
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Journal of cell science
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    The Anatomical record
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    Microscopy research and technique
    Yablonka-Reuveni Z
    Microscopy research and technique 30.5 (1995 Apr 1): 366-80.

    Anatomy and embryology
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Developmental biology
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Journal of cell science
    Gammill LS
    Journal of cell science 126.Pt 6 (2013 Mar 15): 1464-76.

    WB References

    Developmental biology
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    IHC References

    Cell and tissue research
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Developmental biology
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Avian pathology : journal of the W.V.P.A
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    Developmental biology
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Tissue engineering
    Lamont SE
    Tissue engineering 9.6 (2003 Dec): 1271-9.

    IP References

    The Journal of cell biology
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    All References

    Cell and tissue research
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Developmental biology
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Avian pathology : journal of the W.V.P.A
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    Developmental biology
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Tissue engineering
    Lamont SE
    Tissue engineering 9.6 (2003 Dec): 1271-9.

    The Journal of cell biology
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    Development (Cambridge, England)
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    Anatomy and embryology
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Journal of cell science
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    The Anatomical record
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Developmental dynamics : an official publication of the American Association of Anatomists
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    Microscopy research and technique
    Yablonka-Reuveni Z
    Microscopy research and technique 30.5 (1995 Apr 1): 366-80.

    Anatomy and embryology
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Journal of cell science
    Gammill LS
    Journal of cell science 126.Pt 6 (2013 Mar 15): 1464-76.

    Cellular & molecular biology research
    Christ B
    Cellular & molecular biology research 41.4 (1995): 241-9.

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