31 or 31-2

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$40.00
SKU: 31 or 31-2
View product citations for antibody 31 or 31-2 on CiteAb

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Available: 36

DSHB Data Sheet

Catalog Fields

Antigen: laminin
Hybridoma Cells Available: Yes
Antigen Species: chicken
Depositor: Fambrough, D.M.
Isotype: MIgG1, kappa light chain
Antigen Sequence:
Host Species: mouse
Depositors Institution: The Johns Hopkins University
Positive Tested Species Reactivity: Chicken, Guineafowl, Quail
Depositors Notes: Doesn't precipitate mouse laminin.
Antigen Molecular Weight: Apparent: 200 and 400 kDa
Human Protein Atlas:
Predicted Species Reactivity:  
Gene: LAMB1
Immunogen: crude preparation of laminin from chick muscle
Alternate Gene Names:
Alternate Antibody Name:
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped:
Myeloma Strain: SP2/0 Ag-14
Epitope Location or Sequence:
Uniprot ID: F1NJ23 
Immunogen Sequence: Full length protein
Entrez Gene ID: 396478 
Additional Characterization:
Antibody Registry ID: AB_528341 
Additional Information: The gene, Uniprot ID and gene ID associated with this mAb are not exclusive since 31-2 has been reported to recognize alpha and beta laminins.
Recommended Applications: Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
31 or 31-2 was deposited to the DSHB by Fambrough, D.M. (DSHB Hybridoma Product 31 or 31-2)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. To ensure retention of antibody activity, we recommend aliquotting the product into two parts: 1) a volume of antibody stored at 4°C to be used within two weeks. 2) the remaining product diluted with an equal volume of molecular grade glycerol and stored at -20°C.
Usage Recommendations
While optimal Ig concentration for an application will vary, a good starting concentration for immunohistochemistry (IHC), immunofluorescence(IF) and staining is 2-5 µg/ml. For Western blots, the concentration is decreased by one order of magnitude (that is, 0.2-0.5 µg/ml).
All cell products contain the antimicrobial ProClin. Click here for additional information.

15 References

  • Initial Publication
  • IF References
  • WB References
  • IHC References
  • IP References
  • All References
  • Initial Publication

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    IF References

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    The accumulation of basement membrane components during the onset of chondrogenesis and myogenesis in the chick wing bud.
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Immunolocalization of basal lamina components during development of chick otic and optic primordia.
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Basal lamina development in chicken muscle spindles.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    Development and postnatal regulation of adult myoblasts.
    Yablonka-Reuveni Z
    Microscopy research and technique 30.5 (1995 Apr 1): 366-80.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Tetraspanin18 is a FoxD3-responsive antagonist of cranial neural crest epithelial-to-mesenchymal transition that maintains cadherin-6B protein.
    Gammill LS
    Journal of cell science 126.Pt 6 (2013 Mar 15): 1464-76.

    WB References
    IHC References
    IP References

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    All References

    Extracellular matrix of different composition supports the various splenic compartments of guinea fowl ( Numida meleagris).
    Oláh I
    Cell and tissue research 312.3 (2003 Jun): 333-43.

    Endothelial cells promote migration and proliferation of enteric neural crest cells via beta1 integrin signaling.
    Goldstein AM
    Developmental biology 330.2 (2009 Jun 15): 263-72.

    Origin of the chicken splenic reticular cells influences the effect of the infectious bursal disease virus on the extracellular matrix.
    Oláh I
    Avian pathology : journal of the W.V.P.A 40.2 (2011 Apr): 199-206.

    Enteric neural crest-derived cells promote their migration by modifying their microenvironment through tenascin-C production.
    Goldstein AM
    Developmental biology 382.2 (2013 Oct 15): 446-56.

    Biomaterial mesh seeded with vascular remnants from a quail embryo has a significant and fast vascular templating effect on host implant tissue.
    Lamont SE
    Tissue engineering 9.6 (2003 Dec): 1271-9.

    Extracellular matrix organization in developing muscle: correlation with acetylcholine receptor aggregates.
    Fambrough DM
    The Journal of cell biology 99.4 Pt 1 (1984 Oct): 1486-501.

    The accumulation of basement membrane components during the onset of chondrogenesis and myogenesis in the chick wing bud.
    Jensen KL
    Development (Cambridge, England) 104.1 (1988 Sep): 41-9.

    The distribution of mesenchyme proteoglycan (PG-M) during wing bud outgrowth.
    Solursh M
    Anatomy and embryology 181.3 (1990): 227-33.

    Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo.
    Mayne R
    Journal of cell science 102 ( Pt 3). (1992 Jul): 643-52.

    Immunolocalization of basal lamina components during development of chick otic and optic primordia.
    Randolph GJ
    The Anatomical record 235.3 (1993 Mar): 443-52.

    Basal lamina development in chicken muscle spindles.
    Mayne R
    Developmental dynamics : an official publication of the American Association of Anatomists 202.3 (1995 Mar): 284-93.

    Development and postnatal regulation of adult myoblasts.
    Yablonka-Reuveni Z
    Microscopy research and technique 30.5 (1995 Apr 1): 366-80.

    Transitions in cell organization and in expression of contractile and extracellular matrix proteins during development of chicken aortic smooth muscle: evidence for a complex spatial and temporal differentiation program.
    Benson JM
    Anatomy and embryology 197.6 (1998 Jun): 421-37.

    Tetraspanin18 is a FoxD3-responsive antagonist of cranial neural crest epithelial-to-mesenchymal transition that maintains cadherin-6B protein.
    Gammill LS
    Journal of cell science 126.Pt 6 (2013 Mar 15): 1464-76.

    Development of chicken aortic smooth muscle: expression of cytoskeletal and basement membrane proteins defines two distinct cell phenotypes emerging from a common lineage.
    Christ B
    Cellular and molecular biology research 41.4 (1995): 241-9.

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