3F8
Catalog Fields
Clone ID/Product Name: 3F8
Available to For-Profits: Yes
Alternate Antibody Name: protein tyrosine phosphatase - z/b
Gene Symbol: PTPRZ1
Ab Isotype: MIgG1
Gene Name:
Entrez Gene ID: 5803
Clonality: Monoclonal
Immunogen: PBS-soluble chondroitin sulfate proteoglycans
Clone:
Immunogen Sequence: Full length core protein sequence
Myeloma Strain: PA1
Epitope Mapped: No
Antigen Name: phosphacan/protein tyrosine phosphatase - z/b
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 1/27/1998
Antigen Molecular Weight: 255.3 kDa
Depositor: Margolis, R.U. / Margolis, R.K.
Antigen Sequence:
Depositor Institution: New York University Medical Center / State University of New York
Antigen Species: rat
Depositor Notes: 3F8 recognizes the perineuronal nets biomarker for phosphacan/RPTP-zeta.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Rat
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3F8 was deposited to the DSHB by Margolis, R.U. / Margolis, R.K. (DSHB Hybridoma Product 3F8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
5 References
Initial Publication
Isolation and characterization of developmentally regulated chondroitin sulfate and chondroitin/keratan sulfate proteoglycans of brain identified with monoclonal antibodies.
Margolis RU
The Journal of biological chemistry 266.22 (1991 Aug 5): 14785-801.