3F8

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$45.00 to $425.00
SKU: 3F8
View product citations for antibody 3F8 on CiteAb

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DSHB Data Sheet

Catalog Fields

Antigen: phosphacan/protein tyrosine phosphatase - z/b
Hybridoma Cells Available: Yes
Antigen Species: rat
Depositor: Margolis, R.U. / Margolis, R.K.
Isotype: MIgG1
Antigen Sequence:
Host Species: mouse
Depositors Institution: New York University Medical Center / State University of New York
Positive Tested Species Reactivity: Rat
Depositors Notes: 3F8 recognizes the perineuronal nets biomarker for phosphacan/RPTP-zeta.
Antigen Molecular Weight: 255.3 kDa
Human Protein Atlas:  
Predicted Species Reactivity:  
Gene: PTPRZ1
Immunogen: PBS-soluble chondroitin sulfate proteoglycans
Alternate Gene Names: Ptprz, Ptpz
Alternate Antibody Name: protein tyrosine phosphatase - z/b
Clonality: Monoclonal
Alternate Antigen Name:
Epitope Mapped: No
Myeloma Strain: PA1
Epitope Location or Sequence:
Uniprot ID: Q62656 
Immunogen Sequence: Full length core protein sequence
Entrez Gene ID: 5803 
Additional Characterization:  
Antibody Registry ID: AB_531799 
Additional Information:
Recommended Applications: Immunohistochemistry, Western Blot
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
3F8 was deposited to the DSHB by Margolis, R.U. / Margolis, R.K. (DSHB Hybridoma Product 3F8)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
All cell products contain the antimicrobial ProClin. Click here for additional information.

5 References

  • Initial Publication
  • WB References
  • IHC References
  • All References
  • Initial Publication
    WB References
    IHC References
    All References

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